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Chloramphenicol acetyltransferase—a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae

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Abstract

In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae—a new model organism, which offers unique opportunities for studding the mitochondrial and plastid physiology as well as various evolutionary, structural, and functional features of the photosynthetic apparatus.

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Fig. 1

Abbreviations

PEG:

Polyethylene glycol

Cm:

Chloramphenicol

Chl a :

Chlorophyll a

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Acknowledgments

This investigation was financed by grant Opus 5 (DEC-2013/09/B/NZ1/00187) awarded by the Polish National Science Centre.

We acknowledge the contribution of Dr. Bohdan Paterczyk from the Laboratory of Electron and Confocal Microscopy, University of Warsaw, Poland.

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Authors and Affiliations

  1. Warsaw University, Warsaw, Poland

    Maksymilian Zienkiewicz, Tomasz Krupnik, Anna Drożak, Anna Golke & Elżbieta Romanowska

Authors
  1. Maksymilian Zienkiewicz
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  2. Tomasz Krupnik
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  3. Anna Drożak
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  4. Anna Golke
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  5. Elżbieta Romanowska
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Correspondence to Maksymilian Zienkiewicz.

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Zienkiewicz, M., Krupnik, T., Drożak, A. et al. Chloramphenicol acetyltransferase—a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae . Protoplasma 254, 587–596 (2017). https://doi.org/10.1007/s00709-015-0936-9

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  • DOI: https://doi.org/10.1007/s00709-015-0936-9

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