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DNA binding, DNA cleavage and HSA interaction of several metal complexes containing N-(2-hydroxyethyl)-N′-benzoylthiourea and 1,10-phenanthroline ligands

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Abstract

Four novel ternary metal complexes of the type [M(Phen)(L1)2)] [phen = 1,10-phenanthroline, L1 = N-(2-hydroxyethyl)-N′-benzoylthiourea, M = Ni(II)(1), Co(II) (2), Cu(II) (3), Pd(II) (4)] were synthesized. The organic ligands and their corresponding organometallic complexes have been characterized using UV–vis absorption spectroscopy, element analysis, infrared radiation spectroscopy and fluorescence spectra. DNA binding and cleavage studies of these complexes were conducted in detail. In vitro DNA-binding properties were studied by electronic absorption spectra and fluorescence spectra methods. The results indicate that all of the ternary metal complexes can efficiently bind to DNA via intercalation mode. The DNA-binding constants for all ternary compounds are around 4 × 106 M−1. The binding propensity of the complexes to human serum albumin (HSA) was also investigated. Agarose gel electrophoresis study revealed that the metal complexes could cleave super-coiled pBR322 DNA to a nicked form in the absence of external agents. In vitro anti bacterial studies show that copper complex has weak antibacterial activities. Copper complex exhibits a better biological activity among all complexes. This study provides a new perspective and evaluation on the role and importance of the effect factors on the medicinal properties of benzoylthiourea compounds.

Graphical abstract

Synchronous fluorescence spectra of HSA (10 μM) as a function of concentration of the complexes 1–4.

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Abbreviations

HSA:

Human serum albumin

DNA:

Deoxyribose nucleic acid

CT-DNA:

Calf-thymus DNA

DMSO:

Dimethyl sulphoxide

BSA:

Bovine serum albumin

Tris-HCl:

Tris(hydroxymethyl)aminomethane

EB:

Ethidium bromide

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Acknowledgments

The authors would like to thank the National Natural Science Foundation of China (21174114, 21167015), the Innovative Team of Ministry of Education of China (IRT15R56), and “Integration and demonstration of the key technology of industrial capacity promotion for Lanzhou Lily” (2015 technological innovation special fiscal funds of Gansu province) for financial support.

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Authors and Affiliations

  1. Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China

    Bo Peng, Zhuantao Gao, Guorong Chen & Min Zhou

  2. Food Test Center of Jiuquan, Jiuquan, 735000, China

    Tingting Li

  3. College of Life Science, Northwest Normal University, Lanzhou, 730070, China

    Xibo Li & Ji Zhang

Authors
  1. Bo Peng
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  2. Zhuantao Gao
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  3. Xibo Li
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  4. Tingting Li
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  5. Guorong Chen
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  6. Min Zhou
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  7. Ji Zhang
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Correspondence to Bo Peng.

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Peng, B., Gao, Z., Li, X. et al. DNA binding, DNA cleavage and HSA interaction of several metal complexes containing N-(2-hydroxyethyl)-N′-benzoylthiourea and 1,10-phenanthroline ligands. J Biol Inorg Chem 21, 903–916 (2016). https://doi.org/10.1007/s00775-016-1388-1

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  • DOI: https://doi.org/10.1007/s00775-016-1388-1

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