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Sponge-Cell Culture? A Molecular Identification Method for Sponge Cells

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Abstract

Dissociated sponge cells are easily confused with unicellular organisms. This has been an obstacle in the development of sponge-cell lines. We developed a molecular detection method to identify cells of the sponge Dysidea avara in dissociated cell cultures. The 18S ribosomal RNA gene from a Dysidea avara specimen was sequenced and compared to eukaryotic 18S rDNA sequences picked up from a proliferating cell culture that originated from a dissociated Dysidea avara specimen. Our method proved unambiguously that this was not a sponge-cell culture. Therefore, it provides a valuable tool for further research on sponge-cell cultures.

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Authors and Affiliations

  1. Wageningen University, Food and Bioprocess Engineering Group, P.O. Box 8129, 6700 EV Wageningen, The Netherlands

    Detmer Sipkema, Ronald Osinga, Johannes Onji & René H. Wijffels

  2. Wageningen University, Laboratory of Microbiology, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands

    Hans G.H.J. Heilig & Antoon D.L. Akkermans

Authors
  1. Detmer Sipkema
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  2. Hans G.H.J. Heilig
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  3. Antoon D.L. Akkermans
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  4. Ronald Osinga
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  5. Johannes Onji
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  6. René H. Wijffels
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Correspondence to Detmer Sipkema.

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Sipkema, D., Heilig, H., Akkermans, A. et al. Sponge-Cell Culture? A Molecular Identification Method for Sponge Cells. Mar. Biotechnol. 5, 443–449 (2003). https://doi.org/10.1007/s10126-002-0090-1

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  • DOI: https://doi.org/10.1007/s10126-002-0090-1

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