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Genome sequence of an aichivirus detected in a common pipistrelle bat (Pipistrellus pipistrellus)

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Abstract

The family Picornaviridae includes important human and animal pathogens that are associated with a wide range of diseases and, in some cases, have zoonotic potential. During epidemiological surveillance of bats, we identified, by next-generation sequencing (NGS) techniques, the presence of picornavirus RNA in a common pipistrelle bat (Pipistrellus pipistrellus). By coupling NGS, primer-walking strategies, and sequence-independent protocols to obtain the sequences of the 5′ and 3′ termini, we reconstructed the genome sequence of picornavirus strain ITA/2017/189/18-155. The genome of the bat picornavirus is 8.2 kb in length and encodes a polyprotein of 2462 amino acids. A comparison of polyprotein sequences revealed that this virus is distantly related (65.1% and 70.9% sequence identity at the nucleotide and amino acid level, respectively) to a bat aichivirus identified in 2010. Phylogenetic analysis showed that this picornavirus clustered closely with members of the genus Kobuvirus, which also includes human and animal aichiviruses. The identification of aichiviruses in several animal hosts is providing hints that will lead to an understanding of their origin and evolutionary patterns.

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Funding

This work was supported by the Slovenian Research Agency, Program Group Animal Health, Environment and Food Safety (P4-0092).

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Authors and Affiliations

  1. Department of Veterinary Medicine, University of Bari Aldo Moro, S.p. per Casamassima km3, 70010, Valenzano, Bari, Italy

    Georgia Diakoudi, Gianvito Lanave & Vito Martella

  2. Institute of Food Safety, Feed and Environment, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia

    Urška Jamnikar-Ciglenečki

  3. Istituto Zooprofilattico Sperimentale della Lombardia e dell′Emilia Romagna, Via Bianchi 9 - 25124, Brescia, Italy

    Davide Lelli

  4. Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000, Ljubljana, Slovenia

    Urška Kuhar

Authors
  1. Georgia Diakoudi
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  2. Urška Jamnikar-Ciglenečki
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  3. Gianvito Lanave
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  4. Davide Lelli
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  5. Vito Martella
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  6. Urška Kuhar
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Corresponding author

Correspondence to Vito Martella.

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The Authors declare that they do not have conflict of interest.

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The research did not involve human partecipants and/or experiments/procedures on live animals.

Accession number

The sequence reported here was deposited in the GenBank database under the accession number MN602325.

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Handling Editor: Ana Cristina Bratanich.

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Diakoudi, G., Jamnikar-Ciglenečki, U., Lanave, G. et al. Genome sequence of an aichivirus detected in a common pipistrelle bat (Pipistrellus pipistrellus). Arch Virol 165, 1019–1022 (2020). https://doi.org/10.1007/s00705-020-04548-z

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  • DOI: https://doi.org/10.1007/s00705-020-04548-z

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