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Isolation, Characterization, and Expression Analysis of Three Actin Genes in the New Zealand Black-Footed Abalone, Haliotis iris

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Abstract

Three actin genes—H. irisA1, H. irisA2, and H. irisA3—from the mollusc Haliotis iris (New Zealand black-footed abalone) was isolated by polymerase chain reaction (PCR). The genes were similar to molluscan (84.1% to 94.9%) and vertebrate (84.5% to 86.6%) actins. The sequence similarity between the genes ranged from 88.5% to 93.2%. The greatest disparity, 32.3%, was found over a 99-nt region located at nt 808–906 of H. irisA1, corresponding to amino acids 212–244 of the three actins. The H. iris actin gene family contains at least eight members. Reverse transcription (RT)-PCR analysis of the three genes showed H. irisA1 and H. irisA2 were expressed at low levels in fertilized eggs and blastula stages and at high levels in trochophore and veliger larvae. H. irisA3 was detected in fertilized eggs; it was not detected in the blastula stages and at high levels in the trochophore and veliger larvae. The structure and expression of the three actin genes are discussed.

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Acknowledgments

Maxine Bryant was supported by a Tūāpapa Pūtaiao Fellowship and a University of Canterbury Doctoral Scholarship. The authors would like to acknowledge Pacifica Fisheries for their help in providing abalone larval samples.

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Author notes

  1. Heather J. Flint

    Present address: Forest Research, Private Bag 3020, Rotorua, New Zealand

Authors and Affiliations

  1. School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8001, New Zealand

    Maxine J. Bryant, Heather J. Flint & Frank Y. T. Sin

Authors
  1. Maxine J. Bryant
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  2. Heather J. Flint
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  3. Frank Y. T. Sin
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Correspondence to Frank Y. T. Sin.

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Bryant, M.J., Flint, H.J. & Sin, F.Y.T. Isolation, Characterization, and Expression Analysis of Three Actin Genes in the New Zealand Black-Footed Abalone, Haliotis iris. Mar Biotechnol 8, 110–119 (2006). https://doi.org/10.1007/s10126-005-5139-5

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