Abstract
Objectives
This paper aims to assess the whitening effectiveness and toxicity of tooth-bleaching protocols applied to enamel/dentin disks simulating mandibular incisors (ICs) and premolars (PMs).
Materials and methods
A 10% hydrogen peroxide (H2O2) gel was applied for 3 × 15, 1 × 15, or 1 × 5 min to enamel/dentin disks simulating mandibular ICs and PMs, and the trans-enamel and trans-dentinal diffusion products were applied to human dental pulp cells (1 h). Professional therapy (35% H2O2—3 × 15 min) was used as positive control, and non-bleached samples were used as negative control. Cell viability and morphology, oxidative stress generation, and odontoblastic marker expression were assessed. The H2O2 diffusion and enamel color change (ΔE) were also analyzed.
Results
The 10% H2O2 gel induced significant cell viability reduction only when applied 3 × 15 min, with the intensity of oxidative stress and down-regulation of odontoblastic markers being higher in the IC group. The other experimental bleaching protocols caused slight alterations regarding the cell parameters evaluated, with intensity being related to enamel/dentin thickness. These effects were also correlated with higher H2O2 diffusion in the IC group. ΔE values similar as positive control were found for the 10% 3 × 15 and 1 × 15 protocols on IC group, after 4 and 6 sessions.
Conclusion
Application of a 10% H2O2 bleaching gel for 15 or 45 min to thin dental substrate significantly minimizes cell toxicity in comparison with highly concentrated gels associated with similar esthetic outcomes by increasing the number of bleaching sessions.
Clinical relevance
Bleaching gels with 10% H2O2 applied in small teeth for short periods may be an interesting alternative to obtain whitening effectiveness without causing toxicity to pulp cells, which may be able to reduce the tooth hypersensitivity claimed by patients.
Similar content being viewed by others
References
de Almeida LC, Costa CA, Riehl H, dos Santos PH, Sundfeld RH, Briso AL (2012) Occurrence of sensitivity during at-home and in-office tooth bleaching therapies with or without use of light sources. Acta Odontol Latinoam 25:3–8
Bonafé E, Bacovis CL, Iensen S, Loguercio AD, Reis A, Kossatz S (2013) Tooth sensitivity and efficacy of in-office bleaching in restored teeth. J Dent 41:363–369
Rodrigues LM, Vansan LP, Pécora JD, Marchesan MA (2009) Permeability of different groups of maxillary teeth after 38% hydrogen peroxide internal bleaching. Braz Dent J 20:303–306
Roderjan DA, Stanislawczuk R, Hebling J, de Souza Costa CA, Reis A, Loguercio AD (2015) Response of human pulps to different in-office bleaching techniques: preliminary findings. Braz Dent J 26:242–248
Roderjan DA, Stanislawczuk R, Hebling J, de Souza Costa CA, Soares DG, Reis A, Loguercio AD (2014) Histopathological features of dental pulp tissue from bleached mandibular incisors. J Mater Sci Eng B 4:178–185
Kina JF, Huck C, Riehl H, Martinez TC, Sacono NT, Ribeiro AP, Costa CA (2010) Response of human pulps after professionally applied vital tooth bleaching. Int Endod J 43:572–580
De Souza Costa CA, Riehl H, Kina JF, Sacono NT, Hebling J (2010) Human pulp responses to in-office tooth bleaching. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:e59–e64
Soares DG, Basso FG, Hebling J, de Souza Costa CA (2014) Concentrations of and application protocols for hydrogen peroxide bleaching gels: effects on pulp cell viability and whitening efficacy. J Dent 42:185–198
Soares DG, Basso FG, Hebling J, de Souza Costa CA (2015) Immediate and late analysis of dental pulp stem cells viability after indirect exposition to alternative in-office bleaching strategies. Clin Oral Investig 19:1013–1020
Hanks CT, Craig RG, Diehl ML, Pashley DH (1998) Cytotoxicity of dental composites and other materials in a new in vitro device. J Oral Pathol 17:396–403
Gronthos S, Arthur A, Bartold PM, Shi S (2011) A method to isolate and culture expand human dental pulp stem cells. Methods Mol Biol 698:107–121
Soares DG, Basso FG, Scheffel DS, Hebling J, de Souza Costa CA (2015) Responses of human dental pulp cells after application of a low-concentration bleaching gel to enamel. Arch Oral Biol 60:1428–1436
Soares DG, Ribeiro AP, da Silveira VF, Hebling J, de Souza Costa CA (2013) Efficacy and cytotoxicity of a bleaching gel after short application times on dental enamel. Clin Oral Investig 17:1901–1909
Soares DG, Basso FG, Pontes EC, Garcia Lda F, Hebling J, de Souza Costa CA (2014) Effective tooth-bleaching protocols capable of reducing H(2)O(2) diffusion through enamel and dentine. J Dent 42:351–358
Lee DH, Lim BS, Lee YK, Yang HC (2006) Effects of hydrogen peroxide (H2O2) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines. Cell Biol Toxicol 22:39–46
Min KS, Lee HJ, Kim SH, Lee SK, Kim HR, Pae HO (2008) Hydrogen peroxide induces Heme Oxygenase-1 and dentin sialophosphoprotein mRNA in human pulp cells. J Endod 34:983–989
Matsui S, Takahashi C, Tsujimoto Y, Matsushima K (2009) Stimulatory effects of low-concentration reactive oxygen species on calcification ability of human dental pulp cells. J Endod 35:67–72
Lee YH, Kang YM, Heo MJ, Kim GE, Bhattarai G, Lee NH, Yu MK, Yi HK (2013) The survival role of peroxisome proliferator-activated receptor gamma induces odontoblast differentiation against oxidative stress in human dental pulp cells. J Endod 39:236–241
Sulieman M, Addy M, MacDonald E, Rees JS (2004) The effect of hydrogen peroxide concentration on the outcome of tooth whitening: an in vitro study. J Dent 32:295–299
Camargo SE, Valera MC, Camargo CH, Gasparoto Mancini MN, Menezes MM (2007) Penetration of 38% hydrogen peroxide into the pulp chamber in bovine and human teeth submitted to office bleach technique. J Endod 33:1074–1077
Chen JH, Xu JW, Shing CX (1993) Decomposition rate of hydrogen peroxide bleaching agents under various chemical and physical conditions. J Prosthet Dent 69:46–48
Palo RM, Bonetti-Filho I, Valera MC, Camargo CH, Camargo S, Moura-Netto C, Pameijer C (2012) Quantification of peroxide ion passage in dentin, enamel, and cementum after internal bleaching with hydrogen peroxide. Oper Dent 37:660–664
Duque CC, Soares DG, Basso FG, Hebling J, de Souza Costa CA (2014) Bleaching effectiveness, hydrogen peroxide diffusion, and cytotoxicity of a chemically activated bleaching gel. Clin Oral Investig 18:1631–1637
Acknowledgments
The authors acknowledge the São Paulo Research Foundation–FAPESP (grant #2014/07229-6), the National Council for Scientific and Technological Development–CNPq (grants #442336/2014-4 and 303599/2014-6), and the Brazilian Division of the IADR award (1st place, PRONAC/2014) for the financial support.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
This study was approved by the Research Ethics Committee of the Araraquara Dental School/UNESP, São Paulo, Brazil (Proc. No 3467314.0.0000.5416). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent
Informed consent was obtained from all individual participants included in the study.
Rights and permissions
About this article
Cite this article
de Oliveira Duque, C., Soares, D., Basso, F. et al. Influence of enamel/dentin thickness on the toxic and esthetic effects of experimental in-office bleaching protocols. Clin Oral Invest 21, 2509–2520 (2017). https://doi.org/10.1007/s00784-017-2049-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00784-017-2049-7