Abstract
Genetic linkage maps of the guppy (Poecilia reticulata) were constructed from independent crosses between the Tuxedo strain and a feral line (Wildtype). Segregation patterns of random amplified polymorphic DNA (RAPD) markers and phenotypic markers were investigated in F2 offspring of Tuxedo ♂♂ × Wildtype ♀♀ and Wildtype ♂♂ × Tuxedo ♀♀ crosses. Among the 300 and 276 RAPD markers scored for the respective crosses, linkages were identified for 230 and 212, respectively. The Tuxedo ♂♂ × Wildtype ♀♀ and Wildtype ♂♂ × Tuxedo ♀♀ maps spanned 2100 Kosambi centiMorgans (cMK) and 1900 cMK, respectively, in 28 linkage groups. Average marker resolution was 10 cMK. Genome length was estimated at 4410 cMK and 4060 cMK for the respective crosses, with an average physical distance of 166 kbp/cMK. Several RAPD markers were closely linked to or mapped onto the loci for the sex-determining region (SdR), and the sex-linked black caudal-peduncle (Bcp) and red tail (Rdt) genes. These primary linkage maps are the initial step toward the construction of a composite high-density map to facilitate map-based cloning and marker-assisted selection of quantitative trait loci that are essential for the development of comprehensive breeding programs for the guppy.
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References
J.J. Agresti S. Seki A. Cnaani S. Poompuang E. Hallerman N. Umiel G. Hulata G.A.E. Gall B. May (2000) ArticleTitleBreeding new strains of tilapia: development of an artificial center of origin and linkage map based on AFLP and microsatellite loci. Aquaculture 185 43–56 Occurrence Handle10.1016/S0044-8486(99)00335-X Occurrence Handle1:CAS:528:DC%2BD3cXitlKrtbc%3D
R.W. Beeman S.J. Brown (1999) ArticleTitleRAPD-based genetic linkage maps of Tribolium castaneum. Genetics 153 333–338 Occurrence Handle1:CAS:528:DyaK1MXmsVWqtbs%3D Occurrence Handle10471716
K.R. Dinesh T.M. Lim K.L. Chua W.K. Chan V.P.E. Phang (1993) ArticleTitleRAPD analysis: an efficient method of DNA fingerprinting in fishes. Zool Sci 10 849–854 Occurrence Handle1:CAS:528:DyaK2cXivFSru7Y%3D Occurrence Handle7764373
K.R. Dinesh W.K. Chan T.M. Lim V.P.E. Phang (1995) ArticleTitleRAPD markers in fishes: an evaluation of resolution and reproducibility. Asia-Pac J Mol Biol Biotechnol 3 112–118
C.S. Echt C.D. Nelson (1997) ArticleTitleLinkage mapping and genome length in eastern white pine (Pinus strobus L.). Theor Appl Genet 94 0131–0137 Occurrence Handle10.1007/s001220050511
A.A. Fernando V.P.E. Phang (1990) ArticleTitleInheritance of red and blue caudal fin colorations in two domesticated varieties of the guppy, Poecilia reticulata. J Aqua Trop 5 209–217
C.L. Foo K.R. Dinesh T.M. Lim W.K. Chan V.P.E. Phang (1995) ArticleTitleInheritance of RAPD markers in the guppy fish, Poecilia reticulata. Zool Sci 12 535–541 Occurrence Handle1:CAS:528:DyaK28Xjt1Shsw%3D%3D Occurrence Handle8590830
F.J. García de León J.F. Dallas B. Chatain M. Canonne J.J. Versini F. Bonhomme (1995) ArticleTitleDevelopment and use of microsatellite markers in sea bass, Dicentrarchus labrax (Linnaeus, 1758) (Perciformes: Serranidae). Mol Mar Biol Biotechnol 4 62–68 Occurrence Handle7749467
M.A. Gates L. Kim E.S. Egan T. Cardozo H.I. Sirotkin S.T. Dougan D. Lashkari R. Abagyan A.F. Schier W.S. Talbot (1999) ArticleTitleA genetic linkage map for zebrafish: comparative analysis and localization of genes and expressed sequences. Genome Res 9 334–347 Occurrence Handle1:CAS:528:DyaK1MXivVOrtrY%3D Occurrence Handle10207156
R. Hinegardner D.E. Rosen (1972) ArticleTitleCellular DNA content and the evolution of teleostean fishes. Am Nat 106 621–644 Occurrence Handle1:CAS:528:DyaE38Xls1ahs78%3D
S.H. Hulbert T.W. Ilott E.J. Legg S.J. Lincoln E.J. Lander R.W. Michelmore (1988) ArticleTitleGenetic analysis of the fungus, Bremia lactucae, using restriction fragment polymorphisms. Genetics 120 947–958 Occurrence Handle1:STN:280:BiaC3sjptlI%3D Occurrence Handle2906309
P. Hurme O. Savolainen (1999) ArticleTitleComparison of homology and linkage of random amplified polymorphic DNA (RAPD) markers between individual trees of Scots pine (Pinus sylvestris L.). Mol Ecol 8 15–22 Occurrence Handle10.1046/j.1365-294X.1999.00532.x Occurrence Handle1:CAS:528:DyaK1MXjtVOgtbY%3D
S.L. Johnson C.N. Midson E.W. Ballinger J.H. Postlethwait (1994) ArticleTitleIdentification of RAPD primers that reveal extensive polymorphisms between laboratory strains of zebrafish. Genomics 19 152–156 Occurrence Handle10.1006/geno.1994.1026 Occurrence Handle1:CAS:528:DyaK2cXmt1eis7w%3D Occurrence Handle8188217
S. Kazianis D.C. Morizot B.B. McEntire R.S. Narin R.L. Borowsky (1996) ArticleTitleGenetic mapping in Xiphophorus hybrid fish: assignment of 43 AP-PCR/RAPD and isozyme markers to multipoint linkage groups. Genome Res 6 280–289 Occurrence Handle1:CAS:528:DyaK28XjtVeiurs%3D Occurrence Handle8723721
R.V. Kesseli I. Paran R.W. Michelmore (1994) ArticleTitleAnalysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. Genetics 136 1435–1446 Occurrence Handle1:CAS:528:DyaK2MXhtFantg%3D%3D Occurrence Handle7912217
G. Khoo T.M. Lim W.K. Chan V.P.E. Phang (1999a) ArticleTitleGenetic basis of the variegated tail pattern in the guppy, Poecilia reticulata. Zool Sci 16 431–437
G. Khoo T.M. Lim W.K. Chan V.P.E. Phang (1999b) ArticleTitleSex-linkage of the black caudal-peduncle and red tail genes in the Tuxedo strain of the guppy, Poecilia reticulata. Zool Sci 16 629–638
G. Khoo T.M. Lim W.K. Chan V.P.E. Phang (1999c) ArticleTitleLinkage analysis and mapping of three sex-linked color pattern genes in the guppy, Poecilia reticulata. Zool Sci 16 893–903 Occurrence Handle1:CAS:528:DC%2BD3cXhtlyks7k%3D
G. Khoo K.F. Lim D.K.Y. Gan F. Chen W.K. Chan T.M. Lim V.P.E. Phang (2002) ArticleTitleGenetic diversity within and among feral populations and domesticated strains of the guppy (Poecilia reticulata) in Singapore. Mar Biotechnol Occurrence Handle1:CAS:528:DC%2BD38XjtVOhu74%3D
V.S. Kirpichnikov (1981) Genetic Bases of Fish Selection. Translated by G.G. Gause. Springer-Verlag Berlin-Heidelberg, Germany
T.D. Kocher W.J. Lee H. Sobolewska D. Penman B. McAndrew (1998) ArticleTitleA genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus). Genetics 148 1225–1232 Occurrence Handle1:CAS:528:DyaK1cXks1eitL8%3D Occurrence Handle9539437
D.D. Kosambi (1944) ArticleTitleThe estimation of map distance from recombination values. Ann Eugen 12 172–175
L.S. Kumar (1999) ArticleTitleDNA markers in plant improvement. Biotechnol Adv 17 143–182 Occurrence Handle1:CAS:528:DyaK1MXmslyksL0%3D
H. Laayouni M. Santos A. Fontdevila (2000) ArticleTitleToward a physical map of Drosophila buzzatii: use of randomly amplified polymorphic DNA polymorphisms and sequence-tagged site landmarks. Genetics 156 1797–1816 Occurrence Handle1:CAS:528:DC%2BD3MXjsFGhtg%3D%3D Occurrence Handle11102375
J.-Z. Lin K. Ritland (1996) ArticleTitleConstruction of a genetic map in the wild plant Mimulus using RAPD and isozyme markers. Genome 39 63–70 Occurrence Handle1:CAS:528:DyaK28XitV2gsLg%3D
K.F. Manly J.M. Olson (1999) ArticleTitleOverview of QTL mapping software and introduction to Map Manager QT. Mamm Genome 10 327–334 Occurrence Handle10087288
M. Menotti-Raymond V.A. David L.A. Lyons A.A. Schaffer J.F. Tomlin M.K. Hutton S.J. O'Brien (1999) ArticleTitleA genetic linkage map of microsatellites in the domestic cat (Felis catus). Genomics 57 9–23 Occurrence Handle1:CAS:528:DyaK1MXitFOms7o%3D Occurrence Handle10191079
R.W. Michelmore I. Paran R.V. Kesseli (1991) ArticleTitleIdentification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88 9828–9832 Occurrence Handle1682921
S.S. Moore V. Whan G.P. Davis K. Byrne D.J.S. Hetzel N. Preston (1999) ArticleTitleThe development and application of genetic markers for the Kuruma prawn Penaeus japonicus. Aquaculture 173 19–32 Occurrence Handle10.1016/S0044-8486(98)00461-X Occurrence Handle1:CAS:528:DyaK1MXhvVGhsL4%3D
M.C. Moretzsohn C.D.M. Nunes M.E. Ferreira D. Grattapaglia (2000) ArticleTitleRAPD linkage mapping of the shell thickness locus in oil palm (Elaeis guineensis Jacq.). Theor Appl Genet 100 63–70 Occurrence Handle1:CAS:528:DC%2BD3cXht1aqtrg%3D
D.C. Morizot S.A. Slaugenhaupt K.D. Kallman A. Chakravarti (1991) ArticleTitleGenetic linkage map of fishes of the genus Xiphophorus (Teleostei: Poeciliidae). Genetics 127 399–410 Occurrence Handle1:STN:280:By6C28bivFQ%3D Occurrence Handle2004711
I. Nanda M. Schartl J.T. Epplen W. Feichtinger M. Schmid (1993) ArticleTitlePrimitive sex chromosomes in poeciliid fishes harbor simple repetitive DNA sequences. J Exp Zool 265 301–308 Occurrence Handle1:CAS:528:DyaK3sXhvVyis74%3D Occurrence Handle8436922
K. Naruse S. Fukamachi H. Mitani M. Kondo T. Matsuoka S. Kondo N. Hanamura Y. Morita K. Hasegawa R. Nishigaki A. Shimada H. Wada T. Kusakabe N. Suzuki M. Kinoshita A. Kanamori T. Terado H. Kimura M. Nonaka A. Shima (2000) ArticleTitleA detailed linkage map of medaka, Oryzias latipes: comparative genomics and genome evolution. Genetics 154 1773–1784 Occurrence Handle10747068
M. Ohtsuka S. Makino Y. Yoda H. Wada K. Naruse H. Mitani A. Shima K. Ozato M. Kimura H. Inoko (1999) ArticleTitleConstruction of a linkage map of the medaka (Oryzias latipes) and mapping of the Da mutant locus defective in dorsoventral patterning. Genome Res 9 1277–1287 Occurrence Handle10.1101/gr.9.12.1277 Occurrence Handle1:CAS:528:DC%2BD3cXivV2qsg%3D%3D Occurrence Handle10613850
I. Paran R.W. Michelmore (1993) ArticleTitleDevelopment of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85 985–993 Occurrence Handle1:CAS:528:DyaK3sXktVWru70%3D
V.P.E. Phang A.A. Fernando (1991) ArticleTitleLinkage analysis of the X-linked green tail and blue tail color genes in the guppy, Poecilia reticulata. Zool Sci 8 975–981
V.P.E. Phang L.N. Ng A.A. Fernando (1989) ArticleTitleInheritance of the snakeskin color pattern in the guppy, Poecilia reticulata. J Hered 80 393–399 Occurrence Handle1:STN:280:By%2BD3M%2FgsVw%3D Occurrence Handle2794473
V.P.E. Phang A.A. Fernando E.W.K. Chia (1990) ArticleTitleInheritance of the color patterns of the blue snakeskin and red snakeskin varieties of the guppy, Poecilia reticulata. Zool Sci 7 419–425
V.P.E. Phang G. Khoo S.P. Ang (1999) ArticleTitleInteraction between the autosomal recessive bar gene and the Y-linked snakeskin body (Ssb) pattern gene in the guppy, Poecilia reticulata. Zool Sci 16 905–908
J.H. Postlethwait S.L. Johnson C.N. Midson W.S. Talbot M. Gates E.W. Ballinger D. Africa R. Andrews T. Carl J.S. Eisen S. Horne C.B. Kimmel M. Hutchinson M. Jonhson A. Rodriguez (1994) ArticleTitleA genetic linkage map for the zebrafish. Science 264 699–703 Occurrence Handle1:CAS:528:DyaK2cXktVagsLo%3D Occurrence Handle8171321
W.S. Reiter J.G.K. Williams K.A. Feldmann J.A. Rafalski S.V. Tingey P.A. Scolnik (1992) ArticleTitleGlobal and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs. Proc Natl Acad Sci USA 89 1477–1481 Occurrence Handle1:CAS:528:DyaK38Xhs1Oltr0%3D Occurrence Handle1346933
L.H. Rieseberg (1996) ArticleTitleHomology among RAPD fragments in interspecific comparisons. Mol Ecol 5 99–105 Occurrence Handle1:CAS:528:DyaK28Xit12hurY%3D
T. Sakamoto R.G. Danzmann K. Gharbi P. Howard A. Ozaki S.K. Khoo R.A. Woram N. Okamoto M.M. Ferguson L.E. Holm R. Guyomard B. Hoyheim (2000) ArticleTitleA microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics 155 1331–1345 Occurrence Handle1:CAS:528:DC%2BD3cXlsFSku7Y%3D Occurrence Handle10880492
H. Wada K. Naruse A. Shimada A. Shima (1995) ArticleTitleGenetic linkage map of a fish, the Japanese medaka Oryzias latipes. Mol Mar Biol Biotechnol 4 269–274 Occurrence Handle1:CAS:528:DyaK2MXnvVOrs7w%3D Occurrence Handle7670603
J. Welsh M. McClelland (1990) ArticleTitleFingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18 7213–7218 Occurrence Handle2259619
J.G.K. Williams A.R. Kubelik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18 6531–6535 Occurrence Handle1979162
Ö. Winge (1927) ArticleTitleThe location of eighteen genes in Lebistes reticulatus. J Genet 18 1–43
Y. Yasukochi (1998) ArticleTitleA dense genetic map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics 150 1513–1525 Occurrence Handle1:CAS:528:DyaK1MXitVelsQ%3D%3D Occurrence Handle9832528
F. Yates (1934) ArticleTitleContingency tables involving small numbers and the χ2 test. J R Stat Soc (Suppl) 1 217–235
Acknowledgements
This project was funded by research grants from the European Commission (CI1*-CT94-0021) and National University of Singapore (R-154-000-040-112) to V.P.E. Phang (P.I.), T.M. Lim, and W.-K. Chan.
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Khoo, G., Lim, M., Suresh, H. et al. Genetic Linkage Maps of the Guppy (Poecilia reticulata): Assignment of RAPD Markers to Multipoint Linkage Groups . Mar. Biotechnol. 5, 279–293 (2003). https://doi.org/10.1007/s10126-002-0072-3
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DOI: https://doi.org/10.1007/s10126-002-0072-3