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Characterization of bacteriophage T7-Ah reveals its lytic activity against a subset of both mesophilic and psychrophilic Aeromonas salmonicida strains

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Abstract

Aeromonas salmonicida strains cause problematic bacterial infections in the aquaculture industry worldwide. The genus Aeromonas includes both mesophilic and psychrophilic species. Bacteriophages that infect Aeromonas spp. strains are usually specific for mesophilic or psychrophilic species; only a few bacteriophages can infect both types of strains. In this study, we characterized the podophage T7-Ah, which was initially found to infect the Aeromonas salmonicida HER1209 strain. The burst size of T7-Ah against its original host is 72 new virions per infected cell, and its burst time is 30 minutes. It has been found that this phage can lyse both mesophilic and psychrophilic A. salmonicida strains, as well as one strain of Escherichia coli. Its genome comprises 40,153 bp of DNA and does not contain any recognizable toxin or antibiotic resistance genes. The adsorption rate of the phage on highly sensitive bacterial strains was variable and could not be related to the presence or absence of a functional A-layer on the surface of the bacterial strains. The lipopolysaccharide migration patterns of both resistant and sensitive bacterial strains were also studied and compared to investigate the nature of the potential receptor of this phage on the bacterial surface. This study sheds light on the surprising diversity of lifestyles of the bacterial strains sensitive to phage T7-Ah and opens the door to the potential use of this phage against A. salmonicida infections in aquaculture.

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Acknowledgements

We would like to thank Sylvain Moineau and Denise Tremblay from the Félix d’Hérelle reference center (U. Laval, Canada). We acknowledge funding from the Ministère de l'agriculture, des pêcheries et de l'alimentation du Québec (INNOVAMER Program), the Natural Sciences and Engineering Research Council of Canada (NSERC) Grant no. RGPIN-2019-04444, and Ressources Aquatiques Québec (RAQ). G.R.L. received an Undergraduate Student Research Award from the NSERC. S.J.C. is a research scholar from the Fonds de Recherche du Québec en Santé (FRQS).

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  1. Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Pavillon Charles-Eugène-Marchand, 1030 avenue de la Médecine, Quebec City, QC, G1V 0A6, Canada

    Gabrielle R. Leduc, Valérie E. Paquet & Steve J. Charette

  2. Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Université Laval, Quebec City, QC, G1V 0A6, Canada

    Gabrielle R. Leduc, Valérie E. Paquet & Steve J. Charette

  3. Centre de recherche de l’Institut universitaire de cardiologie et de pneumologie de Québec (IUCPQ), Quebec City, QC, G1V 4G5, Canada

    Gabrielle R. Leduc, Valérie E. Paquet & Steve J. Charette

  4. Département des sciences animales, Faculté des sciences de l’agriculture et de l’alimentation, Université Laval, 2425, rue de l’Agriculture, Quebec City, QC, G1V 0A6, Canada

    Antony T. Vincent

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  1. Gabrielle R. Leduc
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  2. Valérie E. Paquet
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  3. Antony T. Vincent
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  4. Steve J. Charette
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Correspondence to Steve J. Charette.

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Leduc, G.R., Paquet, V.E., Vincent, A.T. et al. Characterization of bacteriophage T7-Ah reveals its lytic activity against a subset of both mesophilic and psychrophilic Aeromonas salmonicida strains. Arch Virol 166, 521–533 (2021). https://doi.org/10.1007/s00705-020-04923-w

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