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Antidiabetic activity of Tartary buckwheat protein-derived peptide AFYRW and its effects on protein glycosylation of pancreas in mice

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Abstract

Diabetes Mellitus (DM) is one of the most important public health problems, and new antidiabetic drugs with fewer side effects are urgently needed. Here, we measured the antidiabetic effects of an antioxidant peptide (Ala-Phe-Tyr-Arg-Trp, AFYRW) from Tartary Buckwheat Albumin (TBA) in a high-fat diet/streptozotocin (HFD/STZ)-induced diabetic mouse model. The data showed that AFYRW suppressed hepatocyte steatosis and triglycerides while ameliorating insulin resistance in mice. Successively, the influence of AFYRW on aberrant protein glycosylation in diabetic mice was further investigated by lectin microarrays. The results suggested AFYRW could restore the expression of GalNAc, GalNAcα1-3Gal and GalNAcα1-3Galβ1-3/4Glc recognized by PTL-I, Siaα2-3Galβ1-4Glc(NAc)/Glc, Siaα2-3Gal, Siaα2-3 and Siaα2-3GalNAc recognized by MAL-II, terminating in GalNAcα/β1-3/6Gal recognized by WFA and αGalNAc, αGal, anti-A and B recognized by GSI-I to normal levels in the pancreas of HFD-STZ-induced diabetic mice. This work may provide new targets for the future discovery of potential biomarkers to evaluate the efficacy of food-derived antidiabetic drugs based on precise alterations of glycopatterns in DM.

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Data availability

The data used in this work are available upon request.

Abbreviations

AFYRW:

Ala-Phe-Tyr-Arg-Trp

DM:

Diabetes mellitus

FBG:

Fasting blood glucose

FINS:

Fasting insulin

HFD:

High-fat diet

HOMA-IR:

Insulin resistance index

LDL:

Low-density lipoprotein

ND:

Normal diet

NFI:

Normalized fluorescence intensity

OGTT:

Oral glucose tolerance test

STZ:

Streptozotocin

TBA:

Tartary buckwheat albumin

T2DM:

Type 2 diabetes

TG:

Triglyceride

TC:

Total cholesterol

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Funding

This work was supported by Guizhou Provincial Science and Technology Projects (Grant No. 19NSP038; ZK [2021] 104) and the Research Foundation for Advanced Talents of Guizhou Medical University (Grant No. University Contract of Doctors J [2022] 29).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Guizhou Medical University, Guiyang, 550004, People’s Republic of China

    Jiajun Yang, Jie Zuo, Yan Deng, Lilin Zhang & Hongmei Li

  2. Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education & Key Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical University, Guiyang, 550004, Guizhou, People’s Republic of China

    Jiajun Yang & Hongmei Li

  3. Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, 710069, People’s Republic of China

    Hanjie Yu, Chen Zhang & Zheng Li

Authors
  1. Jiajun Yang
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  2. Jie Zuo
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  3. Yan Deng
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  4. Lilin Zhang
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  5. Hanjie Yu
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  6. Chen Zhang
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  7. Zheng Li
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  8. Hongmei Li
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Contributions

JJY, JZ and HML conceived and designed experiments. JJY, JZ, YD, LLZ, HJY, CZ and ZL designed and performed the experiments. JJY, JZ and HML designed and performed the data acquisition and analysis. ZL helped with critical advice and discussion. JJY, ZL and HML prepared the manuscript. All authors approved the final manuscript.

Corresponding authors

Correspondence to Zheng Li or Hongmei Li.

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Conflict of interest

The authors declare no competing interests.

Ethical approval

All mice experiments were performed in compliance with the People's Republic of China Legislation Regarding the Use and Care of Laboratory.

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Handling editor: S. Albrecht.

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Yang, J., Zuo, J., Deng, Y. et al. Antidiabetic activity of Tartary buckwheat protein-derived peptide AFYRW and its effects on protein glycosylation of pancreas in mice. Amino Acids 55, 1063–1071 (2023). https://doi.org/10.1007/s00726-023-03294-1

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  • DOI: https://doi.org/10.1007/s00726-023-03294-1

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