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Effects of LED phototherapy on bone defects grafted with MTA, bone morphogenetic proteins and guided bone regeneration: a Raman spectroscopic study

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Abstract

We studied peaks of calcium hydroxyapatite (CHA) and protein and lipid CH groups in defects grafted with mineral trioxide aggregate (MTA) treated or not with LED irradiation, bone morphogenetic proteins and guided bone regeneration. A total of 90 rats were divided into ten groups each of which was subdivided into three subgroups (evaluated at 15, 21 and 30 days after surgery). Defects were irradiated with LED light (wavelength 850 ± 10 nm) at 48-h intervals for 15 days. Raman readings were taken at the surface of the defects. There were no statistically significant differences in the CHA peaks among the nonirradiated defects at any of the experimental time-points. On the other hand, there were significant differences between the defects filled with blood clot and the irradiated defects at all time-points (p < 0.001, p = 0.02, p < 0.001). There were significant differences between the mean peak CHA in nonirradiated defects at all the experimental time-points (p < 0.01). The mean peak of the defects filled with blood clot was significantly different from that of the defects filled with MTA (p < 0.001). There were significant differences between the defects filled with blood clot and the irradiated defects (p < 0.001). The results of this study using Raman spectral analysis indicate that infrared LED light irradiation improves the deposition of CHA in healing bone grafted or not with MTA.

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Acknowledgments

We would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing financial support for this project.

Conflicts of interest

The authors received a grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a government research agency, but have full control of all primary data and agree to allow the journal to review their data if requested.

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Authors and Affiliations

  1. Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil

    Antonio L. B. Pinheiro, Luiz G. P. Soares, Nicole R. S. Santos & Artur Felipe S. Barbosa

  2. Universidade Camilo Castelo Branco Núcleo do Parque Tecnológico de São José dos Campos, São José dos Campos, SP, Brazil

    Antonio L. B. Pinheiro & Landulfo Silveira Júnior

  3. Instituto Nacional de Ciência e Tecnologia de Óptica e Fotônica, São Carlos, SP, Brazil

    Antonio L. B. Pinheiro

  4. Oral Epidemiology and Public Health, School of Dentistry, Federal University of Bahia, Av. Araujo Pinho, 62, Canela, Salvador, BA, Brazil

    Maria Cristina T. Cangussú

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  1. Antonio L. B. Pinheiro
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Correspondence to Antonio L. B. Pinheiro.

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Pinheiro, A.L.B., Soares, L.G.P., Cangussú, M.C.T. et al. Effects of LED phototherapy on bone defects grafted with MTA, bone morphogenetic proteins and guided bone regeneration: a Raman spectroscopic study. Lasers Med Sci 27, 903–916 (2012). https://doi.org/10.1007/s10103-011-1010-2

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