Skip to main content

Advertisement

SpringerLink
Log in

Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

Modulation of cytochrome c oxidase activity has been pointed as a possible key mechanism for low-level laser therapy (LLLT) in unhealthy biological tissues. But recent studies by our research group with LLLT in healthy muscles before exercise found delayed skeletal muscle fatigue development and improved biochemical status in muscle tissue. Therefore, the aim of this study was to evaluate effects of different LLLT doses and wavelengths in cytochrome c oxidase activity in intact skeletal muscle. In this animal experiment, we irradiated the tibialis anterior muscle of rats with three different LLLT doses (1, 3, and 10 J) and wavelengths (660, 830, and 905 nm) with 50 mW power output. After irradiation, the analyses of cytochrome c oxidase expression by immunohistochemistry were analyzed at 5, 10, 30 min and at 1, 2, 12, and 24 h. Our results show that LLLT increased (p < 0.05) cytochrome c oxidase expression mainly with the following wavelengths and doses: 660 nm with 1 J, 830 nm with 3 J, and 905 nm with 1 J at all time points. We conclude that LLLT can increase cytochrome c oxidase activity in intact skeletal muscle and that it contributes to our understanding of how LLLT can enhance performance and protect skeletal muscles against fatigue development and tissue damage. Our findings also lead us to think that the combined use of different wavelengths at the same time can enhance LLLT effects in skeletal muscle performance and other conditions, and it can represent a therapeutic advantage in clinical settings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Bjordal JM, Lopes-Martins RA, Iversen VV (2006) A randomised, placebo controlled trial of low level laser therapy for activated Achilles tendinitis with microdialysis measurement of peritendinous prostaglandin E2 concentrations. Br J Sports Med 40:76–80

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Chow RT, Heller GZ, Barnsley L (2006) The effect of 300 mW, 830 nm laser on chronic neck pain: a double-blind, randomized, placebo-controlled study. Pain 124:201–210

    Article  PubMed  Google Scholar 

  3. Chow RT, Johnson MI, Lopes-Martins RA, Bjordal JM (2009) Efficacy of low-level laser therapy in the management of neck pain: a systematic review and meta-analysis of randomised placebo or active-treatment controlled trials. Lancet 374:1897–1908

    Article  PubMed  Google Scholar 

  4. Huang YY, Chen AC, Carrol JD, Hamblim MR (2009) Biphasic dose response in low level light therapy. Dose Response 7:358–383

    Article  PubMed Central  PubMed  Google Scholar 

  5. Goldman JA, Chiapella J, Casey H, Bass N, Graham J, McClatchey W, Dronavalli RV, Brown R, Bennett WJ, Miller SB, Wilson CH, Pearson B, Haun C, Persinski L, Huey H, Muckerheide M (1980) Laser therapy of rheumatoid arthritis. Lasers Surg Med 1:93–101

    Article  CAS  PubMed  Google Scholar 

  6. Hegedus B, Viharos L, Gervain M, Gálfi M (2009) The effect of low-level laser in knee osteoarthritis: a double-blind, randomized, placebo-controlled trial. Photomed Laser Surg 27:577–584

    Article  PubMed Central  PubMed  Google Scholar 

  7. Stergioulas A, Stergioula M, Aarskog R, Lopes-Martins RA, Bjordal JM (2008) Effects of low-level laser therapy and eccentric exercises in the treatment of recreational athletes with chronic Achilles tendinopathy. Am J Sports Med 36:881–887

    Article  PubMed  Google Scholar 

  8. Ozcelik O, Cenk Haytac M, Kunin A, Seydaoglu G (2008) Improved wound healing by low-level laser irradiation after gingivectomy operations: a controlled clinical pilot study. J Clin Periodontol 35:250–254

    Article  PubMed  Google Scholar 

  9. Schubert MM, Eduardo FP, Guthrie KA, Franquin JC, Bensadoun RJ, Migliorati CA, Lloid CM, Eduardo CP, Walter NF, Marques MM, Hamdi M (2007) A phase III randomized double-blind placebo-controlled clinical trial to determine the efficacy of low level laser therapy for the prevention of oral mucositis in patients undergoing hematopoietic cell transplantation. Support Care Cancer 15:1145–1154

    Article  PubMed  Google Scholar 

  10. Basford JR, Sheffield CG, Harmsen WS (1999) Laser therapy: a randomized, controlled trial of the effects of low-intensity Nd:YAG laser irradiation on musculoskeletal back pain. Arch Phys Med Rehabil 80:647–652

    Article  CAS  PubMed  Google Scholar 

  11. Gur A, Sarac AJ, Cevik R, Altindag O, Sarac S (2004) Efficacy of 904 nm gallium arsenide low level laser therapy in the management of chronic myofascial pain in the neck: a double-blind and randomize-controlled trial. Lasers Surg Med 35:229–235

    Article  PubMed  Google Scholar 

  12. Rochkind S, Leider-Trejo L, Nissan M, Shamir MH, Kharenko O, Alon M (2007) Efficacy of 780-nm laser phototherapy on peripheral nerve regeneration after neurotube reconstruction procedure (double-blind randomized study). Photomed Laser Surg 25:137–143

    Article  PubMed  Google Scholar 

  13. Lampl Y, Zivin JA, Fisher M, Lew R, Welin L, Dahlof B, Borenstein P, Andersson B, Perez J, Caparo C, Ilic S, Oron U (2007) Infrared laser therapy for ischemic stroke: a new treatment strategy. Results of the neurothera effectiveness and safety trial-1 (NEST-1). Stroke 38:1843–1849

    Article  PubMed  Google Scholar 

  14. Leal Junior EC, Lopes-Martins RA, de Almeida P, Ramos L, Iversen VV, Bjordal JM (2010) Effect of low-level laser therapy (GaAs 904 nm) in skeletal muscle fatigue and biochemical markers of muscle damage in rats. Eur J Appl Physiol 108:1083–1088

    Article  PubMed  Google Scholar 

  15. Leal Junior EC, Lopes-Martins RA, Dalan F, Ferrari M, Sbabo FM, Generosi RA, Baroni BM, Penna SC, Iversen VV, Bjordal JM (2008) Effect of 655-nm low-level laser therapy on exercise-induced skeletal muscle fatigue in humans. Photomed Laser Surg 26:419–424

    Article  PubMed  Google Scholar 

  16. Leal Junior EC, Lopes-Martins RA, Vanin AA, Baroni BM, Grosselli D, De Marchi T, Iversen VV, Bjordal JM (2009) Effect of 830 nm low-level laser therapy in exercise-induced skeletal muscle fatigue in humans. Lasers Med Sci 24:425–431

    Article  PubMed  Google Scholar 

  17. Leal Junior EC, Lopes-Martins RA, Rossi RP, De Marchi T, Baroni BM, de Godoi V, Marcos RL, Ramos L, Bjordal JM (2009) Effect of cluster multi-diode light emitting diode therapy (LEDT) on exercise-induced skeletal muscle fatigue and skeletal muscle recovery in humans. Lasers Surg Med 41:572–577

    Article  PubMed  Google Scholar 

  18. Leal Junior EC, Lopes-Martins RA, Frigo L, De Marchi T, Rossi RP, de Godoi V, Tomazoni SS, da Silva DP, Basso M, Lotti Filho P, Corsetti FV, Iversen VV, Bjordal JM (2010) Effects of low-level laser therapy (LLLT) in the development of exercise-induced skeletal muscle fatigue and changes in biochemical markers related to post-exercise recovery. J Orthop Sports Phys Ther 40:524–532

    Article  PubMed  Google Scholar 

  19. Leal Junior EC, Lopes-Martins RA, Baroni BM, De Marchi T, Rossi RP, Grosselli D, Generosi RA, de Godoi V, Basso M, Mancalossi JL, Bjordal JM (2009) Comparison between single-diode low-level laser therapy (LLLT) and LED multi-diode (cluster) therapy (LEDT) applications before high-intensity exercise. Photomed Laser Surg 27:617–623

    Article  PubMed  Google Scholar 

  20. Leal Junior EC, Lopes-Martins RA, Baroni BM, De Marchi T, Taufer D, Manfro DS, Rech M, Danna V, Grosselli D, Generosi RA, Marcos RL, Ramos L, Bjordal JM (2009) Effect of 830 nm low-level laser therapy applied before high-intensity exercises on skeletal muscle recovery in athletes. Lasers Med Sci 24:857–863

    Article  PubMed  Google Scholar 

  21. Hayworth CR, Rojas JC, Padilla E, Holmes GM, Sheridan EC, Gonzalez-Lima F (2010) In vivo low-level light therapy increases cytochrome oxidase in skeletal muscle. Photochem Photobiol 86:673–680

    Article  CAS  PubMed  Google Scholar 

  22. Santos LA, Marcos RL, Tomazoni SS, Vanin AA, Antonialli FC, Grandinetti VD, Albuquerque-Pontes GM, de Paiva PR, Lopes-Martins RA, de Carvalho PD, Bjordal JM, Leal-Junior EC (2014) Effects of pre-irradiation of low-level laser therapy with different doses and wavelengths in skeletal muscle performance, fatigue, and skeletal muscle damage induced by tetanic contractions in rats. Lasers Med Sci. doi:10.1007/s10103-014-1560-1

  23. Ferraresi C, Oliveira TB, Zafalon LO, Reiff RB, Baldissera V, Perez SE, Júnior EM, Parizotto NA (2011) Effects of low laser therapy (808 nm) on physical strength training in humans. Lasers Med Sci 26:349–358

    Article  PubMed  Google Scholar 

  24. Bakeeva LE, Manteifel VM, Rodichev EB, Karu TI (1993) Formation of gigantic mitochondria in human blood lymphocytes under the effects oh an He-Ne laser light. Mol Biol (Mosk) 27:608–617

    CAS  Google Scholar 

  25. Manteifel VM, Karu TI (2005) Structure of mitochondria and activity of their respiratory chain in subsequent generations of yeast cells exposed to He-Ne laser light. Izv Akad Nauk Ser Biol 6:672–683

    PubMed  Google Scholar 

  26. Rennó AC, Toma RL, Feitosa SM, Fernandes K, Bossini OS, de Oliveira P, Parizotto N, Ribeiro DA (2011) Comparative effects of low-intensity pulsed ultrasound and low-level laser therapy on injured skeletal muscle. Photomed Laser Surg 29:5–10

    Article  PubMed  Google Scholar 

  27. Silveira PC, Silva LA, Fraga DB, Freitas TP, Streck EL, Pinho R (2009) Evaluation of mitochondrial respiratory chain activity in muscle healing by low-level laser therapy. J Photochem Photobiol B 95:89–92

    Article  CAS  PubMed  Google Scholar 

  28. Leal-Junior EC, Vanin AA, Miranda EF, de Carvalho PD, Dal Corso S, Bjordal JM (2013) Effect of phototherapy (low-level laser therapy and light-emitting diode therapy) on exercise performance and markers of exercise recovery: a systematic review with meta-analysis. Lasers Med Sci [Epub ahead of print]

Download references

Acknowledgments

Professor Ernesto Cesar Pinto Leal-Junior would like to thank the São Paulo Research Foundation (FAPESP) research grant number 2010/52404-0. Professor Lucio Frigo would like to thank the FAPESP research grant number 2012/06832-5. Gianna Móes Albuquerque-Pontes would like to thank the FAPESP master degree scholarship number 2012/11385-8.

Conflict of interest

Professor Ernesto Cesar Pinto Leal-Junior receives research support from Multi Radiance Medical (Solon, OH, USA), a laser device manufacturer. The remaining authors declare that they have no conflict of interests.

Author information

Authors and Affiliations

  1. Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, São Paulo, SP, 01504-001, Brazil

    Gianna Móes Albuquerque-Pontes, Cláudia Oliveira Caires, Paulo de Tarso Camillo de Carvalho & Ernesto Cesar Pinto Leal-Junior

  2. Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, São Paulo, SP, 01504-001, Brazil

    Rodolfo de Paula Vieira, Victoria Nemeth, Adriane Aver Vanin, Larissa Aline Santos, Henrique Dantas Pinto, Paulo de Tarso Camillo de Carvalho & Ernesto Cesar Pinto Leal-Junior

  3. Postgraduate Program in Medicine, Universidade Nove de Julho (UNINOVE), Rua Vergueiro, 235, São Paulo, SP, 01504-001, Brazil

    Rodolfo de Paula Vieira

  4. Laboratory of Pharmacology and Experimental Therapeutics, Department of Pharmacology, University of São Paulo, Av. Prof. Lineu Prestes, 1524, São Paulo, SP, 05508-900, Brazil

    Shaiane Silva Tomazoni & Rodrigo Labat Marcos

  5. Physiotherapy Research Group, Department of Global Public Health, Faculty of Medicine and Dentistry, University of Bergen, Kalfarveien 31, 5020, Bergen, Norway

    Jan Magnus Bjordal

  6. Centre for Knowledge-Based Practice, Bergen University College, Moellendalsveien 6, 5009, Bergen, Norway

    Jan Magnus Bjordal

Authors
  1. Gianna Móes Albuquerque-Pontes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rodolfo de Paula Vieira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaiane Silva Tomazoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cláudia Oliveira Caires
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Victoria Nemeth
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Adriane Aver Vanin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Larissa Aline Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Henrique Dantas Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Rodrigo Labat Marcos
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jan Magnus Bjordal
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Paulo de Tarso Camillo de Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Ernesto Cesar Pinto Leal-Junior
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ernesto Cesar Pinto Leal-Junior.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Albuquerque-Pontes, G.M., Vieira, R.d.P., Tomazoni, S.S. et al. Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats. Lasers Med Sci 30, 59–66 (2015). https://doi.org/10.1007/s10103-014-1616-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-014-1616-2

Keywords

Search

Navigation