Abstract
Both high-intensity ultraviolet and narrowband ultraviolet B (NB-UVB) are important therapeutic options for vitiligo management, but high-intensity ultraviolet is more effective than NB-UVB. However, the underlying mechanisms have not been well investigated. Herein, we compare the effects of high-intensity ultraviolet and NB-UVB on the pigmentation of melanocytes derived from hair follicle-derived neural crest stem cells (HF-NCSCs) in vitro and study the underlying mechanisms. The HF-NCSCs were isolated from mouse whisker follicles. After radiation with high-intensity ultraviolet and NB-UVB, respectively, the cell viability by the CCK-8 assay showed gradual inhibitory effects in a dose-dependent manner, which has no apparent difference between the two modalities. The mRNA for melanogenesis factors such as tyrosinase and tyrp1 of the differentiated melanocytes increased significantly with high-intensity ultraviolet compared to the same dose of NB-UVB exposure. Furthermore, the expression of Mc1r was significantly increased by high-intensity ultraviolet in contrast to NB-UVB at the dosage of 0.5 J. By and large, these data suggest that high-intensity ultraviolet exhibited greater efficiency on the maturation of the melanocyte lineage differentiated from HF-NCSCs compared to NB-UVB with the same dose, which was probably due to the stronger stimulatory action of Mc1r. This may provide new insights into the different efficacies of high-intensity ultraviolet and NB-UVB in the treatment of vitiligo repigmentation.
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References
Asawanonda P, Kijluakiat J, Korkij W et al (2008) Targeted broadband ultraviolet b phototherapy produces similar responses to targeted narrowband ultraviolet B phototherapy for vitiligo: a randomized, double-blind study. Acta Derm Venereol 88:376–381
Asawanonda P, Charoenlap M, Korkij W (2006) Treatment of localized vitiligo with targeted broadband UVB phototherapy: a pilot study. Photodermatol Photoimmunol Photomed 22:133–136
Akar A, Tunca M, Koc E et al (2009) Broadband targeted UVB phototherapy for localized vitiligo: a retrospective study. Photodermatol Photoimmunol Photomed 25:161–163
Pacifico A, Leone G (2011) Photo(chemo)therapy for vitiligo. Photodermatol Photoimmunol Photomed 27:261–277
Vanscheidt W, Hunziker T (2009) Repigmentation by outer-root-sheath-derived melanocytes: proof of concept in vitiligo and leucoderma. Dermatology 218:342–343
Nishimura EK, Jordan SA, Oshima H et al (2002) Dominant role of the niche in melanocyte stem-cell fate determination. Nature 416:854–860
Nishimura EK, Granter SR, Fisher DE (2005) Mechanisms of hair graying: incomplete melanocyte stem cell maintenance in the niche. Science 307:720–724
Yu H, Fang D, Kumar SM et al (2006) Isolation of a novel population of multipotent adult stem cells from human hair follicles. Am J Pathol 168:1879–1888
Dong D, Jiang M, Xu X et al (2012) The effects of NB-UVB on the hair follicle-derived neural crest stem cells differentiating into melanocyte lineage in vitro. J Dermatol Sci 66:20–28
Tanimura S, Tadokoro Y, Inomata K et al (2011) Hair follicle stem cells provide a functional niche for melanocyte stem cells. Cell Stem Cell 8:177–187
Yamaguchi Y, Hearing VJ (2009) Physiological factors that regulate skin pigmentation. Biofactors 35:193–199
Osawa M, Egawa G, Mak SS et al (2005) Molecular characterization of melanocyte stem cells in their niche. Development 132:5589–5599
Wegner M (2005) Secrets to a healthy Sox life: lessons for melanocytes. Pigment Cell Res 18:74–85
Hou L, Arnheiter H, Pavan WJ (2006) Interspecies difference in the regulation of melanocyte development by SOX10 and MITF. Proc Natl Acad Sci U S A 103:9081–9085
Murisier F, Guichard S, Beermann F (2007) The tyrosinase enhancer is activated by Sox10 and Mitf in mouse melanocytes. Pigment Cell Res 20:173–184
Levy C, Khaled M, Fisher DE (2006) MITF: master regulator of melanocyte development and melanoma oncogene. Trends Mol Med 12:406–414
Chou WC, Takeo M, Rabbani P et al (2013) Direct migration of follicular melanocyte stem cells to the epidermis after wounding or UVB irradiation is dependent on Mc1r signaling. Nat Med 19:924–929
Swope VB, Jameson JA, McFarland KL et al (2012) Defining MC1R regulation in human melanocytes by its agonist α-melanocortin and antagonists agouti signaling protein and β-defensin 3. J Invest Dermatol 132:2255–2262
Newton RA, Roberts DW, Leonard JH et al (2007) Human melanocytes expressing MC1R variant alleles show impaired activation of multiple signaling pathways. Peptides 28:2387–2396
Maresca V, Flori E, Bellei B et al (2010) MC1R stimulation by alpha-MSH induces catalase and promotes its re-distribution to the cell periphery and dendrites. Pigment Cell Melanoma Res 23:263–275
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D. Dong and S. Chen contributed equally as co-first authors to the article.
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Dong, D., Chen, S., Zhang, X. et al. Comparison of high-intensity ultraviolet and NB-UVB on the maturation of melanocytes derived from hair follicle neural crest stem cells. Lasers Med Sci 29, 1649–1654 (2014). https://doi.org/10.1007/s10103-014-1568-6
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DOI: https://doi.org/10.1007/s10103-014-1568-6