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Prevalence of carbapenem-hydrolyzing class D β-lactamase genes in Acinetobacter spp. isolates in China

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Abstract

In order to assess the prevalence of carbapenem-hydrolyzing class D β-lactamase genes in Acinetobacter spp. isolates in China, we conducted a polymerase chain reaction (PCR)-based surveillance of OXA-type β-lactamase gene clusters for a total of 2,880 Acinetobacter spp. isolates collected from 23 Chinese provinces. All isolates were tested for susceptibility to 12 antimicrobial agents and showed high rates of resistance to all these agents except minocycline. We also found that the vast majority of carbapenem-resistant Acinetobacter spp. were OXA-23-like-producing isolates, predominantly Acinetobacter baumannii isolates. Besides, bla OXA-58-like and bla OXA-24-like genes were detected in 32 and 11 isolates, respectively, involving many provinces throughout China. Furthermore, these two carbapenem-resistance determinants were located on transferable plasmids in most cases, indicating an emerging threat for both OXA-58-like- and OXA-24-like-producing Acinetobacter spp. isolates in China. Interestingly, a novel homologue of the bla OXA-143 gene was identified in a susceptible Acinetobacter pittii isolate. Overall, these observations suggest that the bla OXA-23-harboring A. baumannii isolates are the most frequent carbapenem-resistant Acinetobacter spp. in China, and the bla OXA-24-like and bla OXA-58-like genes have emerged as potential threats of hospital outbreaks of multidrug-resistant Acinetobacter spp.

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Acknowledgments

We would like to express our gratitude to Commissariat à l’Energie Atomique/Direction des Sciences du Vivant for kindly providing the Acinetobacter baylyi ADP1 strain for us.

We thank Michael Otto for his critical review of the manuscript.

Funding

This work was supported by the Ministry of Health of the People’s Republic of China (no. 201002021) and the National Natural Science Foundation of China (nos. NSFC81230039 and NSFC81301459).

Competing interests

None declared.

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Authors and Affiliations

  1. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China

    S. Ji, Y. Chen, Z. Ruan, Y. Fu, J. Ji, H. Wang & Y. Yu

  2. Department of Respiratory Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China

    Y. Fu

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  1. S. Ji
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  2. Y. Chen
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  3. Z. Ruan
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  4. Y. Fu
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  5. J. Ji
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  7. H. Wang
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  8. Y. Yu
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Corresponding author

Correspondence to Y. Yu.

Additional information

Shujuan Ji and Yan Chen contributed equally to this article.

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Ji, S., Chen, Y., Ruan, Z. et al. Prevalence of carbapenem-hydrolyzing class D β-lactamase genes in Acinetobacter spp. isolates in China. Eur J Clin Microbiol Infect Dis 33, 989–997 (2014). https://doi.org/10.1007/s10096-013-2037-z

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  • DOI: https://doi.org/10.1007/s10096-013-2037-z

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