Summary.
Handling and detoxification of metals by enzymes is a major issue that is not in the focus of current biomedical research concepts. The finding of the presence of arsenic (+3 Oxidation State) methyltransferase (AS3MT) in neuroblastoma cells NE-115 as a high abundance protein made us investigate primary structure of AS3MT reflecting an example of metal-handling in eucaryotes. Proteins extracted from NE-115 cells were run on 2-DE followed by two different mass spectrometrical methods. High sequence coverage was obtained by multiple protease digestion and a sequence conflict was solved at arginine 335.
These findings are important when future studies on this enzyme are designed at the protein level and in particular, when antibodies against this protein will be generated.
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References
MM Bradford (1976) ArticleTitleA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248–254 Occurrence Handle942051 Occurrence Handle10.1016/0003-2697(76)90527-3 Occurrence Handle1:CAS:528:DyaE28XksVehtrY%3D
F Challenger (1951) ArticleTitleBiological methylation Adv Enzymol 12 429–491 Occurrence Handle1:CAS:528:DyaG38XitVOrtA%3D%3D
WQ Chen SU Kang G Lubec (2006) ArticleTitleProtein profiling by the combination of two independent mass spectrometry techniques Nat Protoc 1 1446–1452 Occurrence Handle17406433 Occurrence Handle10.1038/nprot.2006.246 Occurrence Handle1:CAS:528:DC%2BD28Xht1eitL7I
EA Crecelius (1977) ArticleTitleChanges in the chemical speciation of arsenic following ingestion by man Environ Health Perspect 19 147–150 Occurrence Handle908293 Occurrence Handle10.2307/3428467 Occurrence Handle1:CAS:528:DyaE2sXlvFGisrk%3D
WR Cullen BC McBride J Reglinski (1984) ArticleTitleThe reduction of trimethylarsineoxide to trimethylarsine by thiols: a mechanistic model for the biological reduction of arsenicals J Inorg Biochem 21 45–60 Occurrence Handle10.1016/0162-0134(84)85038-2 Occurrence Handle1:CAS:528:DyaL2cXkvFWisrk%3D
DS Gerhard L Wagner EA Feingold CM Shenmen LH Grouse G Schuler SL Klein S Old R Rasooly P Good M Guyer AM Peck JG Derge D Lipman FS Collins W Jang S Sherry M Feolo L Misquitta E Lee K Rotmistrovsky SF Greenhut CF Schaefer K Buetow TI Bonner D Haussler J Kent M Kiekhaus T Furey M Brent C Prange K Schreiber N Shapiro NK Bhat RF Hopkins F Hsie T Driscoll MB Soares TL Casavant TE Scheetz MJ Brown-stein TB Usdin S Toshiyuki P Carninci Y Piao DB Dudekula MS Ko K Kawakami Y Suzuki S Sugano CE Gruber MR Smith B Simmons T Moore R Waterman SL Johnson Y Ruan CL Wei S Mathavan PH Gunaratne J Wu AM Garcia SW Hulyk E Fuh Y Yuan A Sneed C Kowis A Hodgson DM Muzny J McPherson RA Gibbs J Fahey E Helton M Ketteman A Madan S Rodrigues A Sanchez M Whiting A Madari AC Young KD Wetherby SJ Granite PN Kwong CP Brinkley RL Pearson GG Bouffard RW Blakesly ED Green MC Dickson AC Rodriguez J Grimwood J Schmutz RM Myers YS Butterfield M Griffith OL Griffith MI Krzywinski N Liao R Morin D Palmquist AS Petrescu U Skalska DE Smailus JM Stott A Schnerch JE Schein SJ Jones RA Holt A Baross MA Marra S Clifton KA Makowski S Bosak J Malek InstitutionalAuthorNameMGC Project Team (2004) ArticleTitleThe status, quality, and expansion of the NIH full-length cDNA project: the mammalian gene collection (MGC) Genome Res 14 2121–2127 Occurrence Handle15489334 Occurrence Handle10.1101/gr.2596504
T Hayakawa Y Kobayashi X Cui S Hirano (2005) ArticleTitleA new metabolic pathway of arsenite: arsenite-glutathione complexes are substrates for human arsenic methyltransferase Cyt19 Arch Toxicol 79 183–191 Occurrence Handle15526190 Occurrence Handle10.1007/s00204-004-0620-x Occurrence Handle1:CAS:528:DC%2BD2MXjtlehtr8%3D
SM Healy EA Casarez F Ayala-Fierro H Aposhian (1998) ArticleTitleEnzymatic methylation of arsenic compounds. V. Arsenite methyltransferase activity in tissues of mice Toxicol Appl Pharmacol 148 65–70 Occurrence Handle9465265 Occurrence Handle10.1006/taap.1997.8306 Occurrence Handle1:CAS:528:DyaK1cXnvFWisQ%3D%3D
S Lin Q Shi FB Nix M Styblo MA Beck KM Herbin-Davis LL Hall JB Simeonsson DJ Thomas (2002) ArticleTitleA novel S-adenosyl-L-methionine: arsenic(III) methyltransferase from rat liver cytosol J Biol Chem 29 10795–10803 Occurrence Handle10.1074/jbc.M110246200
R Mukhopadhyay BP Rosen (2002) ArticleTitleArsenate reductases in prokaryotes and eukaryotes Environ Health Perspect 110 745–748 Occurrence Handle12426124 Occurrence Handle1:CAS:528:DC%2BD38XovFKhs7k%3D
JE Oh K Krapfenbauer M Fountoulakis T Frischer G Lubec (2004) ArticleTitleEvidence for the existence of hypothetical proteins in human bronchial epithelial, fibroblast, amnion, lymphocyte, mesothelial and kidney cell lines Amino Acids 26 9–18 Occurrence Handle14752611 Occurrence Handle10.1007/s00726-003-0060-6 Occurrence Handle1:CAS:528:DC%2BD2cXnsFGmsA%3D%3D
JE Oh K Karlmark Raja JH Shin M Hengstschläger A Pollak G Lubec (2005) ArticleTitleThe neuronal differentiation process involves a series of antioxidant proteins Amino Acids 29 273–282 Occurrence Handle15986180 Occurrence Handle10.1007/s00726-005-0214-9 Occurrence Handle1:CAS:528:DC%2BD2MXht1OqsLnK
JE Oh K Karlmark Raja JH Shin A Pollak M Hengstschlager G Lubec (2006) ArticleTitleCytoskeleton changes following differentiation of N1E-115 neuroblastoma cell line Amino Acids 31 289–298 Occurrence Handle16547650 Occurrence Handle10.1007/s00726-005-0256-z Occurrence Handle1:CAS:528:DC%2BD28XhtVKjtLvE
Phillips et al. (1999) Genebank submission, available under (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=6006812)
D Suckau A Resemann M Schuerenberg P Hufnagel (2003) ArticleTitleA novel MALDI LIFT-TOF/TOF mass spectrometer for proteomics Anal Bioanal Chem 376 952–965 Occurrence Handle12830354 Occurrence Handle10.1007/s00216-003-2057-0 Occurrence Handle1:CAS:528:DC%2BD3sXmtVGrsr8%3D
FS Walton SB Waters SL Jolley EL LeCluyse DJ Thomas M Styblo (2003) ArticleTitleSelenium compounds modulate the activity of recombinant rat AsIII-methyltransferase and the methylation of arsenite by rat and human hepatocytes Chem Res Toxicol 16 261–265 Occurrence Handle12641425 Occurrence Handle10.1021/tx025649r Occurrence Handle1:CAS:528:DC%2BD3sXht1Oht74%3D
SB Waters M Styblo DJ Thomas (2004) ArticleTitleEndogenous reductants support the catalytic function of recombinant rat cyt19, an arsenic methyltransferase Chem Res Toxicol 17 404–409 Occurrence Handle15025511 Occurrence Handle10.1021/tx0342161 Occurrence Handle1:CAS:528:DC%2BD2cXht1aiu70%3D
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Authors’ address: Prof. Gert Lubec, Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18, A-1090 Vienna, Austria
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John, J., Oh, JE., Pollak, A. et al. Identification and characterisation of arsenite (+3 Oxidation State) methyltransferase (AS3MT) in mouse neuroblastoma cell line N1E-115. Amino Acids 35, 355–358 (2008). https://doi.org/10.1007/s00726-007-0613-1
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DOI: https://doi.org/10.1007/s00726-007-0613-1