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Fabric-skin models to assess infection transfer for impetigo contagiosa in a kindergarten scenario

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Abstract

Children in community bodies like kindergartens are predisposed to suffer from impetigo. To consider important measures for infection prevention, direct and indirect transmission routes of pathogens must be revealed. Therefore, we studied the role of skin and fabrics in the spread of the impetigo pathogen Staphylococcus aureus and the strain Streptococcus equi (surrogate to Streptococcus pyogenes) in order to assess infection transfer in realistic scenarios. The transmission of test strains was studied with standardized fabric-skin models using a technical artificial skin and fabrics of different fiber types commonly occurring in German kindergartens. In synthetic pus, both test strains persisted on artificial skin and fabrics for at least 4 h. Friction enhanced transfer, depending on the fiber type or fabric construction. In a skin-to-skin setup, the total transfer was higher than via fabrics and no decrease in the transmission rates from donor to recipients could be observed after successive direct skin contacts. Children in kindergartens may be at risk of transmission for impetigo pathogens, especially via direct skin contact, but also by the joint use of fabrics, like towels or handicraft materials. Fabric-skin models used in this study enable further insight into the transmission factors for skin infections on the basis of a practical approach.

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References

  1. Wannamaker LW (1970) Differences between streptococcal infections of the throat and of the skin. I. N Engl J Med 282(1):23–31. doi:10.1056/NEJM197001012820106

    Article  CAS  PubMed  Google Scholar 

  2. Empinotti JC, Uyeda H, Ruaro RT, Galhardo AP, Bonatto DC (2012) Pyodermitis. An Bras Dermatol 87(2):277–284

    PubMed  Google Scholar 

  3. Kristensen JK (1991) Scabies and Pyoderma in Lilongwe, Malawi. Prevalence and seasonal fluctuation. Int J Dermatol 30(10):699–702

    Article  CAS  PubMed  Google Scholar 

  4. Loffeld A, Davies P, Lewis A, Moss C (2005) Seasonal occurrence of impetigo: a retrospective 8-year review (1996–2003). Clin Exp Dermatol 30(5):512–514. doi:10.1111/j.1365-2230.2005.01847.x

    Article  CAS  PubMed  Google Scholar 

  5. Kakar N, Kumar V, Mehta G, Sharma RC, Koranne RV (1999) Clinico-bacteriological study of pyodermas in children. J Dermatol 26(5):288–293

    Article  CAS  PubMed  Google Scholar 

  6. Feldmeier H, Singh Chhatwal G, Guerra H (2005) Pyoderma, group A streptococci and parasitic skin diseases—a dangerous relationship. Trop Med Int Health 10(8):713–716. doi:10.1111/j.1365-3156.2005.01457.x

    Article  PubMed  Google Scholar 

  7. Crocker HR (1881) On the contagium of impetigo contagiosa. Lancet 117(3012):821

    Article  Google Scholar 

  8. Parker MT, Tomlinson AJ, Williams RE (1955) Impetigo contagiosa; the association of certain types of Staphylococcus aureus and of Streptococcus pyogenes with superficial skin infections. J Hyg (Lond) 53(4):458–473

    Article  CAS  Google Scholar 

  9. Brown J, Shriner DL, Schwartz RA, Janniger CK (2003) Impetigo: an update. Int J Dermatol 42(4):251–255

    Article  PubMed  Google Scholar 

  10. Barrow GI (1955) Clinical and bacteriological aspects of impetigo contagiosa. J Hyg (Lond) 53(4):495–508

    Article  CAS  Google Scholar 

  11. Darmstadt GL, Lane AT (1994) Impetigo: an overview. Pediatr Dermatol 11(4):293–303

    Article  CAS  PubMed  Google Scholar 

  12. Cruickshank JG, Lightfoot NF, Sugars KH, Colman G, Simmons MD, Tolliday J, Oakley EH (1982) A large outbreak of streptococcal pyoderma in a military training establishment. J Hyg (Lond) 89(1):9–21

    Article  CAS  Google Scholar 

  13. Ludlam H, Cookson B (1986) Scrum kidney: epidemic pyoderma caused by a nephritogenic Streptococcus pyogenes in a rugby team. Lancet 2(8502):331–333

    Article  CAS  PubMed  Google Scholar 

  14. Levy JA (2004) Common bacterial dermatoses: protecting competitive athletes. Phys Sportsmed 32(6):33–39. doi:10.3810/psm.2004.06.380

    Article  PubMed  Google Scholar 

  15. Sheehan HL, Girdwood Fergusson A (1943) Impetigo aetiology and treatment. Lancet 241(6244):547–550

    Article  Google Scholar 

  16. Chiller K, Selkin BA, Murakawa GJ (2001) Skin microflora and bacterial infections of the skin. J Investig Dermatol Symp Proc 6:170–174. doi:10.1046/j.0022-202x.2001.00043.x

    Article  CAS  PubMed  Google Scholar 

  17. Sattar SA, Springthorpe S, Mani S, Gallant M, Nair RC, Scott E, Kain J (2001) Transfer of bacteria from fabrics to hands and other fabrics: development and application of a quantitative method using Staphylococcus aureus as a model. J Appl Microbiol 90(6):962–970

    Article  CAS  PubMed  Google Scholar 

  18. Bloomfield S, Exner M, Fara GM, Scott EA (2008) Prevention of the spread of infection—the need for a family-centred approach to hygiene promotion. Euro Surveill 13(22):1–4

    Google Scholar 

  19. Waller AS, Paillot R, Timoney JF (2011) Streptococcus equi: a pathogen restricted to one host. J Med Microbiol 60(Pt 9):1231–1240. doi:10.1099/jmm.0.028233-0

    Article  CAS  PubMed  Google Scholar 

  20. Burkhardt F (1992) Mikrobiologische Diagnostik, vol 1. Auflage, Stuttgart

    Google Scholar 

  21. von Gorup-Besànez EF (1862) Lehrbuch der physiologischen Chemie. Druck und Verlag von Friedrich Vieweg und Sohn, Braunschweig

    Google Scholar 

  22. König C, Simmen HP, Blaser J (1998) Bacterial concentrations in pus and infected peritoneal fluid—implications for bactericidal activity of antibiotics. J Antimicrob Chemother 42(2):227–232

    Article  PubMed  Google Scholar 

  23. ISO 105-E04:2013 Textiles—Tests for colour fastness—Part E04: Colour fastness to perspiration; German version EN ISO 105-E04:2013

  24. DIN 53923:1978-01 Testing of textiles; determination of water absorption of textile fabrics

  25. Hammer TR, Berner-Dannenmann N, Hoefer D (2013) Quantitative and sensory evaluation of malodour retention of fibre types by use of artificial skin, sweat and radiolabelled isovaleric acid. Flavour Fragr J 28(4):238–244. doi:10.1002/ffj.3134

    Article  CAS  Google Scholar 

  26. Gwaltney JM Jr, Moskalski PB, Hendley JO (1978) Hand-to-hand transmission of rhinovirus colds. Ann Intern Med 88(4):463–467

    Article  PubMed  Google Scholar 

  27. Ghareeb PA, Bourlai T, Dutton W, McClellan WT (2013) Reducing pathogen transmission in a hospital setting. Handshake versus fist bump: a pilot study. J Hosp Infect 85(4):321–323. doi:10.1016/j.jhin.2013.08.010

    Article  CAS  PubMed  Google Scholar 

  28. Lingaas E, Fagernes M (2009) Development of a method to measure bacterial transfer from hands. J Hosp Infect 72(1):43–49. doi:10.1016/j.jhin.2009.01.022

    Article  CAS  PubMed  Google Scholar 

  29. Mbithi JN, Springthorpe VS, Boulet JR, Sattar SA (1992) Survival of hepatitis A virus on human hands and its transfer on contact with animate and inanimate surfaces. J Clin Microbiol 30(4):757–763

    PubMed Central  CAS  PubMed  Google Scholar 

  30. Jabbar U, Leischner J, Kasper D, Gerber R, Sambol SP, Parada JP, Johnson S, Gerding DN (2010) Effectiveness of alcohol-based hand rubs for removal of Clostridium difficile spores from hands. Infect Control Hosp Epidemiol 31(6):565–570. doi:10.1086/652772

    Article  PubMed  Google Scholar 

  31. Mela S, Whitworth DE (2014) The fist bump: a more hygienic alternative to the handshake. Am J Infect Control 42(8):916–917. doi:10.1016/j.ajic.2014.04.011

    Article  PubMed  Google Scholar 

  32. Meadow JF, Bateman AC, Herkert KM, O’Connor TK, Green JL (2013) Significant changes in the skin microbiome mediated by the sport of roller derby. Peer J 1:e53. doi:10.7717/peerj.53

    Article  PubMed Central  PubMed  Google Scholar 

  33. Lax S, Smith DP, Hampton-Marcell J, Owens SM, Handley KM, Scott NM, Gibbons SM, Larsen P, Shogan BD, Weiss S, Metcalf JL, Ursell LK, Vázquez-Baeza Y, Van Treuren W, Hasan NA, Gibson MK, Colwell R, Dantas G, Knight R, Gilbert JA (2014) Longitudinal analysis of microbial interaction between humans and the indoor environment. Science 345(6200):1048–1052. doi:10.1126/science.1254529

    Article  CAS  PubMed  Google Scholar 

  34. Martínez-Bastidas T, Castro-del Campo N, Mena KD, Castro-del Campo N, León-Félix J, Gerba CP, Chaidez C (2014) Detection of pathogenic micro-organisms on children’s hands and toys during play. J Appl Microbiol 116(6):1668–1675. doi:10.1111/jam.12473

    Article  PubMed  Google Scholar 

  35. Mygind O, Rønne T, Søe AL, Henrik Wachmann C, Ricks P (2003) Comparative intervention study among Danish daycare children: the effect on illness of time spent outdoors. Scand J Public Health 31(6):439–443. doi:10.1080/14034940310005349

    Article  PubMed  Google Scholar 

  36. Scott E, Bloomfield SF (1990) The survival and transfer of microbial contamination via cloths, hands and utensils. J Appl Bacteriol 68(3):271–278

    Article  CAS  PubMed  Google Scholar 

  37. Fritz SA, Epplin EK, Garbutt J, Storch GA (2009) Skin infection in children colonized with community-associated methicillin-resistant Staphylococcus aureus. J Infect 59(6):394–401. doi:10.1016/j.jinf.2009.09.001

    Article  PubMed Central  PubMed  Google Scholar 

  38. Gerhardts A, Hammer TR, Balluff C, Mucha H, Hoefer D (2012) A model of the transmission of micro-organisms in a public setting and its correlation to pathogen infection risks. J Appl Microbiol 112(3):614–621. doi:10.1111/j.1365-2672.2012.05234.x

    Article  CAS  PubMed  Google Scholar 

  39. Barker J, Jones MV (2005) The potential spread of infection caused by aerosol contamination of surfaces after flushing a domestic toilet. J Appl Microbiol 99(2):339–347. doi:10.1111/j.1365-2672.2005.02610.x

    Article  CAS  PubMed  Google Scholar 

  40. Hambraeus A (1973) Transfer of Staphylococcus aureus via nurses’ uniforms. J Hyg (Lond) 71(4):799–814

    Article  CAS  Google Scholar 

  41. Marples RR, Towers AG (1979) A laboratory model for the investigation of contact transfer of micro-organisms. J Hyg (Lond) 82(2):237–248

    Article  CAS  Google Scholar 

  42. Mackintosh CA, Hoffman PN (1984) An extended model for transfer of micro-organisms via the hands: differences between organisms and the effect of alcohol disinfection. J Hyg (Lond) 92(3):345–355

    Article  CAS  Google Scholar 

  43. Takashima M, Shirai F, Sageshima M, Ikeda N, Okamoto Y, Dohi Y (2004) Distinctive bacteria-binding property of cloth materials. Am J Infect Control 32(1):27–30. doi:10.1016/j.ajic.2003.05.003

    Article  PubMed  Google Scholar 

  44. Teufel L, Pipal A, Schuster KC, Staudinger T, Redl B (2010) Material-dependent growth of human skin bacteria on textiles investigated using challenge tests and DNA genotyping. J Appl Microbiol 108(2):450–461. doi:10.1111/j.1365-2672.2009.04434.x

    Article  CAS  PubMed  Google Scholar 

  45. Cotner S, Navrotski E, Sewera L, Snyder V, Richter E (2010) Diversity of culturable bacteria on natural vs artificial fabrics. Internet J Microbiol 8(2)

  46. Hsieh YL, Merry J (1986) The adherence of Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli on cotton, polyester and their blends. J Appl Bacteriol 60(6):535–544

    Article  CAS  PubMed  Google Scholar 

  47. Hsieh Y-L, Timm DA, Merry J (1987) Bacterial adherence on fabrics by a radioisotope labeling method. Text Res J 57(1):20–28

    Article  CAS  Google Scholar 

  48. Bajpai V, Dey A, Ghosh S, Bajpai S, Jha MK (2011) Quantification of bacterial adherence on different textile fabrics. Int Biodeterior Biodegrad 65(8):1169–1174

    Article  CAS  Google Scholar 

  49. St Sauver J, Khurana M, Kao A, Foxman B (1998) Hygienic practices and acute respiratory illness in family and group day care homes. Public Health Rep 113(6):544–551

    PubMed Central  CAS  PubMed  Google Scholar 

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Conflict of interest

The authors declare that they have no conflict of interest.

Compliance of ethics

This article does not contain any studies with human participants performed by any of the authors.

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Authors and Affiliations

  1. Hohenstein Institut für Textilinnovation gGmbH, Hygiene, Environment & Medicine, Schloss Hohenstein, 74357, Boennigheim, Germany

    A. Gerhardts & D. Höfer

  2. University of Applied Sciences Rhein-Waal, Marie-Curie-Str. 1, 47533, Kleve, Germany

    S. V. Henze & D. Bockmühl

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  1. A. Gerhardts
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  2. S. V. Henze
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  4. D. Höfer
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Correspondence to A. Gerhardts.

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Gerhardts, A., Henze, S.V., Bockmühl, D. et al. Fabric-skin models to assess infection transfer for impetigo contagiosa in a kindergarten scenario. Eur J Clin Microbiol Infect Dis 34, 1153–1160 (2015). https://doi.org/10.1007/s10096-015-2336-7

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  • DOI: https://doi.org/10.1007/s10096-015-2336-7

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