Summary
A potyvirus, which we call ceratobium mosaic virus, has been detected in about one third of more than 100 plants representing c. 33 orchid genera in two collections in Australia. It was detected using RT-PCR with redundant primers that are Potyviridae-specific and have additional sequences corresponding to either the SP6 or T7 bacteriophage promoters at their 5′-termini. Thus the nucleotide sequence of the resulting PCR fragments, consisting of about 1.7 kb of the 3′ portion of the viral genome, could be determined directly. Viral sequences obtained from five infected orchids indicate that they contained different isolates of a single potyvirus species most closely related to the bean common mosaic group of potyviruses, but clearly distinct from all whose virion protein genes have been reported to the international gene sequence databases.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Accepted December 18, 1997 Received September 9, 1997
Rights and permissions
About this article
Cite this article
Mackenzie, A.M., Nolan, M., Wei, KJ. et al. Ceratobium mosaic potyvirus: another virus from orchids. Arch. Virol. 143, 903–914 (1998). https://doi.org/10.1007/s007050050341
Published:
Issue Date:
DOI: https://doi.org/10.1007/s007050050341