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Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization

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Abstract

Virus maturation is found in all animal viruses and dsDNA bacteriophages that have been studied. It is a programmed process, cued by cellular environmental factors, that transitions a noninfectious, initial assembly product (provirus) to an infectious particle (virion). Nudaurelia capensis omega virus (NωV) is an ssRNA insect virus with T=4 quasi-symmetry. Over the last 20 years, NωV virus-like particles (VLPs) have been an attractive model for the detailed study of maturation. The novel feature of the system is the progressive transition from procapsid to capsid controlled by pH. Homogeneous populations of maturation intermediates can be readily produced at arbitrary intervals by adjusting the pH between 7.6 and 5.0. These intermediates were investigated using biochemical and biophysical methods to create a stop-frame transition series of this complex process. The studies reviewed here characterized the large-scale subunit reorganization during maturation (the particle changes size from 48 nm to 41 nm) as well as the mechanism of a maturation cleavage, a time-resolved study of cleavage site formation, and specific roles of quasi-equivalent subunits in the release of membrane lytic peptides required for cellular entry.

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Authors and Affiliations

  1. Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA, 92037, USA

    John E. Johnson

  2. Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, 21941-902, Brazil

    Tatiana Domitrovic

  3. Stanford Synchrotron Radiation Lightsource (SSRL), 2575 Sand Hill Rd, MS69, Menlo Park, CA, 94025, USA

    Tsutomu Matsui

  4. Department of Chemistry and Biochemistry, University of California, Los Angeles, 611 Charles Young Dr. East, Los Angeles, CA, 90095-1569, USA

    Roger Castells-Graells

  5. John Innes Centre, The John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK

    George Lomonossoff

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Correspondence to John E. Johnson.

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Johnson, J.E., Domitrovic, T., Matsui, T. et al. Dynamics and stability in the maturation of a eukaryotic virus: a paradigm for chemically programmed large-scale macromolecular reorganization. Arch Virol 166, 1547–1563 (2021). https://doi.org/10.1007/s00705-021-05007-z

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  • DOI: https://doi.org/10.1007/s00705-021-05007-z

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