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Local administration of TGF-β1 to reinforce the anterior abdominal wall in a rat model of incisional hernia

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Abstract

The purpose of this study was to investigate different forms of the local application of TGF-β1 for augmentation of the anterior abdominal wall in an appropriate model of an incisional hernia. Sixty male Sprague Dawley rats were divided into six groups. Artificial defects of the anterior abdominal wall were closed with one of the following methods: running Prolene suture, Vicryl mesh, prolene suture followed by an intramuscular injection of 1 μg TGF-β1, Vicryl mesh coated with 1 μg TGF-β1, and prolene suture coated with 1 μg TGF-β1. A control group did not receive any defect and treatment. Six weeks after operation, tensile strength, collagen content, gene expression of collagen I and III, blood vessels, and thickness of collagen fibres were evaluated. Tensile strength was strongest in the controls (14.2 (10.5–18 N)). There was no increase in tensile strength due to the administration of TGF-β1. On the contrary, bolus injection of the growth factor resulted in a significantly decreased strength of the wound tissue when compared to the groups 1, 4, 5, and 6 (9.1 (4.2–9.1 N)). These results correlated with the gene expression of collagen I and III. Local application of TGF-β1 did not augment the strength of the abdominal wall after 6 weeks.

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References

  1. Hunt TK, Knighton DR, Thakral KK, Goodson WH III, Andrews WS (1984) Studies on inflammation and wound healing: angiogenesis and collagen synthesis stimulated in vivo by resident and activated wound macrophages. Surgery 96:48–54

    PubMed  CAS  Google Scholar 

  2. Vogt PM, Drucke D, Muhlberger T, Homann HH, Steinau HU (2000) Clinical application of growth factors and cytokines in wound healing. Zentralbl Chir 125(Suppl 1):65–68

    PubMed  Google Scholar 

  3. Mustoe TA, Pierce GF, Thomason A, Gramates P, Sporn MB, Deuel TF (1987) Accelerated healing of incisional wounds in rats induced by transforming growth factor-beta. Science 237:1333–1336

    Article  PubMed  CAS  Google Scholar 

  4. Debus ES, Schmidt K, Ziegler UE, Thiede A (2000) Die Rolle der Wachstumsfaktoren in der Wundheilung. Zentralbl Chir 125:49–55

    PubMed  Google Scholar 

  5. Franz MG, Kuhn MA, Nguyen K, Wang X, Ko F, Wright TE, Robson MC (2001) Transforming growth factor beta(2) lowers the incidence of incisional hernias. J Surg Res 97:109–116

    Article  PubMed  CAS  Google Scholar 

  6. Wu L, Siddiqui A, Morris DE, Cox DA, Roth SI, Mustoe TA (1997) Transforming growth factor β3 (TGF β3) accelerates wound healing without alteration of scar prominence. Histologic and competitive reverse-transcription-polymerase chain reaction studies. Arch Surg 132:753–760

    PubMed  CAS  Google Scholar 

  7. Franz MG, Kuhn MA, Nguyen K, Wang X, Ko F, Wright TE, Robson MC (2001) Transforming growth factor β2 lowers the incidence of incisional hernias. J Surg Res 97:109–116

    Article  PubMed  CAS  Google Scholar 

  8. Polo M, Smith PD, Kim YJ, Wang X, Ko F, Robson MC (1999) Effect of TGF-beta2 on proliferative scar fibroblast cell kinetics. Ann Plast Surg 43:185–190

    PubMed  CAS  Google Scholar 

  9. Wang X, Smith P, Pu LL, Kim YJ, Ko F, Robson MC (1999) Exogenous transforming growth factor β2 modulates collagen I and collagen III synthesis in proliferative scar xenografts in nude rats. J Surg Res 87:194–200

    Article  PubMed  CAS  Google Scholar 

  10. Kuhn MA, Smith PD, Wachtel TL, Wright TE, Rogazewski A, Nguyen K, Robson MC, Franz MG (2001) Abdominal wall repair is delayed during hepatic regeneration. J Surg Res 95:54–60

    Article  PubMed  CAS  Google Scholar 

  11. Mustoe TA, Pierce GF, Thomason A, Gramates P, Sporn MB (1987) Accelerated healing of incisional wounds in rats induced by transforming growth factor-beta. Science 237:1333–1336

    Article  PubMed  CAS  Google Scholar 

  12. Struck H, Nagelschmidt M (1977) Hydroxyprolinfraktionen im Blut. J Clin Chem Clin Biochem 15:625–628

    PubMed  CAS  Google Scholar 

  13. Cengiz Y, Israelsson LA (1998) Incisional hernias in medline incisions: an eight-year follow up. Hernia 2:175–177

    Article  Google Scholar 

  14. Korenkov M, Sauerland S, Arndt M, Bograd L, Neugebauer EA, Troidl H (2002) Randomized clinical trial of suture repair, polypropylene mesh or autodermal hernioplasty for incisional hernia. Br J Surg 89:50–56

    Article  PubMed  CAS  Google Scholar 

  15. Roesel JF, Nanney LB (1995) Assessment of differential cytokine effects on angiogenesis using an in vivo model of cutaneous wound repair. J Surg Res 58:449–459

    Article  PubMed  CAS  Google Scholar 

  16. Iwasawa M (1993) Accelerated maturation in prefabricated flaps by transforming growth factor-beta: an experimental study in the rabbit. Ann Plast Surg 31:72–75

    Article  PubMed  CAS  Google Scholar 

  17. Raschke M, Wildemann B, Inden P, Bail H, Flyvbjerg A, Hoffmann J, Haas NP, Schmidmaier G (2002) Insulin-like growth factor-1 and transforming growth factor-beta1 accelerates osteotomy healing using polylactide-coated implants as a delivery system: a biomechanical and histological study in minipigs. Bone 30:144–151

    Article  PubMed  CAS  Google Scholar 

  18. Ueda H, Hong L, Yamamoto M, Shigeno K, Inoue M, Toba T, Yoshitani M, Nakamura T, Tabata Y, Shimizu Y (2002) Use of collagen sponge incorporating transforming growth factor-beta1 to promote bone repair in skull defects in rabbits. Biomaterials 23:1003–1010

    Article  PubMed  CAS  Google Scholar 

  19. Ksander GA, Ogawa Y, Chu GH, McMullin H, Rosenblatt JS, McPherson JM (1990) Exogenous transforming growth factor-beta 2 enhances connective tissue formation and wound strength in guinea pig dermal wounds healing by secondary intent. Ann Surg 211:288–294

    PubMed  CAS  Google Scholar 

  20. Smith PD, Kuhn MA, Franz MG, Wachtel TL, Wright TE, Robson MC (2000) Initiating the inflammatory phase of incisional healing prior to tissue injury. J Surg Res 92:11–17

    Article  PubMed  CAS  Google Scholar 

  21. Tyrone JW, Marcus JR, Bonomo SR, Mogford JE, Xia Y, Mustoe TA (2000) Transforming growth factor β3 promotes fascial wound healing in a new animal model. Arch Surg 135:1154–1159

    Article  PubMed  CAS  Google Scholar 

  22. Postlethwaite AE, Keski-Oja J, Moses HL, Kang AH (1987) Stimulation of the chemotactic migration of human fibroblasts by transforming growth factor beta. J Exp Med 165:251–256

    Article  PubMed  CAS  Google Scholar 

  23. Sporn MB, Roberts AB (1988) Peptide growth factors are multifunctional. Nature 332:217–219

    Article  PubMed  CAS  Google Scholar 

  24. Ignotz RA, Endo T, Massague J (1987) Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-beta. J Biol Chem 262:6443–6446

    PubMed  CAS  Google Scholar 

  25. Roberts AB, Sporn MB, Assoian RK, Smith JM, Roche NS, Wakefield LM, Heine UI, Liotta LA, Falanga V, Kehrl JH et al (1986) Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro. Proc Natl Acad Sci U S A 83:4167–4171

    Article  PubMed  CAS  Google Scholar 

  26. Fukuda H, Motohiro T, Nakai K, Yamamichi K, Nakane Y, Fujisawa J, Hioki K (2001) Negative effect of transforming growth factor-beta-1 on intestinal anastomotic tissue regeneration. Eur Surg Res 33:388–394

    Article  PubMed  CAS  Google Scholar 

  27. Hildebrand KA, Hiraoka H, Hart DA, Shrive NG, Frank CB (2002) Exogenous transforming growth factor beta 1 alone does not improve early healing of medial collateral ligament in rabbits. Can J Surg 45:330–336

    PubMed  Google Scholar 

  28. Huang JS, Wang YH, Ling TY, Chuang SS, Johnson FE, Huang SS (2002) Synthetic TGF-beta antagonist accelerates wound healing and reduces scarring. Faseb J 16:1269–1270

    PubMed  CAS  Google Scholar 

  29. Cox DA, Kunz S, Cerletti N, McMaster GK, Burk RR (1992) Wound healing in aged animals–effects of locally applied transforming growth factor beta 2 in different model systems. Exs 61:287–295

    PubMed  CAS  Google Scholar 

  30. Tateshita T, Ono I, Kaneko F (2001) Effects of collagen matrix containing transforming growth factor (TGF)-beta(1) on wound contraction. J Dermatol Sci 27:104–113

    Article  PubMed  CAS  Google Scholar 

  31. Beck LS, DeGuzman L, Lee WP, Xu Y, Siegel MW, Amento EP (1993) One systemic administration of transforming growth factor-beta 1 reverses age- or glucocorticoid-impaired wound healing. J Clin Invest 92:2841–2849

    Article  PubMed  CAS  Google Scholar 

  32. Slavin J, Nash JR, Kingsnorth AN (1992) Effect of transforming growth factor beta and basic fibroblast growth factor on steroid-impaired healing intestinal wounds. Br J Surg 79:69–72

    Article  PubMed  CAS  Google Scholar 

  33. Song QH, Klepeis VE, Nugent MA, Trinkaus-Randall V (2002) TGF-beta1 regulates TGF-beta1 and FGF-2 mRNA expression during fibroblast wound healing. Mol Pathol 55:164–176

    Article  PubMed  CAS  Google Scholar 

  34. Huhn EA, Jannowitz C, Boos H, Papadopulos MA, Zeilhofer HF, Henke J, Mueller D, Kovacz L, Biemer E, Papadopulos NA (2004) Fetale Wundheilung. Aktueller Stand und neue Perspektiven. Chirurg 75:498–507

    Article  PubMed  CAS  Google Scholar 

  35. Cohen IK (1992) The skin. In: Lindblad WJ (ed) Wound healing. W.B. Saunders, Philadelphia, pp 344–355

    Google Scholar 

  36. Hoer JJ, Junge K, Schachtrupp A, Klinge U, Schumpelick V (2002) Influence of laparotomy closure technique on collagen synthesis in the incisional region. Hernia 6:93–98

    Article  PubMed  CAS  Google Scholar 

  37. Junge K, Klinge U, Rosch R, Mertens PR, Kirch J, Klosterhalfen B, Lynen P, Schumpelick V (2004) Decreased collagen type I/III ratio in patients with recurring hernia after implantation of alloplastic prostheses. Langenbecks Arch Surg 389:17–22

    Article  PubMed  Google Scholar 

  38. Wahl SM, Hunt DA, Wong HL, Dougherty S, McCartney-Francis N, Wahl LM, Ellingsworth L, Schmidt JA, Hall G, Roberts AB et al (1988) Transforming growth factor-beta is a potent immunosuppressive agent that inhibits IL-1-dependent lymphocyte proliferation. J Immunol 140:3026–3032

    PubMed  CAS  Google Scholar 

  39. Wahl SM, Hunt DA, Wakefield LM, McCartney-Francis N, Wahl LM, Roberts AB, Sporn MB (1987) Transforming growth factor type beta induces monocyte chemotaxis and growth factor production. Proc Natl Acad Sci U S A 84:5788–5792

    Article  PubMed  CAS  Google Scholar 

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Korenkov, M., Yuecel, N., Koebke, J. et al. Local administration of TGF-β1 to reinforce the anterior abdominal wall in a rat model of incisional hernia. Hernia 9, 252–258 (2005). https://doi.org/10.1007/s10029-005-0341-y

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  • DOI: https://doi.org/10.1007/s10029-005-0341-y

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