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The metal-binding features of the recombinant mussel Mytilus edulis MT-10-IV metallothionein

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Abstract

In contrast with the paradigmatic mammalian metallothioneins (MTs), mollusc MT systems consist at least of a high-cadmium induced form, possibly involved in detoxification, and another isoform either constitutive or regulated by essential metals and probably associated with housekeeping metabolism. With the aim of providing a deeper characterization of the coordination features of a molluscan MT peptide of the latter kind, we have analyzed here the metal-binding abilities of the recombinant MeMT-10-IV isoform of Mytilus edulis (MeMT). Also, comparison with other MTs of this type has been undertaken. A synthetic complementary DNA was constructed, cloned and expressed into two Escherichia coli systems. Upon zinc coordination, MeMT folds in vivo into highly chiral and stable Zn7 complexes, with an exceptional reluctance to fully substitute cadmium(II) and/or copper(I) for zinc(II). In vivo cadmium binding leads to homometallic Cd7 complexes that structurally differ from any of the in vitro prepared Cd7 complexes. Homometallic Cu–MeMT can only be obtained in vitro from Zn7–MeMT after a great molar excess of copper(I) has been added. In vivo, two different heterometallic Zn,Cu–MeMT complexes are recovered, which nicely correspond to two distinct stages of the in vitro zinc/copper replacement. These MeMT metal-binding features are consistent with a physiological role related to basal/housekeeping metal, mainly zinc, metabolism, and confirm the correspondence between the MeMT gene response pattern and the functional properties of the encoded protein.

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Acknowledgments

This work was supported by Spanish Ministerio de Ciencia y Tecnología grants BIO2006-14420-C02-01 for S.A. and BIO2006-14420-C02-02 for M.C. R.O. received a predoctoral fellowship from the Departament de Química, Universitat Autònoma de Barcelona. We thank the Serveis Científico-Tècnics, Universitat de Barcelona (gas chromatography–flame photometric detection, ICP–AES, ESI–MS, DNA sequencing) and the Servei d’Anàlisi Química (SAQ), Universitat Autònoma de Barcelona (CD, UV–vis) for allocating instrument time.

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Authors and Affiliations

  1. Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain

    Rubén Orihuela, Roger Bofill & Mercè Capdevila

  2. Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028, Barcelona, Spain

    Jordi Domènech & Sílvia Atrian

  3. Biochemisches Institut, Universität Zürich, 8057, Zurich, Switzerland

    Chunhui You, Elaine A. Mackay & Jeremias H. R. Kägi

  4. Institut de Biomedicina, Universitat de Barcelona, 08028, Barcelona, Spain

    Mercè Capdevila & Sílvia Atrian

Authors
  1. Rubén Orihuela
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  2. Jordi Domènech
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  3. Roger Bofill
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  4. Chunhui You
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  5. Elaine A. Mackay
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  6. Jeremias H. R. Kägi
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  7. Mercè Capdevila
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  8. Sílvia Atrian
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Corresponding author

Correspondence to Sílvia Atrian.

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R. Orihuela and J. Domènech contributed equally to this work.

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Orihuela, R., Domènech, J., Bofill, R. et al. The metal-binding features of the recombinant mussel Mytilus edulis MT-10-IV metallothionein. J Biol Inorg Chem 13, 801–812 (2008). https://doi.org/10.1007/s00775-008-0367-6

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  • DOI: https://doi.org/10.1007/s00775-008-0367-6

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