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Assessment of the presence of extended-spectrum beta-lactamase-producing Escherichia coli in eggshells and ready-to-eat products

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Abstract

The principal objective of this study was to assess whether chicken eggshells may be contaminated by ESBL-producing E. coli (ESBLC). Additional analyses were carried out to determine if ESBLEC could be detected in other foodstuffs such as cooked poultry or fresh vegetables. Seventy-two eggshells from different supermarkets and stores as well as 32 salads, 30 samples of cooked poultry and six samples of chicken-based pet food samples were analysed. Characterization of ESBL was performed by PCR and sequencing. Antimicrobial resistance was determined by disk diffusion method. Phylogenetic group was assigned by multiplex-PCR. No ESBLEC was isolated from chicken eggshells, cooked chicken and pet food. One SHV-12-producing E. coli was isolated from a salad sample. This is the first study to analyse chicken eggs in an area where there is a high prevalence of ESBLEC in retail chicken meat.

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Acknowledgments

This study was supported by the Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III - FEDER, Spanish Network for Research into Infectious Diseases (REIPI RD06/0008), Fondo de Investigación Sanitaria (FIS), Instituto de Salud Carlos III (PI070190), Consejerías de Salud e Innovación, Junta de Andalucía (PI-0048/2008 and CTS-5259) and the Ministerio de Educación y Ciencia (AGL-2008-02129). We thank Beatriz Muñoz Hurtado for her assistance in European and Spanish legislation.

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Authors and Affiliations

  1. Infectious Diseases and Clinical Microbiology Unit, University Hospital Virgen Macarena, Avda Dr. Fedriani s/n., 41009, Seville, Spain

    P. Egea, L. López-Cerero, M. D. Navarro, J. Rodríguez-Baño & A. Pascual

  2. Department of Internal Medicine, School of Medicine, University of Seville, Seville, Spain

    J. Rodríguez-Baño

  3. Department of Microbiology, School of Medicine, University of Seville, Seville, Spain

    A. Pascual

Authors
  1. P. Egea
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  2. L. López-Cerero
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  3. M. D. Navarro
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  4. J. Rodríguez-Baño
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  5. A. Pascual
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Correspondence to P. Egea.

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Egea, P., López-Cerero, L., Navarro, M.D. et al. Assessment of the presence of extended-spectrum beta-lactamase-producing Escherichia coli in eggshells and ready-to-eat products. Eur J Clin Microbiol Infect Dis 30, 1045–1047 (2011). https://doi.org/10.1007/s10096-011-1168-3

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  • DOI: https://doi.org/10.1007/s10096-011-1168-3

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