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The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with miniplates: a histological and histomorphometric study on rabbits

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Abstracts

The aim of the present study was to assess, by light microscopy and histomorphometry, the repair of surgical fractures fixed with internal rigid fixation (IRF) treated or not with IR laser (λ780 nm, 50 mW, 4 × 4 J/cm2 = 16 J/cm2, ϕ = 0.5cm2, CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into 5 groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libidum. The fractures in groups II, III, IV, and V were fixed with miniplates. Animals in groups III and V were grafted with hydroxyapatite and GBR technique used. Animals in groups IV and V were irradiated at every other day during two weeks (4 × 4 J/cm2, 16 J/cm2 = 112 J/cm2). Observation time was that of 30 days. After animal death, specimens were taken, routinely processed to wax, cut and stained with HA and Sirius red, and used for histological assessment. The results of both analyses showed a better bone repair on all irradiated subjects especially when the biomaterial and GBR were used. In conclusion, the results of the present investigation are important clinically as they are suggestive that the association of hydroxyapatite, and laser light resulted in a positive and significant repair of complete tibial fractures treated with miniplates.

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Notes

  1. 2 mg/kg Acepran®, 0.2 % Univet S.A, Cambuci, SP, Brazil.

  2. Ketalar®, 50 mg/ml 0.4 ml/kg, Lab. Parke Davis Ltda, São Paulo, SP, Brazil.

  3. Rompum®, 20 mg/ml 0.2 ml/kg, Lab. Bayer Health Care S.A, São Paulo, SP, Brazil.

  4. Moyco Union Broach, York, PA, USA.

  5. Sistema 2.0 PROMM®, Comércio de Implantes Cirúrgicos Ltda. Porto Alegre, RS. Brazil.

  6. GenPhos® HATCP. BAUMER®, Mogi Mirim, SP, Brazil.

  7. Gen-derm®, BAUMER®, Mogi Mirim, SP, Brazil.

  8. The manufacturer calibrated the equipment before the experimentation.

  9. 4–0 polyglactin and 4–0 nylon, TRUSINTH®, Sutures India Pvt Ltda. Bangalore, Karnataka, India.

  10. Pentabiótico®, peniciline, streptomicine, 20.000 UI 0.2 ml/kg IM, Lab.Forte Dogde Saúde Animal Ltda, Campinas, SP, Brazil .and Banamine®, flunixin meglumine, 10 mg/ml, 0.1 ml/kg IM, Intervet Shering-Plough Animal Health, Cruzeiro, SP, Brazil.

  11. 11 Bueler®, Isomet TM1000; Markham, Ontario, Canada .

  12. AxioStar®, Zeiss, Germany.

  13. Belo Horizonte, MG, Brazil.

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Acknowledgment

The authors gratefully acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support and PhD Grant.

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No competing financial interests exist.

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Authors and Affiliations

  1. Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil

    Antonio L. B. Pinheiro, Gilberth Tadeu Santos Aciole, Thais Andrade Ramos, Tayná Assunção Gonzalez, Laís Nogueira da Silva, Luiz G. Pinheiro Soares & Jouber Mateus Santos Aciole

  2. Universidade Camilo Castelo Branco, São José dos Campos, SP, Brazil, 12245-230

    Antonio L. B. Pinheiro

  3. Instituto Nacional de Ciência e Tecnologia de Óptica e Fotônica, São Carlos, SP, Brazil, 13560-970

    Antonio L. B. Pinheiro

  4. Laboratory of Surgical Pathology, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil

    Jean Nunes dos Santos

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  1. Antonio L. B. Pinheiro
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  2. Gilberth Tadeu Santos Aciole
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  3. Thais Andrade Ramos
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  4. Tayná Assunção Gonzalez
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  5. Laís Nogueira da Silva
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  6. Luiz G. Pinheiro Soares
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  7. Jouber Mateus Santos Aciole
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  8. Jean Nunes dos Santos
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Correspondence to Antonio L. B. Pinheiro.

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Pinheiro, A.L.B., Aciole, G.T.S., Ramos, T.A. et al. The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with miniplates: a histological and histomorphometric study on rabbits. Lasers Med Sci 29, 279–288 (2014). https://doi.org/10.1007/s10103-013-1339-9

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  • DOI: https://doi.org/10.1007/s10103-013-1339-9

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