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Identification and molecular characterization of a novel cytorhabdovirus from rose plants (Rosa chinensis Jacq.)

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Abstract

A novel negative-sense single-stranded RNA virus, tentatively named "rose-associated cytorhabdovirus" (RaCV), was identified by high-throughput sequencing. RaCV is 16,067 nucleotides in length and contains eight open reading frames (ORFs 1-8) encoding a nucleocapsid protein (N), a putative phosphoprotein (P), a putative P3 protein (P3), a putative P4 protein (P4), a putative matrix protein (M), a glycoprotein (G), a putative P7 protein (P7), and an RNA-dependent RNA polymerase (L), respectively. The coding genes are flanked by a 3′ leader sequence (228 nt) and a 5′ trailer sequence (251 nt) and are separated by conserved intergenic junctions (3′-AUUCUUUUUG(N)nCUN-5′). Phylogenetic analysis showed that RaCV clustered with yerba mate virus A (YmVA) within the cytorhabdovirus clade, and it exhibited low a degree of nt sequence similarity (<40% identity) to other rhabdoviruses. Amino acid sequence comparisons between the putative proteins of RaCV and the corresponding proteins of other cytorhabdoviruses showed that the sequence identity levels were far below the species demarcation cutoff of 80% for cytorhabdoviruses. These results suggest that RaCV should be classified as a new member of the genus Cytorhabdovirus.

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All relevant data are openly available in the National Center for Biotechnology Information, and within the manuscript and its additional files.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (32072389), the Innovation Research 2035 Pilot Plan of Southwest University (SWU-XDPY22002), the National Key R&D Program of China (2019YFD1001800), the 111 project (B18044), the Chongqing Postgraduate Research and Innovation Project (CYS22259), the National Training Program of Innovation and Entrepreneurship for Undergraduates (202210635047), and the Municipal Training Program of Innovation and Entrepreneurship for Undergraduates (S202210635179).

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Authors and Affiliations

  1. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Yujiao Wu, Mengxue Yang, Han Yang, Yuanjian Qiu, Zhiyou Xuan, Fei Xing & Mengji Cao

  2. Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China

    Yujiao Wu, Mengxue Yang, Han Yang, Yuanjian Qiu, Zhiyou Xuan, Fei Xing & Mengji Cao

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  1. Yujiao Wu
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  2. Mengxue Yang
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Correspondence to Mengji Cao.

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Wu, Y., Yang, M., Yang, H. et al. Identification and molecular characterization of a novel cytorhabdovirus from rose plants (Rosa chinensis Jacq.). Arch Virol 168, 118 (2023). https://doi.org/10.1007/s00705-023-05742-5

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  • DOI: https://doi.org/10.1007/s00705-023-05742-5

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