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Plasma levels of sirtuin-1 in patients with cerebrovascular stroke

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Abstract

Background

In Egypt, circulatory system diseases are responsible for one-third of the annual deaths. Stroke comes 3rd after heart diseases and liver diseases. Stroke includes two types: ischemic and hemorrhagic. The sirtuins (SIRTs) are a family of histone deacetylases that are nicotinamide adenine dinucleotide (NAD)+ dependent. They are activated under conditions of decreased cellular energy stores and are involved in the control of several physiological processes.

Objectives

To measure the plasma levels of SIRT1 in acute cerebrovascular stroke, to assess its role as a possible biomarker in predicting the risk of acute cerebrovascular stroke, to compare its levels between the two groups of stroke patients, and to evaluate the association between its levels and the severity of stroke. Also, to assess the correlations between the plasma SIRT1 levels and the variables that might play a role in the severity of acute cerebrovascular stroke.

Methods

This is a case-control study carried out on one hundred and eight participants. The participants were divided into two groups: group A (control group) included fifty-four individuals. Group B (acute cerebrovascular stroke group) included fifty-four stroke patients of two subgroups: B1: twenty-eight patients suffering from acute ischemic stroke and B2: twenty-six patients suffering from acute hemorrhagic stroke. Measurement of the plasma levels of SIRT1 was performed using the enzyme-linked immunosorbent assay (ELISA).

Results

Regarding SIRT1 levels, acute stroke groups were significantly lower than the control group with no significant difference between ischemic and hemorrhagic groups. There were positive correlations between SIRT1 levels and each of the hemoglobin levels and serum potassium levels. There were negative correlations between SIRT1 levels and each of triglycerides (TG) and stroke score.

Conclusion

Plasma levels of SIRT1 are lower in patients with acute cerebrovascular stroke than in control. Furthermore, SIRT1 may act as a possible biomarker for predicting the risk of acute cerebrovascular stroke.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Abbreviations

NAD+:

Nicotinamide adenine dinucleotide

SIRTs:

Silent mating-type information regulation 2

CVD:

Cardiovascular diseases

CBC:

Complete blood count

TC:

Total cholesterol

TG:

Triglycerides

LDL-C:

Low-density lipoprotein-cholesterol

HDL-C:

High-density lipoprotein-cholesterol

ALT:

Alanine transaminase

AST:

Aspartate transaminase

PT:

Prothrombin time

INR:

International normalized ratio

ELISA:

Enzyme-linked immunosorbent assay

BBB:

Blood-brain barrier

ICH:

Intracerebral hemorrhage

SAH:

Subarachnoid hemorrhage

CHD:

Coronary heart diseases

ROC:

Receiver operating curve

IGF-1:

Insulin-like growth factor-1

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Author information

Authors and Affiliations

  1. Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Imam M. Esmayel, Ehab A. Gohar & Mayada M. Mousa

  2. Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Samia Hussein

  3. Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Huda F. Ebian

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  1. Imam M. Esmayel
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  2. Samia Hussein
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  4. Huda F. Ebian
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Contributions

IME, MMM and SH designed and directed the project. SH, HFE, and EG performed the experiments and analyzed the data. MMM and SH wrote the manuscript. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Samia Hussein.

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The authors declare no competing interests.

Ethics approval and consent to participate

Written informed consent was obtained from each participant in the study. The study was approved by Institution Review Board, Faculty of Medicine, Zagazig University. Approval number: ZU-IRB#3931-13-8-2017

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Informed consent was obtained from all individual participants included in the study.

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Esmayel, I.M., Hussein, S., Gohar, E.A. et al. Plasma levels of sirtuin-1 in patients with cerebrovascular stroke. Neurol Sci 42, 3843–3850 (2021). https://doi.org/10.1007/s10072-021-05074-9

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  • DOI: https://doi.org/10.1007/s10072-021-05074-9

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