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Seedling development in maize cv. B73 and blue light-mediated proteomic changes in the tip vs. stem of the coleoptile

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Abstract

In 2009, the draft genome of the reference inbred line of maize (Zea mays L. spp. mays cv. B73) was published so that, using this specific corn variety, molecular analyses of physiological processes became possible. However, the morphology and developmental patterns of B73 maize, compared with that of the more frequently used hybrid varieties, have not yet been analyzed. Here, we describe organ development in seedlings of B73 maize and in those of six other hybrid cultivars, and document significant morphological as well as quantitative differences between these varieties of Z. mays. In a second set of experiments, we used etiolated seedlings of B73 maize to analyze the effect of blue light (BL) on the patterns of proteins in the tip vs. growing region of this sheath-like organ. By using two-dimensional difference gel electrophoresis (2D DIGE), coupled with tandem mass spectrometry, we detected, in the microsomal fraction of maize coleoptile tips, rapid changes in the abundance of protein spots of maize phototropin 1 and several metabolic enzymes. In the sub-apical (growing) region of the coleoptile, proteomic changes were less pronounced. These results suggest that the tip of the coleoptile of B73 maize may serve as a unique model system for dissecting BL responses in a light-sensitive plant organ of known function.

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Acknowledgments

We thank Dr. W. R. Briggs for advice and consultation and the Alexander von Humboldt-Stiftung (AvH, Bonn, Germany) for financial support (AvH-Fellowships Stanford 2009/14 to U. K., Institute of Biology, University of Kassel, Germany). This project was supported by grants from the US National Institute of Health (NIH) (R01GM066258 to Z.-Y. W.), and State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control (2015-cxzt-18 to Z.D.).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Zhiping Deng

  2. Department of Plant Biology, Carnegie Institution for Science, Stanford, California, 94305, USA

    Zhiping Deng, Zhi-Yong Wang & Ulrich Kutschera

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  1. Zhiping Deng
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  2. Zhi-Yong Wang
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  3. Ulrich Kutschera
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Correspondence to Ulrich Kutschera.

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Handling Editor: Peter Nick

Dedicated to the memory of Peter Sitte (1929–2015)

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Deng, Z., Wang, ZY. & Kutschera, U. Seedling development in maize cv. B73 and blue light-mediated proteomic changes in the tip vs. stem of the coleoptile. Protoplasma 254, 1317–1322 (2017). https://doi.org/10.1007/s00709-016-1023-6

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  • DOI: https://doi.org/10.1007/s00709-016-1023-6

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