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A Novel Matrix Protein p10 from the Nacre of Pearl Oyster (Pinctada fucata) and Its Effects on Both CaCO3 Crystal Formation and Mineralogenic Cells

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Abstract

A novel matrix protein, designated as p10 because of its apparent molecular mass of 10 kDa, was isolated from the nacreous layer of pearl oyster (Pinctada fucata) by reverse-phase high-performance liquid chromatography. In vitro crystallization experiments showed that p10 could accelerate the nucleation of calcium carbonate crystals and induce aragonite formation, suggesting that it might play a key role in nacre biomineralization. As nacre is known to contain osteogenic factors, two mineralogenic cell lines, MRC-5 fibroblasts and MC3T3-E1 preosteoblasts, were used to investigate the biological activity of p10. The results showed that p10 could increase alkaline phosphatase activity, an early marker of osteoblast differentiation, while the viability of MRC-5 and MC3T3-E1 remained unchanged after treatment of p10. Taken together, the findings led to identification of a novel matrix protein from the nacre of P. fucata that plays a role in both the mineral phase and in the differentiation of the cells involved in biomineralization.

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Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (2001AA621140), and the National Natural Science Foundation of China (30170723).

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Authors and Affiliations

  1. Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, People's Republic of China

    Cen Zhang, Shuo Li, Zhuojun Ma, Liping Xie & Rongqing Zhang

  2. Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing, 100084, People's Republic of China

    Liping Xie & Rongqing Zhang

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  1. Cen Zhang
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  2. Shuo Li
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Correspondence to Liping Xie or Rongqing Zhang.

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Zhang, C., Li, S., Ma, Z. et al. A Novel Matrix Protein p10 from the Nacre of Pearl Oyster (Pinctada fucata) and Its Effects on Both CaCO3 Crystal Formation and Mineralogenic Cells. Mar Biotechnol 8, 624–633 (2006). https://doi.org/10.1007/s10126-006-6037-1

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