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Rotational Correlation Time Studies on Nitroxyl Radicals Using 300 MHz ESR Spectrometer in High Viscous Liquid

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Abstract

The mobility studies on 14N-labeled TEMPONE, TEMPO, carbamoyl-PROXYL, carboxy-PROXYL in high viscous liquid were carried out on a 300 MHz electron spin resonance (ESR) spectrometer. The ESR parameters, such as a line width, signal intensity ratio, g-factor, hyperfine coupling constant and correlation time, were determined. The line width broadening increases twofold in high viscous samples of 14N-labeled carbamoyl-PROXYL and carboxy-PROXYL, but this line broadening is negligibly small in the high viscous sample (85% glycerol) of 14N-labeled TEMPO. The correlation time also increases (~30 times) in the high viscous sample (85% glycerol) of 14N-labeled carbamoyl-PROXYL and carboxy-PROXYL, but there is no considerable increase in the high viscous sample of 14N-labeled TEMPO. TEMPONE has the narrowest line width and is also highly sensitive to viscosity. The correlation time increases (~13 times) in the high viscous sample (85% glycerol) of 14N-labeled TEMPONE. Therefore, this study reveals that the 14N-labeled TEMPONE radical is the most suitable spin probe for in vivo studies in high viscous biological fluids.

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Acknowledgments

We thank our college management for their constant encouragement. This work was supported by the University Grants Commission (UGC), New Delhi (major research project: F.No.38-140/2009(SR)).

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Authors and Affiliations

  1. Department of Physics, NMSSVN College, Nagamalai, Madurai, 625 019, Tamilnadu, India

    A. Milton Franklin Benial & M. Kumara Dhas

  2. Department of Chemistry, NMSSVN College, Nagamalai, Madurai, 625 019, Tamilnadu, India

    A. Jawahar

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  1. A. Milton Franklin Benial
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Correspondence to A. Milton Franklin Benial.

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Milton Franklin Benial, A., Kumara Dhas, M. & Jawahar, A. Rotational Correlation Time Studies on Nitroxyl Radicals Using 300 MHz ESR Spectrometer in High Viscous Liquid. Appl Magn Reson 40, 311–319 (2011). https://doi.org/10.1007/s00723-011-0220-x

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  • DOI: https://doi.org/10.1007/s00723-011-0220-x

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