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In vivo drug resistance mutation dynamics from the early to chronic stage of infection in antiretroviral-therapy-naïve HIV-infected men who have sex with men

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Abstract

Human immunodeficiency virus type 1 (HIV) primary drug resistance mutations (DRMs) influence the long-term therapeutic effects of antiretroviral treatment (ART). Drug-resistance genotyping based on polymerase gene sequences obtained by next-generation sequencing (NGS) was performed using samples from 10 ART-naïve HIV-infected men who have sex with men (MSM; P1-P10) from the acute/early to chronic stage of infection. Three of the 10 subjects exhibited the presence of major (abundance, ≥ 20%) viral populations carrying DRM at early/acute stage that later, at the chronic stage, dropped drastically (V106M) or remained highly abundant (E138A). Four individuals exhibited additional DRMs (M46I/L; I47A; I54M, L100V) as HIV minority populations (abundance, 2–20%) that emerged during the chronic stage but ephemerally.

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Fig. 1

Data Availability

All HIV-1 pol gene nucleotide sequences from this study are available as a Sequence Read Archive (SRA) with the SRA accession no. PRJNA591115 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA591115/).

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Acknowledgements

Financial support for the conduct of the research was provided by Agencia Nacional de Promoción Científica y Tecnológica (Argentina) from grant PICT-2015-1921 (to JQ). The authors thanks Sergio Mazzini for his assistance during the manuscript preparation.

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Authors and Affiliations

  1. Instituto de Investigaciones Biomédicas en Retrovirus Y Sida (INBIRS), Facultad de Medicina, Universidad de Buenos Aires (UBA), Paraguay 2155—Piso 11 (1121), Buenos Aires, Argentina

    Cintia Cevallos, Javier Urquiza, Diego Ojeda, María M. Avila & Jorge F. Quarleri

  2. Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET) Instituto de Bacteriología Y Virología Molecular (IBaViM) Facultad de Farmacia Y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina

    Andrés C. A. Culasso

  3. Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina

    Andrés C. A. Culasso, Javier Urquiza, Diego Ojeda, María M. Avila, M. Victoria Delpino & Jorge F. Quarleri

  4. Fundación Huésped, Pasaje Angel Peluffo 3932 (C1202ABB), Ciudad Autónoma de Buenos Aires, Argentina

    Omar Sued & María I. Figueroa

  5. Instituto de Inmunología, Genética Y Metabolismo (INIGEM), Universidad de Buenos Aires. CONICET, Buenos Aires, Argentina

    M. Victoria Delpino

Authors
  1. Cintia Cevallos
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  2. Andrés C. A. Culasso
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  3. Javier Urquiza
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  4. Diego Ojeda
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  5. Omar Sued
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  6. María I. Figueroa
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  7. María M. Avila
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  8. M. Victoria Delpino
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  9. Jorge F. Quarleri
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by CC, AC, and JQ. The first draft of the manuscript was written by JQ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Jorge F. Quarleri.

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Cevallos, C., Culasso, A.C.A., Urquiza, J. et al. In vivo drug resistance mutation dynamics from the early to chronic stage of infection in antiretroviral-therapy-naïve HIV-infected men who have sex with men. Arch Virol 165, 2915–2919 (2020). https://doi.org/10.1007/s00705-020-04823-z

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  • DOI: https://doi.org/10.1007/s00705-020-04823-z

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