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Effects of bazedoxifene and low-intensity aerobic exercise on bone and fat parameters in ovariectomized rats

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Abstract

Introduction

Postmenopausal osteoporosis and dyslipidemia are well-known skeletal and metabolic changes in middle-aged women. We investigated the effects of combined treatments with a selective estrogen receptor modulator (SERM) and exercise on bone and fat parameters in ovariectomized (OVX) rats.

Materials and Methods

Sixteen-week-old female Sprague–Dawley rats underwent bilateral ovariectomy, and rats were randomized to BZA (bazedoxifene at 0.3 mg/kg/day), Exe (treadmill exercise at 12–15 m/min, 60 min/day, 5 days/week), Comb (BZA and Exe), and Cont (control treated with vehicle and no exercise) groups 8 weeks after ovariectomy. After 4 or 8 weeks of treatment, bone mineral density (BMD) of the total femur and lumbar spine and whole-body percentage fat mass were determined by dual-energy X-ray absorptiometry, and mechanical testing of the femoral shaft, and bone and fat histomorphometric analyses of the proximal tibia were performed.

Results

Treadmill exercise had decreased bone marrow adipocytes from 4 weeks of treatment and whole-body percentage fat mass at 8 weeks. BZA increased BMD at the lumbar spine and decreased the whole-body percentage fat mass from 4 weeks and bone marrow adipocytes at 8 weeks. Combination therapy increased BMD for the lumbar spine and decreased bone marrow adipocytes and whole-body percentage fat mass from 4 weeks.

Conclusion

Combination therapy with BZA and exercise appears effective to improve bone and fat parameters in OVX rats.

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Acknowledgements

The authors would like to thank Ms. Matsuzawa and Ms. Kudo for their support in performing the experiments.

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Authors and Affiliations

  1. Department of Orthopedic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan

    Yusuke Yuasa, Naohisa Miyakoshi, Yuji Kasukawa, Itsuki Nagahata, Manabu Akagawa, Yuichi Ono, Chiaki Sato, Hiroyuki Tsuchie, Koji Nozaka, Hiroyuki Nagasawa, Michio Hongo & Yoichi Shimada

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  1. Yusuke Yuasa
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  2. Naohisa Miyakoshi
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Correspondence to Naohisa Miyakoshi.

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All the authors have no conflict of interest.

Ethical approval

The protocols for all animal experiments were approved in advance by the Animal Research Committee of our institute, and all subsequent animal experiments adhered to the “Guidelines for Animal Experimentation” of our university (approval number: a-1-2827).

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Yuasa, Y., Miyakoshi, N., Kasukawa, Y. et al. Effects of bazedoxifene and low-intensity aerobic exercise on bone and fat parameters in ovariectomized rats. J Bone Miner Metab 38, 179–187 (2020). https://doi.org/10.1007/s00774-019-01045-5

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