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Identification and complete-genome phylogenetic analysis of an epizootic hemorrhagic disease virus serotype 7 strain isolated in China

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Abstract

An epizootic hemorrhagic disease virus (EHDV) strain designated YN09-04 was isolated from sentinel cattle in China. The length of its complete genome was 19,344 bp in total, consisting of 10 segments ranging in size from 810 bp (S10) to 3942 bp (S1). Based on phylogenetic analysis of the S2 sequence, YN09-04 clusters with EHDV serotype 7 (EHDV-7) strains form a distinct, well-supported subgroup, indicating that YN09-04 belongs to EHDV-7. However, the origin of the YN09-04 genome is very complex. The S2 and S6 of YN09-04 cluster with those of Japanese EHDV-7 strains, whereas the S1, S3, S4, S5 and S7 of YN09-04 share high nucleotide sequence identity and a close relationship with those of Japanese Ibaraki viruses, and the S8, S9 and S10 nucleotide sequences of YN09-04 are more similar to those of some Australian EHDV strains than to those of other isolates. These results suggest that the genome of YN09-04 likely originated from a reassortment event between EHDV strains that were similar to the current Japanese and Australian strains and that YN09-04 and some EHDVs from Japan and Australia share the same ancestors. This is the first report of the isolation, identification and complete-genome phylogenetic analysis of an EHDV-7 strain from China.

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Acknowledgements

Funding

This study was supported by Special Fund for Agro-Scientific Research in the Public Interest of China (No. 201303035).

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Author notes

  1. Yinglin Qi and Fang Wang contributed equally to this study.

Authors and Affiliations

  1. Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, Heilongjiang, People’s Republic of China

    Yinglin Qi, Fang Wang, Jitao Chang, Yishuang Zhang & Li Yu

  2. Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, 650224, Yunnan, People’s Republic of China

    Jianbo Zhu & Huachun Li

Authors
  1. Yinglin Qi
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  2. Fang Wang
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  3. Jitao Chang
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  4. Yishuang Zhang
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  5. Jianbo Zhu
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  6. Huachun Li
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  7. Li Yu
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Correspondence to Li Yu.

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Qi, Y., Wang, F., Chang, J. et al. Identification and complete-genome phylogenetic analysis of an epizootic hemorrhagic disease virus serotype 7 strain isolated in China. Arch Virol 164, 3121–3126 (2019). https://doi.org/10.1007/s00705-019-04412-9

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  • DOI: https://doi.org/10.1007/s00705-019-04412-9

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