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Enzyme activity and partial characterization of proteases obtained from Bromelia karatas fruit and compared with Bromelia pinguin proteases

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Abstract

The enzymatic activity and partial characterization of proteases from Bromelia karatas fruits were evaluated and compared with Bromelia pinguin proteases. The specific activity increased twofold after partial purification in both proteases. Partially purified proteases from Bromelia karatas showed good specific activity at pH 6.0–8.0 and residual activity of 70–100% for 60 min at 37–60 °C, similar to Bromelia pinguin proteases. The K m value of proteases from Bromelia karatas was higher (253.32 µM) than that of Bromelia pinguin proteases (234.94 µM). The use of specific protease inhibitors indicated the presence of cysteine and serine proteases. Proteases with molecular weight of 66.2–97 and 21–31 kDa were detected. Bromelia karatas proteases registered 73% hydrolysis using a soy protein concentrate, similar to the enzyme activity of Bromelia pinguin proteases and commercial bromelain. These results demonstrate that Bromelia karatas proteases could be a potential alternative protease in the food industry.

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Acknowledgements

The authors acknowledge Tecnológico Nacional de Mexico (Grant No. 5613.15-P) for funding.

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Authors and Affiliations

  1. Laboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de Tepic, Av Tecnológico No. 2595, Lagos del Country, C.P. 63175, Tepic, Nayarit, Mexico

    Libier Meza-Espinoza, María de Lourdes García-Magaña, Sonia G. Sáyago-Ayerdi, Alejandra Chacón-López & Efigenia Montalvo-González

  2. Instituto Tecnológico de Tuxtepec, Av. Dr. Víctor Bravo Ahuja S/N, Col. 5 de Mayo, C.P. 68350, Tuxtepec, Oaxaca, Mexico

    María de los Ángeles Vivar-Vera

  3. Unidad Académica de Ciencias Químicas Biológicas y Farmacéuticas, Universidad Autónoma de Nayarit, Ciudad de la Cultura Amado Nervo S/N, Los Frenos, C.P. 63155, Tepic, Nayarit, Mexico

    Eduardo M. Becerrea-Verdín

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  1. Libier Meza-Espinoza
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  2. María de los Ángeles Vivar-Vera
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Correspondence to Efigenia Montalvo-González.

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Meza-Espinoza, L., de los Ángeles Vivar-Vera, M., de Lourdes García-Magaña, M. et al. Enzyme activity and partial characterization of proteases obtained from Bromelia karatas fruit and compared with Bromelia pinguin proteases. Food Sci Biotechnol 27, 509–517 (2018). https://doi.org/10.1007/s10068-017-0244-6

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  • DOI: https://doi.org/10.1007/s10068-017-0244-6

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