Abstract
A lytic bacteriophage, designated Vibrio phage vB_VpP_BA6, was isolated from sewage collected in Guangzhou, China. The double-stranded DNA genome of phage BA6 is composed of 50,520 bp with a G+C content of 41.77%. It possesses 64 open reading frames relating to phage structure, packaging, host lysis, DNA metabolism, and additional functions. Three tRNAs genes (encoding Pro, Ile and Trp) were detected. Comparison of its genomic features and phylogenetic analysis revealed that phage BA6 is a novel member of the family Podoviridae. This phage may represent a potential therapeutic agent against multidrug-resistant Vibrio parahaemolyticus.
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Acknowledgements
The authors are grateful to the National Key R&D Program of China (2017YFC1601201), the GDAS’ Project of Science and Technology Development (No. 2019GDASYL-0103011), the Key Project of Natural Science Foundation of China (31730070) and GDAS’ Special Project of Science and Technology Development (2017GDASCX-0201) for financial support.
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Supplementary Fig. S1
Transmission electron microscopy image of vB_VpP_BA6
Supplementary Fig. S2
Terminal sequence determination in the vB_VpP_BA6 genome. The panels show the run-off sequencing spectrograms of the phage vB_VpP_BA6 genome using a forward primer (Forward: 5’-CTGGTGGAAGAACT GTAGAA-3′) and a reverse primer (Reverse: 5’-ATACCCGTTGCTTATCATC-3’). (A) The 3’-terminal sequence. (B) The 5’-terminal sequence
Supplementary Table S1
Lytic spectra of vB_VpP_BA6 based on 30 bacterial strains of Vibrio parahaemolyticus. Clear lysis zone (+), no lysis zone (−)
Supplementary Table S2
Open reading frames in the phage vB_VpP_BA6 genome
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Yang, M., Liang, Y., Su, R. et al. Genome characterization of the novel lytic Vibrio parahaemolyticus phage vB_VpP_BA6. Arch Virol 164, 2627–2630 (2019). https://doi.org/10.1007/s00705-019-04351-5
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DOI: https://doi.org/10.1007/s00705-019-04351-5