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Ultrastructure analysis of white spot syndrome virus (WSSV)

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Abstract

In this study, two aspects of the ultrastructure of white spot syndrome virus (WSSV) were identified: (i) The virus nucleocapsids were disassembled, and transmission electron microscopy (TEM) image analysis confirmed that the nucleocapsids were composed of stacked ring segments rather than the usual helix system, with each ring segment consisting of three rows of subunits linked by filaments. (ii) In addition, the morphological characteristics of virus self-assembly at different stages were observed, and two different enveloping morphologies were found, implying that the virion matures through two distinct envelopments. Thus, we propose a viral membrane assembly process for WSSV virion.

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Acknowledgments

The authors thank Professor Feng Yang from the Key Laboratory of Marine Genetic Resources of the State Oceanic Administration for excellent technical assistance.

Funding

This work was supported by Fujian province “2011 Collaborative Innovation Center” Special Fund (Fujian Education Science [2015] no. 75) and the Discipline Team Construction Fund of Wuyi University (Wu Yuan Zong [2017] no. 66).

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Authors and Affiliations

  1. College of Tea and Food Science, Wuyi University, 358# Baihua Road, Wuyishan, 354300, People’s Republic of China

    Li Li, Yongcong Hong, Da Huo & Pumo Cai

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  1. Li Li
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  2. Yongcong Hong
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Correspondence to Li Li.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Handling Editor: Chan-Shing Lin.

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Li, L., Hong, Y., Huo, D. et al. Ultrastructure analysis of white spot syndrome virus (WSSV). Arch Virol 165, 407–412 (2020). https://doi.org/10.1007/s00705-019-04482-9

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