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Micro-CT evaluation of high pressure-decellularized cardiovascular tissues transplanted in rat subcutaneous accelerated-calcification model

  • Original Article
  • Tissue Engineering / Regenerative Medicine
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Abstract

We have succeeded in reducing the calcification of acellular aortas or valves in porcine allogeneic system by removing the DNA and phospholipids, but its further reduction is desirable. Here, the calcification of the acellular tissue was evaluated in rat subcutaneous transplantation model which is known as calcification model. Acellular samples prepared by high-hydrostatic pressure (HHP) protocols with different washing media were implanted and the calcification was monitored under micro-computed tomography for 1 and 3 months. The amount of the calcium deposition was quantitatively evaluated by atomic absorption spectroscopy. A cell culture medium showed very good cell removal ability but led to severe calcification at 1 month, and surprisingly the calcium deposition increased as the washing period increased. This calcification was suppressed by removing the DNA fraction with high DNase concentration. On the other hand, the calcification was greatly reduced when washed with saline even at low DNase concentration after 2 weeks washing. These results suggest that the ion species in the washing medium and the residual DNase cooperatively affect the tendency of in vivo calcification, which led us to the possibility of reduced calcification of acellular cardiac tissues.

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Acknowledgments

A part of this research was supported by the S-innovation Research Program for the “Development of the biofunctional materials for realization of innovative medicine”, Japan Science and Technology Agent (JST).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan

    Atsushi Mahara, Mitsuru Sago, Haruka Yamaguchi, Tomo Ehashi, Toshiya Fujisato & Tetsuji Yamaoka

  2. Department of Clinical Engineering, Suzuka University of Medical Science, Suzuka, Japan

    Mitsuru Sago, Haruka Yamaguchi & Toshiyuki Moritan

  3. Department of Cardiovascular Surgery, National Cerebral and Cardiovascular Center, Suita, Japan

    Kenji Minatoya & Hiroshi Tanaka

  4. Department of Transplantation, National Cerebral and Cardiovascular Center, Suita, Japan

    Takeshi Nakatani

  5. Department of Biomedical Engineering, Osaka Institute of Technology, Osaka, Japan

    Toshiya Fujisato

Authors
  1. Atsushi Mahara
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  2. Mitsuru Sago
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  3. Haruka Yamaguchi
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  4. Tomo Ehashi
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  7. Takeshi Nakatani
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  8. Toshiyuki Moritan
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  9. Toshiya Fujisato
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  10. Tetsuji Yamaoka
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Corresponding author

Correspondence to Tetsuji Yamaoka.

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Mahara, A., Sago, M., Yamaguchi, H. et al. Micro-CT evaluation of high pressure-decellularized cardiovascular tissues transplanted in rat subcutaneous accelerated-calcification model. J Artif Organs 18, 143–150 (2015). https://doi.org/10.1007/s10047-014-0808-7

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  • DOI: https://doi.org/10.1007/s10047-014-0808-7

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