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Genetic Analysis of Selected Strains of Eastern Oyster (Crassostrea virginica Gmelin) Using AFLP and Microsatellite Markers

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Abstract

Amplified fragment length polymorphisms (AFLPs) and microsatellite markers were used to examine genetic variation and divergence in 4 selected strains (DBH, NEH, FMF, and CTS) and 1 wild population (DBW) of the eastern oyster Crassostrea virginica Gmelin. Eighty-six AFLP markers (from 3 primer pairs) and 5 microsatellite loci were used for the analysis of 30 oysters from each of the 5 populations. Microsatellite loci were considerably more variable than AFLPs. The observed heterozygosity ranged from 0.560 to 0.640 across populations for microsatellites, and from 0.186 to 0.207 for AFLPs. Both F st and φPT of microsatellite data and φPT statistics of AFLP data revealed significant divergence between all pairs of populations. There was no significant reduction in heterozygosity in all 4 selected strains; however, the number of alleles per locus was considerably lower in the selected strains than in the wild population. Two strains subjected to long-term selection for disease resistance shared frequency shifts at a few loci, which deserve further analysis to determine if they are linked to disease-resistance genes.

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Acknowledgments

We thank Mr. Dave Relyea, Dr. Inke Sunila, and Mr. Gregory DeBrosse for providing oyster strains or samples. This work is supported by grants from the New Jersey Commission on Science and Technology (00-2042-007-20) and Sea Grant Oyster Disease Research Program (R/OD-9915 and ODRP-29). This is publication IMCS-04-13 and NJMSC-04-567.

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Yu, Z., Guo, X. Genetic Analysis of Selected Strains of Eastern Oyster (Crassostrea virginica Gmelin) Using AFLP and Microsatellite Markers. Mar Biotechnol 6, 575–586 (2004). https://doi.org/10.1007/s10126-004-3600-5

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