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Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair

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Abstract

Low-level laser irradiation (LLLI) and recombinant human bone morphogenetic protein type 2 (rhBMP-2) have been used to stimulate bone formation. LLLI stimulates proliferation of osteoblast precursor cells and cell differentiation and rhBMP-2 recruits osteoprogenitor cells to the bone healing area. This in vivo study evaluated the effects of LLLI and rhBMP-2 on the bone healing process in rats. Critical bone defects were created in the parietal bone in 42 animals, and the animals were divided into six treatment groups: (1) laser, (2) 7 μg of rhBMP-2, (3) laser and 7 μg of rhBMP-2, (4) 7 μg of rhBMP-2/monoolein gel, (5) laser and 7 μg rhBMP-2/monoolein gel, and (6) critical bone defect controls. A gallium-aluminum-arsenide diode laser was used (wavelength 780 nm, output power 60 mW, beam area 0.04 cm2, irradiation time 80 s, energy density 120 J/cm2, irradiance 1.5 W/cm2). After 15 days, the calvarial tissues were removed for histomorphometric analysis. Group 3 defects showed higher amounts of newly formed bone (37.89%) than the defects of all the other groups (P < 0.05). The amounts of new bone in defects of groups 1 and 4 were not significantly different from each other (24.00% and 24.75%, respectively), but were significantly different from the amounts in the other groups (P < 0.05). The amounts of new bone in the defects of groups 2 and 5 were not significantly different from each other (31.42% and 31.96%, respectively), but were significantly different from the amounts in the other groups (P < 0.05). Group 6 defects had 14.10% new bone formation, and this was significantly different from the amounts in the other groups (P < 0.05). It can be concluded that LLLI administered during surgery effectively accelerated healing of critical bone defects filled with pure rhBMP-2, achieving a better result than LLLI alone or the use of rhBMP-2 alone.

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Acknowledgments

We acknowledge the support of grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP - 2008/51480-4), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Pró-Reitoria de Pesquisa da Universidade de São Paulo.

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Authors and Affiliations

  1. Department of Morphology, Stomatology and Physiology, School of Dentistry at Ribeirão Preto, University of São Paulo, Avenida do Café, s/n - Bairro - Monte Alegre, 14040-904, Ribeirão Preto, São Paulo, Brazil

    Anderson Paim Rosa, Luiz Gustavo de Sousa, Simone Cecilio Hallak Regalo, João Paulo Mardegan Issa, Ana Paula Amorim Barbosa, Dimitrius Leonardo Pitol, Richard Honorato de Oliveira, Paulo Batista de Vasconcelos, Fernando José Dias, Daniela Thomazatti Chimello & Selma Siéssere

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  1. Anderson Paim Rosa
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  2. Luiz Gustavo de Sousa
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Correspondence to Selma Siéssere.

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Rosa, A.P., de Sousa, L.G., Regalo, S.C.H. et al. Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair. Lasers Med Sci 27, 971–977 (2012). https://doi.org/10.1007/s10103-011-1022-y

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