Abstract
The complete genome sequence of a double-stranded RNA (dsRNA) virus, Rhizoctonia solani dsRNA virus 11 (RsRV11), isolated from Rhizoctonia solani AG-1 IA strain 9-11 was determined. The RsRV11 genome is 9,555 bp in length and contains three conserved domains: structural maintenance of chromosomes (SMC) superfamily, phosphoribulokinase (PRK), and RNA-dependent RNA polymerase (RdRp). The RsRV11 genome has two non-overlapping open reading frames (ORFs). ORF1 is predicted to encode a 204.12-kDa protein that shares low but significant amino acid sequence similarity with a putative protein encoded by Rhizoctonia solani RNA virus HN008 (RsRV-HN008). ORF2 potentially encodes a 132.41-kDa protein that contains the conserved domain of the RdRp. Phylogenetic analysis indicated that RsRV11 clustered with RsRV-HN008 in a separate clade from other virus families. This implies that RsRV11 and RsRV-HN008 should be included in a new mycovirus taxon close to the family Megabirnaviridae and that RsRV11 is a new mycovirus.
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Acknowledgements
This research was supported the National Natural Scientific Foundation of China (31960525, 31160352, 31360423) and grants-in-aid from the Scientific Research Project of Kunming University (YJL16006) and the General Program of University Union in Yunnan Province (2018FH 001-032).
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Sun, Y., Li, Y., Dong, W. et al. Molecular characterization of a novel mycovirus isolated from Rhizoctonia solani AG-1 IA strain 9-11. Arch Virol 166, 3229–3232 (2021). https://doi.org/10.1007/s00705-021-05219-3
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DOI: https://doi.org/10.1007/s00705-021-05219-3