Skip to main content

Advertisement

SpringerLink
Log in

Antibacterial properties and human gingival fibroblast cell compatibility of TiO2/Ag compound coatings and ZnO films on titanium-based material

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Titanium (Ti)-based materials are widely used in biomedical implant components and are applied successfully in various types of bone-anchored reconstructions. However, in dental implants the Ti materials contact not only bone but also gingival tissues, and are partially exposed to the oral cavity that includes bacteria. This study used titania and silver (TiO2/Ag) compound coatings and zinc oxide (ZnO) films to enhance the antibacterial activity of the Ti-based implant. The hydrophobicity of each sample was examined by measuring the contact angle. Streptococcus mutans and human gingival fibroblast (HGF) was cultured on the coated samples, and the antibacterial effects and cell compatibility were determined using a Syto9 fluorescence staining and MTT methods. For the TiO2/Ag samples, depositing Ag on the plate at a higher power (which increased the proportion of Ag) increased the contact angle and the hydrophobicity. The bacterial count was lowest for the 50 W TiO2/Ag sample, which contained 5.9% Ag. The contact angles of the ZnO samples did not show the same tendency. The antibacterial effect was higher on ZnO-coated samples since bacterial count was threefold lower on ZnO samples as compared to control samples (Ti plate). From the MTT assay test, the mean optical density values for TiO2/Ag-coated samples after 72 h of HGF adhesion were similar to the value obtained from the uncoated Ti. However, biocompatibility was lower on ZnO films than in control samples. Conclusively, the antibacterial activity was higher but the cell compatibility was lower on ZnO films than on TiO2/Ag coatings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Zhou W, Zhong X, Wu X, Yuan L, Zhao Z, Wang H, Xia Y, Feng Y, He J, Chen W (2006) The effect of surface roughness and wettability of nanostructured TiO2 film on TCA-8113 epithelial-like cells. Surf Coat Technol 200:6155–6160

    Article  Google Scholar 

  2. Chiang CY, Chiou SH, Yang WE, Hsu ML, Yung MC, Tsai ML, Chen LK, Huang HH (2009) Formation of TiO(2) nano-network on titanium surface increases the human cell growth. Dent Mater 25:1022–1029

    Article  PubMed  Google Scholar 

  3. Schearz F, Sculean A, Romanos G, Herten M, Horn N, Scherbaum W, Becker J (2005) Influence of different treatment approaches on the removal of early plaque biofilms and the viability of SAOS2 osteoblasts grown on titanium implants. Clin Oral Investig 9:111–117

    Article  Google Scholar 

  4. Tsang CSP, Ng H, McMillan AS (2007) Antifungal susceptibility of Candida albicans biofilms on titanium discs with different surface roughness. Clin Oral Investig 11:361–368

    Article  PubMed  Google Scholar 

  5. Leonhardt A, Dahlen G (1995) Effect of titanium on selected oral bacterial species in vitro. Eur J Oral Sci 103:382–387

    Article  PubMed  Google Scholar 

  6. Colon G, Ward BC, Webster TJ (2006) Increased osteoblast and decreased Staphylococcus epidermidis functions on nanophase ZnO and TiO2. J Biomed Mater Res A 78:595–604

    PubMed  Google Scholar 

  7. Heydenrijk K, Meijer HJ, van der Reijden WA, Raghoebar GM, Vissink A, Stegenga B (2002) Microbiota around root-form endosseous implants: a review of the literature. Int J Oral Maxillofac Implants 17:829–838

    PubMed  Google Scholar 

  8. Cortese-Krott MM, Münchow M, Pirev E, Hessner F, Bozkurt A, Uciechowski P, Pallua N, Kröncke KD, Suschek CV (2009) Silver ions induce oxidative stress and intracellular zinc release in human skin fibroblasts. Free Radic Biol Med 47:1570–1577

    Article  PubMed  Google Scholar 

  9. Shirkhanzadeh M, Azadegan M, Liu GQ (1995) Bioactive delivery systems for the slow release of antibiotics: incorporation of Ag+ ions into micro-porous hydroxyapatite coatings. Mater Lett 24:7–12

    Article  Google Scholar 

  10. Necula BS, Fratila-Apachitei LE, Zaat SA, Apachitei I, Duszczyk J (2009) In vitro antibacterial activity of porous TiO2-Ag composite layers against methicillin-resistant Staphylococcus aureus. Acta Biomater 5:3573–3580

    Article  PubMed  Google Scholar 

  11. Ewald A, Glückermann SK, Thull R, Gbureck U (2006) Antimicrobial titanium/silver PVD coatings on titanium. Biomed Eng Online 5:22

    Article  PubMed  Google Scholar 

  12. Akhavan O, Ghaderi E (2009) Enhancement of antibacterial properties of Ag nanorods by electric field. Surf Coat Technol 203:3123–3128

    Article  Google Scholar 

  13. Geurtsen W, Leyhausen G (1997) Biological aspects of root canal filling materials—histocompatibility, cytotoxicity, and mutagenicity. Clin Oral Investig 1:5–11

    Article  PubMed  Google Scholar 

  14. Osamu Y, Miyako K, Jun S, Zenbe-e N (2004) The antibacterial effects of zinc ion migration from zinc-based glass polyalkenoate cements. J Mater Sci Mater Med 15:847–851

    Article  Google Scholar 

  15. Tam KH, Djurišić AB, Chan CMN, Xi YY, Tse CW, Leung YH, Chan WK, Leung FCC, Au DWT (2008) Antibacterial activity of ZnO nanorods prepared by a hydrothermal method. Thin Solid Films 516:6167–6174

    Article  Google Scholar 

  16. Richards RM, Odelola HA, Anderson B (1984) Effect of silver on whole cells and spheroplasts of a silver resistant Pseudomonas aeruginosa. Microbios 39:151–157

    PubMed  Google Scholar 

  17. Lok CN, Ho CM, Chen R, He QY, Yu WY, Sun H, Tam PKH, Chiu JF, Che CM (2006) Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteome Res 5:916–924

    Article  PubMed  Google Scholar 

  18. Park HJ, Kim JY, Kim J, Lee JH, Hahn JS, Gu MB, Yoon J (2009) Silver-ion-mediated reactive oxygen species generation affecting bactericidal activity. Water Res 43:1027–1032

    Article  PubMed  Google Scholar 

  19. Brown T, Smith D (1976) The effects of silver nitrate on the growth and ultrastructure of the yeast Cryptococcus albidus. Microbios Lett 3:155–162

    Google Scholar 

  20. Satou J, Fukunaga A, Satou N, Shintani H, Okuda K (1998) Streptococcal adherence on various restorative materials. J Dent Res 67:588–591

    Google Scholar 

  21. Xu B, Cai Z, Wang W, Ge F (2010) Preparation of superhydrophobic cotton fabrics based on SiO2 nanoparticles and ZnO nanorod arrays with subsequent hydrophobic modification. Surf Coat Technol 204:1556–1561

    Article  Google Scholar 

  22. Zhu X, Chen J, Scheideler L, Reich R, Geis-Gerstorfer J (2004) Effects of topography and composition of titanium surface oxides on osteoblast responses. Biomaterials 25:4087–4103

    Article  PubMed  Google Scholar 

  23. Lee J, Kang BS, Hicks B, Chancellor TF Jr, Chu BH, Wang HT, Keselowsky BG, Ren F, Lele TP (2008) The control of cell adhesion and viability by zinc oxide nanorods. Biomaterials 29:3743–3749

    Article  PubMed  Google Scholar 

  24. Kikuchi A, Okano T (2005) Nanostructured designs of biomedical materials: applications of cell sheet engineering to functional regenerative tissues and organs. J Control Release 101:69–84

    Article  PubMed  Google Scholar 

Download references

Conflict of interest

This research was supported by the Government Organization—National Science Council (NSC 98-2815-C-039-049-B) and (NSC 98-2320-B-039-005-MY3), Taiwan. All of the authors have no financial relationship to any private companies and organizations.

Author information

Authors and Affiliations

  1. Department of Materials Science and Engineering, Ming Dao University, 369 Wen-Hua Rd., Peetow, ChangHua, 52345, Taiwan

    Yin-Yu Chang

  2. School of Medicine, China Medical University and Hospital, 91 Hsueh-Shih Road, Taichung, 40402, Taiwan

    Chih-Ho Lai & Chih-Hsin Tang

  3. School of Dentistry, College of Medicine, China Medical University and Hospital, 91 Hsueh-Shih Road, Taichung, 40402, Taiwan

    Jui-Ting Hsu, Wan-Chuen Liao & Heng-Li Huang

Authors
  1. Yin-Yu Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chih-Ho Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jui-Ting Hsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chih-Hsin Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wan-Chuen Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Heng-Li Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Heng-Li Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, YY., Lai, CH., Hsu, JT. et al. Antibacterial properties and human gingival fibroblast cell compatibility of TiO2/Ag compound coatings and ZnO films on titanium-based material. Clin Oral Invest 16, 95–100 (2012). https://doi.org/10.1007/s00784-010-0504-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-010-0504-9

Keywords

Search

Navigation