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Histological examination of bone regeneration achieved by combining grafting with hydroxyapatite and thermoplastic bioresorbable plates

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Abstract

In this study, we present a novel guided bone regeneration (GBR) concept that consists of combining Boneject, a bone substitute containing atelocollagen and bovine hydroxyapatite particles, with thermoplastic, bioresorbable plates (DeltaSystem) known to resist mechanical loading. In rat calvariae, standardized bone defects were filled with Boneject and covered with a convex DeltaSystem plate. Tissue from rats at 1, 2, 4, 8, and 12 weeks postoperation were fixed with an aldehyde solution, and the new bone formed at the defects was histologically assessed. At 1 week, alkaline phosphatase (ALP)-negative cells deriving from the bottom region of the defect could be found up to half the height of the cavity. Boneject particle surfaces in the bottom region revealed an intense osteopontin immunopositivity whereas those in the upper region did not. Tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts accumulated on the surfaces of osteopontin-coated particles. A newly formed, woven-like bone featuring ALP-positive osteoblasts extended from the native bone. At the second week, the newly formed woven bone had surrounded the Boneject particles. Cement lines, which indicate active bone remodeling, could be observed in the new bone despite its immaturity. Four weeks after surgery, the new bone had reached the height of the DeltaSystem plate, and just beneath it a periostin-positive fibrous layer covered the mix of new bone and Boneject particles. By then, despite having acceptable histological features, electron probe microanalyzer (EPMA) and transmission electron microscope (TEM) analyses revealed that the new bone could not be regarded as compact bone. At 8 and 12 weeks, the new bone showed compact bone-like features according to TEM and EPMA assessments. Summarizing, the combination of a bone substitute such as Boneject and a rigid, bioresorbable plate appears to be osteoconductive and to promote bone remodeling, leading to the genesis of a tissue similar to the one that is regarded as the “gold standard” for bone regeneration: the compact bone.

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Authors and Affiliations

  1. Division of Reconstructive Surgery for Oral and Maxillofacial Region, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Taku Kojima, Michiko Yoshizawa & Chikara Saito

  2. Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Taku Kojima, Akiko Suzuki & Takeyasu Maeda

  3. Division of Oral and Maxillofacial Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Paulo Henrique Luiz de Freitas

  4. Center for Transdisciplinary Research, Niigata University, 2-5274 Gakkocho-dori, Niigata, 951-8514, Japan

    Taku Kojima, Norio Amizuka, Paulo Henrique Luiz de Freitas & Takeyasu Maeda

  5. Department of Biological Information, Tokyo Institute of Technology, Yokohama, Japan

    Akira Kudo

Authors
  1. Taku Kojima
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  2. Norio Amizuka
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  3. Akiko Suzuki
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  4. Paulo Henrique Luiz de Freitas
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  5. Michiko Yoshizawa
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  6. Akira Kudo
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  7. Chikara Saito
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  8. Takeyasu Maeda
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Correspondence to Norio Amizuka.

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Kojima, T., Amizuka, N., Suzuki, A. et al. Histological examination of bone regeneration achieved by combining grafting with hydroxyapatite and thermoplastic bioresorbable plates. J Bone Miner Metab 25, 361–373 (2007). https://doi.org/10.1007/s00774-007-0763-y

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  • DOI: https://doi.org/10.1007/s00774-007-0763-y

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