Abstract
Whipple’s disease is a systemic chronic infection caused by Tropheryma whipplei. Asymptomatic people may carry T. whipplei in their digestive tract and this can be determined by PCR, making serological diagnosis useful to distinguish between carriers and patients. Putative antigenic proteins were selected by computational analysis of the T. whipplei genome, immunoproteomics studies and from literature. After expression, putative T. whipplei antigens were screened by microimmunofluorescence with sera of immunized rabbit. Selected targets were screened by microarray using sera from patients and carriers. Paradoxically, with 19 tested recombinant proteins and a glycosylated native protein of T. whipplei, a higher immune response was observed with asymptomatic carriers. In contrast, quantification of human IgA exhibited a higher reaction in patients than in carriers against 10 antigens. These results were used to design a diagnostic test with a cut-off value for each antigen. A blind test assay was performed and was able to diagnose 6/8 patients and 11/12 carriers. Among people with positive T. whipplei PCR of the stool, patients differ from carriers by having positive IgA detection and a negative IgG detection. If confirmed, this serological test will distinguish between carriers and patients in people with positive PCR of the stool.
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Acknowledgment
We thank Inodiag for technical help and for use of their technology.
We thank Isabelle Combe for helping in formatting the manuscript.
Conflicts of interest statement
Didier RAOULT is a cofounder of INODIAG, a start-up localized in LA CIOTAT France.
Financial support
This work was supported by a grant from the fifth Framework Program of the European Commission (QL G1-CT-2002–01049) and a grant for Programme Hospitalier de Recherche Clinique from the French Ministry of Health.
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Bonhomme, C.J., Renesto, P., Nandi, S. et al. Serological microarray for a paradoxical diagnostic of Whipple’s disease. Eur J Clin Microbiol Infect Dis 27, 959–968 (2008). https://doi.org/10.1007/s10096-008-0528-0
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DOI: https://doi.org/10.1007/s10096-008-0528-0