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Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy

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Abstract

This study has evaluated the effects of photodynamic inactivation (PDI) using erythrosine as photosensitizer and green light-emitting diode (LED) on biofilms of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans. We have also evaluated the effect of sucrose on biofilm formation and bacterial growth and sensitivity to PDI. Biofilms were formed in suspension of 106 cells/ml on plates before being grown in broth culture with and without sucrose and incubated for 48 h. Next, the treatment was applied using erythrosine at a concentration of 400 μM for 5 min and green LED (532 ± 10 nm) for 3 min on biofilms alone and in combination. The plates were washed and sonicated to disperse the biofilms, and serial dilutions were carried and aliquots seeded in Sabouraud agar before incubation for 48 h. Next, the colony-forming units per milliliter (CFU/ml; log10) were counted and analyzed statistically (ANOVA, Tukey test, P ≤ 0.05). Results show that S. mutans favors the growth of C. albicans in biofilms with sucrose, with treatment not being effective. However, when the biofilm was grown without sucrose, we found a reduction in biofilm formation and a significant decrease in the PDI treatment (P < 0.0001). In conclusion, both growth and sensitivity to PDI in biofilms of C. albicans are strongly influenced by bacterial combination, and the presence of sucrose affected directly the growth and sensitivity of the biofilm to PDI as sucrose is the substrate for construction of the exopolysaccharide matrix.

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Acknowledgements

The authors would like to thank the State of São Paulo University (UNESP) for the resources and encouragement of this research.

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Authors and Affiliations

  1. Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, São José dos Campos, SP, 12245-000, Brazil

    Fernanda Malagutti Tomé, Lucas De Paula Ramos, Fernanda Freire, Cristiane Aparecida Pereira, Ingrid Christine Barbosa de Oliveira, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge & Luciane Dias de Oliveira

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  1. Fernanda Malagutti Tomé
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  2. Lucas De Paula Ramos
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  3. Fernanda Freire
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  4. Cristiane Aparecida Pereira
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  5. Ingrid Christine Barbosa de Oliveira
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  6. Juliana Campos Junqueira
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  7. Antonio Olavo Cardoso Jorge
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  8. Luciane Dias de Oliveira
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Correspondence to Fernanda Malagutti Tomé.

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The authors declare that they did not receive funding for the development of this research.

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All ethical procedures were followed. Because the research used ATCC strains of microorganisms and did not involve any kinds of animal, the approval committee of ethics and informed consent do not apply to work.

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Tomé, F.M., Paula Ramos, L.D., Freire, F. et al. Influence of sucrose on growth and sensitivity of Candida albicans alone and in combination with Enterococcus faecalis and Streptococcus mutans to photodynamic therapy. Lasers Med Sci 32, 1237–1243 (2017). https://doi.org/10.1007/s10103-017-2201-2

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  • DOI: https://doi.org/10.1007/s10103-017-2201-2

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