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Outbreak of NDM-1-producing Klebsiella pneumoniae ST76 and ST37 isolates in neonates

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Abstract

The purpose of this study was to investigate the epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) in Shanghai Children’s Hospital in China. Twenty-two non-duplicate CRKP strains were collected from pediatric patients between March and June in 2014. Antimicrobial susceptibility testing was conducted by the agar dilution method. Beta-lactamases were characterized by polymerase chain reaction (PCR) and DNA sequencing. The transferability of bla NDM-1 was investigated by conjugation experiment. The plasmids bearing antibiotic resistance genes were characterized by S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern hybridization. Clonal relatedness was evaluated by PFGE and multilocus sequence typing (MLST). The clinical data of patients were retrospectively reviewed. The 22 CRKP strains were resistant to most of the antimicrobial agents tested, except tigecycline and colistin. Overall, 59, 77, and 100 % of these strains were resistant to imipenem, meropenem, and ertapenem, respectively. The bla NDM-1 was positive in 77.3 % (17/22) of the CRKP strains, of which the 16 isolates from inpatients were designated as ST37 (n = 9) and ST76 (n =7) and one isolate from an outpatient belonged to ST846. The 17 bla NDM-1-positive isolates belonged to PFGE type A (n = 9), type C (n = 7), or type B (n = 1). The plasmids bearing bla NDM-1 could be transferred into recipient Escherichia coli J53 through conjugation in 88.2 % (15/17) of the strains. The hybridization results showed that the plasmids carrying the bla NDM-1 gene were approximately 50–240 kb in size. This is the first report of an outbreak caused by NDM-1-producing K. pneumoniae ST76 and ST37 among neonates.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant no. 81273559), the Shanghai Municipal Commission of Health and Family Planning (grant no. 20124026), the National Major Scientific and Technological Special Project for “Significant New Drugs Development” (grant no. 2014ZX09507009-027), and the Zhejiang Provincial Natural Science Foundation of China (grant no. Y12H190008). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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    Authors and Affiliations

    1. Department of Laboratory Medicine, Shanghai Children’s Hospital, Shanghai Jiao Tong University, Lane 24, 1400 W. Beijing Rd., Shanghai, 200040, People’s Republic of China

      J. Zhu & H. Zhang

    2. Institute of Antibiotics, Huashan Hospital, Fudan University, 12 M. Wulumuqi Rd., Shanghai, 200040, People’s Republic of China

      L. Sun, B. Ding, Y. Yang, X. Xu, D. Zhu, F. Yang & F. Hu

    3. Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, China

      L. Sun, B. Ding, Y. Yang, X. Xu, D. Zhu, F. Yang & F. Hu

    4. Department of Laboratory Medicine, Lishui People’s Hospital, Lishui, Zhejiang Province, China

      W. Liu

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    1. J. Zhu
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    J. Zhu and L. Sun contributed equally to this work.

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    Zhu, J., Sun, L., Ding, B. et al. Outbreak of NDM-1-producing Klebsiella pneumoniae ST76 and ST37 isolates in neonates. Eur J Clin Microbiol Infect Dis 35, 611–618 (2016). https://doi.org/10.1007/s10096-016-2578-z

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    • DOI: https://doi.org/10.1007/s10096-016-2578-z

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