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Laser light influences cellular viability and proliferation in diabetic-wounded fibroblast cells in a dose- and wavelength-dependent manner

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Abstract

Phototherapy stimulates metabolic processes in healing wounds. Despite worldwide interest, phototherapy is not firmly established or practiced in South Africa. This study aimed to determine which dose and wavelength would better induce healing in vitro. Diabetic-induced wounded fibroblasts were irradiated with 5 or 16 J/cm2 at 632.8, 830, or 1,064 nm. Cellular morphology, viability (Trypan blue and apoptosis), and proliferation (basic fibroblast growth factor) were then determined. Cells irradiated with 5 J/cm2 at 632.8 nm showed complete wound closure and an increase in viability and basic fibroblast growth factor (bFGF) expression. Cells irradiated at 830 nm showed incomplete wound closure and an increase in bFGF expression. Cells irradiated at 1,064 nm showed incomplete closure and increased apoptosis. All cells irradiated with 16 J/cm2 at all three wavelengths showed incomplete wound closure, increased apoptosis, and decreased bFGF expression. This study showed that diabetic-wounded cells respond in a dose- and a wavelength-dependent manner to laser light. Cells responded the best when irradiated with a fluence of 5 J/cm2 at a wavelength of 632.8 nm.

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Acknowledgments

The National Laser Center (NLC; contract grant sponsor, research grant), South Africa; National Research Foundation (NRF), South Africa; Medical Research Council (MRC), South Africa and the University of Johannesburg Research Council (URC; contract grant sponsor, research grant) supported this research. Lasers were supplied by the NLC.

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  1. Laser Research Group, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, 2028, South Africa

    N. N. Houreld & H. Abrahamse

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  1. N. N. Houreld
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Correspondence to H. Abrahamse.

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Houreld, N.N., Abrahamse, H. Laser light influences cellular viability and proliferation in diabetic-wounded fibroblast cells in a dose- and wavelength-dependent manner. Lasers Med Sci 23, 11–18 (2008). https://doi.org/10.1007/s10103-007-0445-y

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