Abstract
The aim of the present study was to evaluate comparatively the pulpal tissue reactions of four adhesive systems placed in experimental cavities of healthy dog’s teeth. Class V cavities with a mean value of remaining dentin thickness (RDT) ranging between 0.55 ± 0.30–0.68 ± 0.38 mm were prepared. The cavities were treated with the following adhesive systems: Etch and Prime 3.0 (EP), Single Bond (SB), Clearfil SE Bond (CSE), and Prompt L-Pop (PLP). The pulpal tissue responses to dentin adhesives were assessed histopathologically at postoperative periods of 7, 21, and 65 days, and the results were subjected to statistical analysis. A significantly greater adverse inflammatory response was observed with the materials EP and PLP, while a significantly lesser degree of disorganization in the odontoblastic zone was found with the materials SB and CSE, in the postoperative period of 65 days. In addition, a thicker predentin zone was observed where SB material was applied. Application of the selected adhesive systems to non-exposed cavities, with an RDT, which ranged between the above-mentioned rates, was correlated with slight to moderate inflammation and odontoblast reduction depending on the materials used as well as upon the RDT.
Similar content being viewed by others
References
About I, Murray PE, Franquin J-C, Remusat M, Smith AJ (2001) Pulpal inflammation responses following non-carious class V restorations. Oper Dent 26:336–342
Bouillaguet S, Wataha JC, Hanks CT, Ciucchi B, Holz J (1996) In vitro cytotoxicity and dentin permeability of HEMA. J Endod 22:244–248
Chan K, Tay F, King N, Imazato S, Pashley D (2003) Bonding of mild self-etching primers/adhesives to dentin with thick smear layers. Am J Dent 16:340–346
Costa CAS,Vaerten MA, Edwards CA, Hanks CT (1999) Cytotoxicity of current dental adhesive systems on immortalized odontoblast cell line MDPC-23. Dent Mater 15:434–441
Costa CAS, Hebling J, Hanks CT (2000) Current status of pulp capping with dentin adhesive systems: a review. Dent Mater 16:188–199
Costa CAS, Nascimento ABL, Teixeira HM (2002) Response of human pulps following acid conditioning and application of a bond agent in deep cavities. Dent Mater 18:543–551
Frankenberger R, Krämer N, Petschelt A (2000) Technique sensitivity of dentin bonding: effect of application mistakes on bond strength and marginal adaptation. Oper Dent 25:324–330
Frankenberger R, Perdigăo J, Rosa B, Lopes M (2001) “No bottle-bottle” vs “multi-bottle” dentin adhesives: a microtensile bond strength and morphological study. Dent Mater 17:373–380
Gwinnett AJ, Tay F (1998) Early and intermediate time response of the dental pulp to an acid etch technique in vivo. Am J Dent 11:S35–S44
Hanks CT, Strawn RR, Wataha JC, Craig RG (1991) Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res 70:1450–1455
Hashimoto M, Ohno H, Kaga M,Endo K, Sano H, Oguchi H (2000) In vivo degradation of resin–dentin bonds in humans over 1–3 years. J Dent Res 79:1385–1391
Hebling J, Giro EMA, Costa GAS (1999) Human pulp response after an adhesive system application in deep cavities. J Dent 27:557–564
Inoue S, Van Meerbeek B, Vargas M, Yoshida Y, Lambrechts P, Vanherle G (1999) Adhesion mechanism of self-etching adhesives. Am J Dent 10:131–175
Inoue S, van Meerbeek B, Vargas M, Yoshida Y, Lambrechts P, Vanherle G (2000) Adhesion mechanism of self-etching adhesives. In: Tagami J, Toledano M, Prati C (eds) Advanced adhesive dentistry. 3rd International Kuraray Symposium, 1999, Cranada, Spain. Crafiche Erredue, Crimido (Como), Italy, pp 131–148
Inoue S, Vargas MA, Abe Y, Yoshida Y, Lambrechts P, Vanherle G, Sano H, Van Meerbeek B (2001) Micro-tensile bond strength of eleven contemporary modern adhesives to dentine. J Adhes Dent 3:237–245
Lesot H, Begue-Kirn C, Kubler JD, Meyer JM, Smith AJ, Cassidy N, Ruch JV (1993) Experimental induction of odontoblast differentiation and stimulation during reparative processes. Cells Mater 3:201–217
Murray PE, Lumley PJ, Ross HF, Smith AJ (2000) Tooth Slice organ culture for cytotoxicity assessment of dental materials. Biomaterials 21:1711–1721
Murray PE, About I, Lumley PJ, Franquin J-C, Remusat M, Smith AJ (2002) Cavity remaining dentin thickness and pulpal activity. Am J Dent 15:41–46
Murray PE, Smith AJ, Windsor LJ, Mjör IA (2003) Remaining dentine thickness and human pulp responses. Int Endod J 36:33–43
Pashley EL, Agee KA, Pashley DH, Tay FR (2002) Effects of one versus two applications of an unfilled, all-in-one adhesive on dentine bonding. J Dent 30:83–90
Sano H, Yoshikawa T, Pereira PNR, Kanemura N, Morigami M, Tagami J, Pashley DH (1999) Long-term durability of dentin bonds made with a self-etching primer. J Dent Res 78:906–911
Stanley HR (1968) Design for a human pulp study. Part 1. Oral Surg Oral Med Oral Pathol 25:633–647
Tay FR, Gwinnet JA, Wei SHY (1998) Relation between water content in acetone/alcohol-based primer and interfacial ultrastructure. J Dent 26:147–156
Tay FR, Sano H, Carvalho R, Pashley E, Pashley DH (2000a) An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact dentin. J Adhes Dent 2:83–98
Tay FR, Carvalho R, Sano H, Pashley DH (2000b) Effect of smear layers on the bonding of a self -etching primer in dentin. J Adhes Dent 2:99–116
Tziafas D, Koliniotou-Koumpia E, Tziafa C, Papadimitriou S (2007) Effects of a new antibacterial adhesive on the repair capacity of the pulp-dentine complex in infected teeth. Int Endod J 40:58–66
Van Meerbeek B, Yoshida Y, Inoue S, Vargas S, Abe Y, Fukuda R, Okazaki M, Lambrechts P, Vanherle G (2000) Bonding mechanism and micro-tensile bond strength of a 4-MET-based self-etching adhesive. J Dent Res 79(special issue):249 (abstract #845)
Van Meerbeek B, Vargas S, Inoue S, Yoshida Y, Peumans M, Lambrechts P, Vanherle G (2001) Adhesives and cements to promote preservation dentistry. In: Proceeding from the international symposium on management alternatives for the carious lesions. Oper Dent 26(Suppl 6):119–144
Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, Van Landuyt K, Lambrechts P, Vanherle G (2003) Adhesion to enamel and dentin: current status and future challenges. Buonocore memorial lectured. Oper Dent 28:215–235
Wang Y, Spencer P (2004) Physicochemical interactions at the interfaces between self-etch adhesive systems and dentine. J Dent 32:567–579
Wang Y, Spencer P (2005) Continuing etching of an all-in-one adhesive in wet dentin tubules. J Dent Res 84(4):350–354
Acknowledgments
The present study was partially co-funded by the European Union-European Social Fund and the National Fund Pythagoras-EPEAEK II.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Koliniotou-Koumpia, E., Papadimitriou, S. & Tziafas, D. Pulpal responses after application of current adhesive systems to deep cavities. Clin Oral Invest 11, 313–320 (2007). https://doi.org/10.1007/s00784-007-0121-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00784-007-0121-4