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Phylogenetic analysis of novel posaviruses detected in feces of Japanese pigs with posaviruses and posa-like viruses of vertebrates and invertebrates

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Abstract

Posaviruses and posa-like viruses are unclassified viruses with sequence similarity to viruses of the order Picornavirales. They have been reported in various vertebrates and invertebrates. We identified 11 posavirus-like sequences in porcine feces and performed phylogenic analysis. Previously reported Japanese posaviruses and those identified in this study clustered with posavirus 1, 4, and 7 and husavirus 1, while five viruses branched into three independent lineages, tentatively named posavirus 10, 11, and 12. Interestingly, posaviruses, except for posavirus 8 and 9, husaviruses, and rasaviruses, formed a cluster consisting of viruses only from pigs, humans, and rats, while posavirus 8 and 9, fisavirus, and basaviruses clustered with posa-like viruses from invertebrates.

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Acknowledgements

This work was supported by JSPS KAKENHI (Grant numbers: 15K07718 and 18K05977).

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Author notes

  1. Hiroshi Aoki and Fujiko Sunaga contributed equally to this work.

Authors and Affiliations

  1. Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo, 180-8602, Japan

    Hiroshi Aoki

  2. School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan

    Fujiko Sunaga, Hideharu Ochiai & Makoto Nagai

  3. Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, 683-0017, Japan

    Tsuneyuki Masuda

  4. Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa, 920-3101, Japan

    Mika Ito

  5. Kenpoku Livestock Hygiene Service Center, Mito, Ibaraki, 310-0002, Japan

    Masataka Akagami & Yoshinao Ouchi

  6. Research and Education Center for Prevention of Global Infectious Disease of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, 183-8509, Japan

    Yuki Naoi, Kaori Sano, Yukie Katayama, Tsutomu Omatsu, Mami Oba, Shoichi Sakaguchi, Tetsuya Mizutani & Makoto Nagai

  7. Department of Microbiology and Infection Control, Osaka Medical College, Osaka, 569-8686, Japan

    Shoichi Sakaguchi

  8. Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Tetsuya Furuya & Junsuke Shirai

  9. Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo, 208-0011, Japan

    Tomoichiro Oka

Authors
  1. Hiroshi Aoki
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  2. Fujiko Sunaga
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  3. Hideharu Ochiai
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  15. Junsuke Shirai
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  16. Tetsuya Mizutani
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  17. Tomoichiro Oka
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  18. Makoto Nagai
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Corresponding author

Correspondence to Makoto Nagai.

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This study did not involve any human participants or animals.

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Handling Editor: Helene Sanfacon.

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Supplementary Fig.

Genome structure of Ishi-Im7/JPN/2016 with the putative conserved domains identified using the CCD search. (PPTX 37 kb)

Supplementary material 2 (XLSX 18 kb)

Supplementary material 3 (TXT 99 kb)

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Aoki, H., Sunaga, F., Ochiai, H. et al. Phylogenetic analysis of novel posaviruses detected in feces of Japanese pigs with posaviruses and posa-like viruses of vertebrates and invertebrates. Arch Virol 164, 2147–2151 (2019). https://doi.org/10.1007/s00705-019-04289-8

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  • DOI: https://doi.org/10.1007/s00705-019-04289-8

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