Skip to main content
SpringerLink
Log in

Application of NMR Spectroscopy and Imaging in Heterogeneous Biocatalysis

  • Published:
Applied Magnetic Resonance Aims and scope Submit manuscript

Abstract

Heterogeneously catalyzed enzymatic glucose isomerization was considered as a model process to extend the application of nuclear magnetic resonance (NMR) and magnetic resonance imaging techniques to the studies of biocatalytic processes and heterogeneous biocatalysts. It has been demonstrated that the T 2 times of glucose are different for its aqueous solution in the pores of an unmodified porous support and in a heterogeneous biocatalyst, comprising bacterial cells immobilized on the same support. This observation has been used to map the spatial distribution of the active component within a packed bed of biocatalyst in a model reactor. 13C NMR spectroscopy was applied to follow the progress of glucose isomerization catalyzed by the heterogeneous biocatalyst in a batch reactor. The utilization of proton spin decoupling and nuclear Overhauser effect was shown to be necessary to obtain high signal-to-noise ratio in the natural abundance 13C NMR spectra of a glucose–fructose syrup present in the packed bed of biocatalyst. The spectra thus obtained were suitable for the quantification of the glucose-to-fructose ratio achieved in the biocatalytic reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. M. Held, A. Schmidt, J.B. van Beilen, B. Witholt, Pure Appl. Chem. 72, 1337–1343 (2000)

    Article  Google Scholar 

  2. H.O. Jauregui, N.R. Chowdhury, J.R. Chowdhury, Cell Transplant. 5, 353–367 (1996)

    Article  Google Scholar 

  3. I.V. Koptyug, A.V. Kulikov, A.A. Lysova, V.A. Kirillov, V.N. Parmon, R.Z. Sagdeev, J. Am. Chem. Soc. 124, 9684–9685 (2002)

    Article  Google Scholar 

  4. I.V. Koptyug, A.A. Lysova, A.V. Kulikov, V.A. Kirillov, V.N. Parmon, R.Z. Sagdeev, Appl. Catal. A Gen. 267, 143–148 (2004)

    Article  Google Scholar 

  5. I.V. Koptyug, A.A. Lysova, in Nuclear Magnetic Resonance Imaging in Chemical Engineering, ed. by S. Stapf, S. Han (Wiley-VCH, Weinheim, 2005), pp. 570–589.

  6. I.V. Koptyug, A.V. Khomichev, A.A. Lysova, R.Z. Sagdeev, J. Am. Chem. Soc. 130, 10452–10453 (2008)

    Article  Google Scholar 

  7. L.F. Gladden, M.D. Mantle, A.J. Sederman, Adv. Catal. 50, 1–75 (2006)

    Article  Google Scholar 

  8. J.D. Seymour, S.L. Codd, E.L. Gjersing, P.S. Steward, J. Magn. Reson. 167, 322–327 (2004)

    Article  ADS  Google Scholar 

  9. E.L. Gjersing, S.L. Codd, J.D. Seymour, P.S. Steward, Biotechnol. Bioeng. 89, 822–834 (2005)

    Article  Google Scholar 

  10. K. Potter, R.L. Kleinberg, F.J. Brockman, E.W. McFarland, J. Magn. Reson. B 113, 9–15 (1996)

    Article  Google Scholar 

  11. B.C. Hoskins, L. Fevang, P.D. Majors, M.M. Sharma, G. Georgiou, J. Magn. Reson. 139, 67–73 (1999)

    Article  ADS  Google Scholar 

  12. H. Van As, P. Lens, J. Ind. Microbiol. Biotechnol. 26, 43–52 (2001)

    Article  Google Scholar 

  13. K.P. Nott, M. Paterson-Beedle, L.E. Macaskie, L.D. Hall, Biotechnol. Lett. 23, 1749–1757 (2001)

    Article  Google Scholar 

  14. D.A. Graf von der Schulenburg, D.J. Holland, M. Paterson-Beedle, L.E. Macaskie, L.F. Gladden, M.L. Johns, Biotechnol. Bioeng. 99, 821–829 (2008)

    Article  Google Scholar 

  15. E.E. Beuling, D. van Dusschoten, P. Lens, J.C. van den Heuvel, H. Van As, P.P. Ottengraf, Biotechnol. Bioeng. 60, 283–291 (1998)

    Article  Google Scholar 

  16. M. Vogt, H.-C. Flemming, W.S. Veeman, J. Biotechnol. 77, 137–146 (2000)

    Article  Google Scholar 

  17. C. Mayer, R. Moritz, C. Kirschner, W. Borchard, R. Maibaum, J. Wingender, H.-C. Flemming, Int. J. Biol. Macromol. 26, 3–16 (1999)

    Article  Google Scholar 

  18. C. Mayer, D. Lattner, N. Schurks, J. Ind. Microbiol. Biotechnol. 26, 62–69 (2001)

    Article  Google Scholar 

  19. Z. Lewandowski, S.A. Altobelli, P.D. Majors, E. Fukushima, Water Sci. Technol. 26, 577–584 (1992)

    Google Scholar 

  20. B. Manz, F. Volke, D. Goll, H. Horn, Biotechnol. Bioeng. 84, 424–432 (2003)

    Article  Google Scholar 

  21. P.D. Majors, J.S. McLean, G.E. Pinchuk, J.K. Fredrickson, Y.A. Gorby, K.R. Minard, R.A. Wind, J. Microbiol. Meth. 62, 337–344 (2005)

    Article  Google Scholar 

  22. M. Kueppers, C. Heine, S. Han, S. Stapf, B. Bluemich, Appl. Magn. Reson. 22, 235–246 (2002)

    Article  Google Scholar 

  23. G.A. Kovalenko, L.V. Perminova, T.G. Terentieva, L.I. Sapunova, A.G. Lobanok, T.V. Chuenko, N.A. Rudina, E.I. Chernyak, Appl. Biochem. Microbiol. 44, 174–181 (2008)

    Google Scholar 

  24. L.V. Perminova, G.A. Kovalenko, N.A. Rudina, L.I. Sapunova, I.O. Tamkovich, A.G. Lobanok, Appl. Biochem. Microbiol. 45(4), 389–394 (2009)

    Google Scholar 

  25. S.J. Angyal, Aust. J. Chem. 21, 2737–2746 (1968)

    Google Scholar 

  26. R.S. Shallenberger, Pure Appl. Chem. 50, 1409–1420 (1978)

    Article  Google Scholar 

  27. Research Information Database of the International Institute of Advanced Industrial Science and Technology. http://www.aist.go.jp/

  28. N. Shiomi, S. Onodera, Agric. Biol. Chem. 52, 2347–2348 (1988)

    Google Scholar 

  29. C.H. Bartholomew, R.J. Farrauto, Fundamentals of Industrial Catalytic Processes (Wiley-Interscience, New York, 2006)

    Google Scholar 

  30. A.M. Dehkordi, I. Safari, M.M. Karima, AIChE J. 54, 1333–1343 (2008)

    Article  Google Scholar 

Download references

Acknowledgments

A.A.L. and I.V.K. thank the Russian Academy of Sciences (grant nr. 5.1.1), the Siberian Branch of the Russian Academy of Sciences (integration grant nrs. 67 and 88), the Russian Foundation for Basic Research (grant nrs. 08-03-00539, 08-03-00661, 08-03-91102) and the programs of support of leading scientific schools (grant nr. NSh-3604.2008.3). A.A.L. acknowledges the Council on Grants of the President of the Russian Federation (MK-5135.2007.3).

Author information

Author notes

  1. Irina I. Koptyug

    Present address: Campus Polytechnique, Institut d’Optique, Palaiseau, France

Authors and Affiliations

  1. International Tomography Center SB RAS, 3A Institutskaya St., 630090, Novosibirsk, Russia

    Irina I. Koptyug, Anna A. Lysova & Igor V. Koptyug

  2. Boreskov Institute of Catalysis SB RAS, 630090, Novosibirsk, Russia

    Anna A. Lysova, Galina A. Kovalenko & Larisa V. Perminova

  3. Novosibirsk State University, 630090, Novosibirsk, Russia

    Anna A. Lysova & Galina A. Kovalenko

Authors
  1. Irina I. Koptyug
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anna A. Lysova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Galina A. Kovalenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Larisa V. Perminova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Igor V. Koptyug
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Igor V. Koptyug.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koptyug, I.I., Lysova, A.A., Kovalenko, G.A. et al. Application of NMR Spectroscopy and Imaging in Heterogeneous Biocatalysis. Appl Magn Reson 37, 483–495 (2010). https://doi.org/10.1007/s00723-009-0074-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00723-009-0074-7

Keywords

Search

Navigation