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Effect of whole body vibration on HIF-2α expression in SD rats with early knee osteoarthritis

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Journal of Bone and Mineral Metabolism Aims and scope Submit manuscript

Abstract

Introduction

To investigate the effect of different frequencies of whole body vibration (WBV) on articular cartilage of early knee osteoarthritis (OA) rats and determine whether WBV would influence the pathway of hypoxia-inducible factor-2α (HIF-2α) regulation-related genes after 8 weeks of treatment.

Materials and methods

Forty 8-week-old OA rats were divided into five groups: sham control (SC); high frequency 60 Hz (HV1); high frequency 40 Hz (HV2); middle frequency 20 Hz (MV) and low frequency 10 Hz (LV). WBV (0.3 g) treatment was given 40 min/day and 5 days/week. After 8 weeks, rats were killed and knees were harvested. OA grading score: Osteoarthritis Research Society International (OARSI), and the expression of related genes: interleukin-1β (IL-1β), HIF-2α, matrix metalloproteinases-13 (MMP-13), and collagen type II alpha 1 (COL2A1), at both mRNA and protein levels were analyzed.

Results

After 8 weeks of WBV, our data showed that lower frequency (10 Hz) was more effective than the higher ones, yet they all suggested that WBV alleviates the erosion of knee articular cartilage in early OA. The expression of IL-1β, HIF-2α and MMP-13 decreased with frequency and reached the lowest level at 10 Hz, the expression of COL2A1 increased with frequency and reached the highest level at 10 Hz.

Conclusions

This study demonstrates that WBV could alleviate the degeneration of knee joints in an early OA rat model. WBV regulates related gene expression at both mRNA and protein levels. HIF-2α could be a therapeutic target. The effect of WBV is frequency dependent; the lower frequency shows better effects.

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Acknowledgements

This research was supported in part by Anhui Natural Science Foundation (China) under Award Number 1508085MH181. The content of the work is solely the responsibility of the authors and does not necessarily represent the official views of the funders. The funders had no role in the design, data collection, analysis, and interpretation of data, in the writing of the manuscript or in the decision to submit the manuscript for publication. Funding sources are not associated with the scientific contents of the study. We gratefully acknowledge the cooperation of other participants who took time out of their schedules to help with this project.

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Authors and Affiliations

  1. School of Kinesiology, Shanghai University of Sport, Shanghai, 200438, China

    Lian Wang

  2. Ministry of Science and Education, Anhui Provincial Hospital of Integrated Chinese and Western Medicine (The Third Affiliated Hospital of Anhui University of Chinese Medicine), No. 45, Shihe Road, Wulidun Subdistrict, Shushan District, Hefei, 230061, Anhui Province, China

    Zongbao Wang & Tao Li

  3. Graduate School, Anhui University of Chinese Medicine, Hefei, 230038, Anhui Province, China

    Qiqi Liu

  4. Clinical College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, 230038, Anhui Province, China

    Jingchao Su & Tianming Wang

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  1. Lian Wang
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  2. Zongbao Wang
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Contributions

ZBW oversaw the project; LW and ZBW designed the study; LW and QQL conducted the experiments; JCS and TMW analyzed and interpreted data; LW and TL participated in drafting the main manuscript text; ZBW and TMW revised the article critically. All authors contributed to writing or reviewing the manuscript and final approval.

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Correspondence to Zongbao Wang.

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All authors have no conflicts of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This research was approved by the Ethics Committee of Animal Experiments of Anhui University of Chinese Medicine.

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This study does not involve human participants and, therefore, does not require informed consent.

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Wang, L., Wang, Z., Liu, Q. et al. Effect of whole body vibration on HIF-2α expression in SD rats with early knee osteoarthritis. J Bone Miner Metab 38, 491–500 (2020). https://doi.org/10.1007/s00774-020-01092-3

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  • DOI: https://doi.org/10.1007/s00774-020-01092-3

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