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Diversity of Crenarchaeota in terrestrial hot springs in Tengchong, China

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Abstract

Diversity of Crenarchaeota was investigated in eight terrestrial hot springs (pH 2.8–7.7; temperature 44–96°C) located in Tengchong, China, using 16S rRNA gene phylogenetic analysis. A total of 826 crenarchaeotal clones were sequenced and a total of 47 operational taxonomic units (OTUs) were identified. Most (93%) of the identified OTUs were closely related (89–99%) to those retrieved from hot springs and other thermal environments. Our data showed that temperature may predominate over pH in affecting crenarchaeotal diversity in Tengchong hot springs. Crenarchaeotal diversity in moderate-temperature (59–77°C) hot springs was the highest, indicating that the moderately hot-temperature springs may provide optimal conditions for speciation of Crenarchaeota.

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Acknowledgments

This research was supported by the National Basic Research Program of China (No. 2010CB833800), Key Project of International Cooperation (2007DFB31620), Yunnan Provincial Natural Science Foundation (Nos. 2009AC017, 2009DA002) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry. CLZ was supported by the US National Science Foundation grant # MCB-0348180.

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Authors and Affiliations

  1. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, China

    Zhao-Qi Song, Jing-Quan Chen, En-Min Zhou, Shu-Kun Tang, Xiao-Yang Zhi & Wen-Jun Li

  2. Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650011, China

    Zhao-Qi Song, Jing-Quan Chen, En-Min Zhou, Shu-Kun Tang, Xiao-Yang Zhi & Wen-Jun Li

  3. Geomicrobiology Laboratory, State Key Laboratory of Geological Processes and Mineral Resources, China University of Geosciences, Beijing, 100083, China

    Hong-Chen Jiang

  4. Chinese Academy of Sciences, Institute of Microbiology, Beijing, 100101, China

    Li-Xin Zhang & Wen-Jun Li

  5. Department of Marine Sciences, University of Georgia, Athens, GA, 30602, USA

    Chuan-Lun L. Zhang

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  1. Zhao-Qi Song
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  9. Wen-Jun Li
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Correspondence to Wen-Jun Li.

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Communicated by A. Oren.

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Song, ZQ., Chen, JQ., Jiang, HC. et al. Diversity of Crenarchaeota in terrestrial hot springs in Tengchong, China. Extremophiles 14, 287–296 (2010). https://doi.org/10.1007/s00792-010-0307-6

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