Skip to main content
Log in

Complete genome sequence of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

The complete genome of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli, was sequenced, revealing a linear double-stranded DNA genome of 48,939 bp with 46% GC content and protruding 150-bp 5ʹ cohesive termini. The genome contained 85 open reading frames, 28 of which were annotated with known functions. No tRNA-encoding genes were detected. Phylogenetic analysis suggested that phage PHB17 is a novel phage of family Siphoviridae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Castro VS, Figueiredo EES, Stanford K, McAllister T, Conte-Junior CA (2019) Shiga-Toxin Producing Escherichia Coli in Brazil: a systematic review. Microorganisms 7(5):pii: E137

    Article  Google Scholar 

  2. Wong CS, Jelacic S, Habeeb RL, Watkins SL, Tarr PI (2000) The risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections. N Engl J Med 342(26):1930–1936

    Article  CAS  Google Scholar 

  3. Chen Y, Yang L, Sun E, Song J, Wu B (2018) Characterisation of a newly detected bacteriophage infecting Bordetella bronchiseptica in swine. Arch Virol 164:33–40

    Article  Google Scholar 

  4. Chen Y, Guo G, Sun E, Song J, Yang L, Zhu L, Liang W, Hua L, Peng Z, Tang X, Chen H, Wu B (2019) Isolation of a T7-Like Lytic Pasteurella bacteriophage vB_PmuP_PHB01 and Its potential use in therapy against Pasteurella multocida Infections. Viruses. https://doi.org/10.3390/v11010086

    Article  PubMed  PubMed Central  Google Scholar 

  5. Li S, Fan H, An X, Fan H, Jiang H, Chen Y, Tong Y (2014) Scrutinizing virus genome termini by high-through put sequencing. PLoS One 9:e85806

    Article  Google Scholar 

  6. Zhang X, Wang Y, Tong Y (2018) Analyzing genome termini of bacteriophage through high-throughput sequencing. Methods Mol Biol 1681:139–163

    Article  CAS  Google Scholar 

  7. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

  8. Piligrimova EG, Kazantseva OA, Nikulin NA, Shadrin AM (2019) Bacillus phage vB_BtS_B83 previously designated as a plasmid may represent a new Siphoviridae genus. Viruses. https://doi.org/10.3390/v11070624

    Article  PubMed  PubMed Central  Google Scholar 

  9. Kyrkou I, Byth Carstens A, Ellegaard-Jensen L, Kot W, Zervas A, Djurhuus AM, Neve H, Hansen M, Hestbjerg Hansen L (2019) Expanding the diversity of myoviridae phages infecting Lactobacillus plantarum—a novel lineage of Lactobacillus phages comprising five new members. Viruses. https://doi.org/10.3390/v11070611

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This work was supported by grants from the National Program on Key Research Project of China (2018YFD0500204), the Technology Base and Talents Special Program of Guangxi Province (2018AD09007), and the Innovation and Entrepreneurship Training Program for College Students in Hubei Province (no. S201910504011).

Author information

Authors and Affiliations

Authors

Contributions

Y. Chen drafted the manuscript. Y. Chen, S. Wang, X. Zhang, and X. Li performed the data analysis. P. Qian and H. Chen were responsible for experimental design. All authors reviewed and agreed to the publication of this manuscript.

Corresponding author

Correspondence to Ping Qian.

Ethics declarations

Conflict of interest

There are no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: T. K. Frey.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Y., Wang, S., Wang, Y. et al. Complete genome sequence of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli. Arch Virol 164, 3111–3113 (2019). https://doi.org/10.1007/s00705-019-04402-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-019-04402-x

Navigation