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Development and metabolic profiling of a postbiotic complex exhibiting antibacterial activity against skin microorganisms and anti-inflammatory effect on human keratinocytes

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Abstract

Beyond probiotics, the interest in the application of postbiotics to various fields has been growing. We aimed to develop a novel postbiotic complex (PC) with antibacterial and anti-inflammatory properties. Through antibacterial activity testing against Staphylococcus aureus or Cutibacterium acnes, a PC [a mixture of cell-free supernatants (postbiotics) from probiotic Lactobacillus helveticus (HY7801) and Lactococcus lactis (HY449)] was developed. Anti-inflammatory activity of the PC was investigated using HaCaT keratinocytes treated with S. aureus or C. acnes. PC significantly decreased IL-8 levels and increased hyaluronic acid levels in HaCaT cells cultured with S. aureus or C. acnes. GC–MS based metabolic profiling suggested 2-hydroxyisocaproic acid, hypoxanthine, succinic acid, ornithine, and γ-aminobutyric acid as potential contributing metabolites for the antibacterial and anti-inflammatory effects of PC. The PC developed in this study could be utilized in food, cosmetics, and pharmaceutical products as an alternative or complementary resources of probiotics.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (NRF-2022R1A5A6000760) and by the Chung-Ang University Graduate Research Scholarship of Republic of Korea in 2021.

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Authors and Affiliations

  1. College of Pharmacy, Chung-Ang University, Heukseok-ro 84, Dongjak-gu, Seoul, 06974, Republic of Korea

    Hyuk-Jin Chung, Hwanhui Lee, Myeongsun Kim, Ji Won Lee, Maham Saeed & Hyung-Kyoon Choi

  2. Hy Co., Ltd., 22 Giheungdanji-ro 24beon-gil, Giheung-gu, Yongin-si, Gyeonggi-do, 17086, Republic of Korea

    Hyuk-Jin Chung, Hayera Lee, Seung-Hee Jung, Jae-Jung Shim, Jung-Lyoul Lee & Keon Heo

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  1. Hyuk-Jin Chung
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  2. Hwanhui Lee
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Correspondence to Keon Heo or Hyung-Kyoon Choi.

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Chung, HJ., Lee, H., Kim, M. et al. Development and metabolic profiling of a postbiotic complex exhibiting antibacterial activity against skin microorganisms and anti-inflammatory effect on human keratinocytes. Food Sci Biotechnol 31, 1325–1334 (2022). https://doi.org/10.1007/s10068-022-01123-x

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