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Characterization of the cytoplasmic chaperonin containing TCP-1 from the Antarctic fish Notothenia coriiceps

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Abstract

The cytoplasmic chaperonin containing TCP-1 (CCT) plays a critically important role in the folding and biogenesis of many cytoskeletal proteins, including tubulin and actin. For marine ectotherms, the chronically cold Southern Ocean (−2 to +2°C) poses energetic challenges to protein folding, both at the level of substrate proteins and with respect to the chaperonin/chaperone folding system. Here we report the partial functional and structural characterization of CCT from an Antarctic notothenioid fish, Notothenia coriiceps. We find that the mechanism of folding by the Antarctic fish CCT differed from that of mammalian CCT: (1) the former complex was able to bind denatured β-tubulin but (2) when reconstituted with rabbit Cofactor A, failed to release the protein to yield the tubulin/cofactor intermediate. Moreover, the amino acid sequences of the N. coriiceps CCT β and θ chains contained residue substitutions in the equatorial, apical, and intermediate domains that would be expected to increase the flexibility of the subunits, thus facilitating function of the chaperonin in an energy poor environment. Our work contributes to the growing realization that protein function in cold-adapted organisms reflects a delicate balance between the necessity of structural flexibility for catalytic activity and the concomitant hazard of cold-induced denaturation.

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Abbreviations

CCT:

Cytoplasmic chaperonin containing tcp-1

TCP1:

Tcomplex protein 1

CofA:

Cofactor A

MES:

2-Morpholinoethanesulfonic acid, monohydrate

EGTA:

Ethylene glycol bis(2-aminoethyl ether)-N,N,N′N′-tetraacetic acid

DTT:

Dithiothreitol

SDS:

Sodium dodecyl sulfate

PAGE:

Polyacrylamide gel electrophoresis

EST:

Expressed sequence tags

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Acknowledgements

We acknowledge with gratitude the excellent logistic support provided to our Antarctic field research program, performed at Palmer Station and on the seas of the Palmer Archipelago, by the staff of the Office of Polar Programs of the National Science Foundation, by the personnel of Antarctic Support Associates and Raytheon Polar Services Company, and by the captain and crew of the R/V Laurence M. Gould. We thank Patricia Singer (University of Maine DNA Sequencing Facility) for her excellent performance of the automated DNA sequencing. This work was supported by National Science Foundation grants OPP-9815381, OPP-0089451, OPP-0336932, and the Association pour la Recherche sur le Cancer.

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Authors and Affiliations

  1. Dipartimento di Biologia, Molecolare, Cellulare, ed Animale, Università di Camerino, 62032, Camerino, Italy

    Sandra Pucciarelli

  2. Department of Biology, Northeastern University, Boston, MA, 02115, USA

    Sandra K. Parker & H. William Detrich III

  3. Laboratoire d’Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, 91198, Gif-sur-Yvette, France

    Ronald Melki

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  1. Sandra Pucciarelli
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  2. Sandra K. Parker
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  3. H. William Detrich III
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  4. Ronald Melki
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Correspondence to Sandra Pucciarelli.

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Communicated by K. Horikoshi

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Pucciarelli, S., Parker, S.K., Detrich, H.W. et al. Characterization of the cytoplasmic chaperonin containing TCP-1 from the Antarctic fish Notothenia coriiceps . Extremophiles 10, 537–549 (2006). https://doi.org/10.1007/s00792-006-0528-x

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